The Keap1-Nrf2 signaling pathway regulates antioxidant defenses of Ctenopharyngodon idella induced by bacterial infection

Li, Yanan; Chen, Zongfu; Xiao, Yayuan; Gao, Feng; Lu, Zhijie; Huang, Zhenpeng; Wei, Xuefeng; Su, Fengping; Shi, Fei; Lin, Li; Qin, Zhendong

doi:10.1016/j.fsi.2023.108686

Cited by 2 publications

(4 citation statements)

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“…In this study, the antioxidant activity of five SVP polysaccharides was determined in vitro by antioxidant experiments, including the DPPH radial scavenging ability, hydroxyl radial scavenging ability, superoxide anion radical scavenging ability, and reducing ability. These findings further demonstrate that the in vivo biological activity of S. vaninii needs to be further explored in terms of the specific antioxidant indicators SOD and CAT [ 39 ] and the related protein pathway Nrf-2/Keap-1 [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

“…Considering the energy consumption and extraction cost, an optimal extraction time of 3 h is sufficient to obtain maximal polysaccharide extraction. During UAE, the extraction times were set to 30,40,50,60, and 70 min. The other extraction parameters were as follows: a liquid-solid ratio of 40 mL/g, an extraction temperature of 60 • C, and an ultrasonic power of 70 W. As shown in Figure 1b, the extraction time increased from 30 to 60 min, and the extraction rate gradually decreased from 60 min.…”

Section: Effect Of the Extraction Time On The Extraction Rate Of Svpmentioning

confidence: 99%

“…An appropriate amount of the SVP sample was spread on conductive silica gel, sprayed with gold, placed on a SU8020 electric scanner, and recorded. The working voltage was set to 5.0 kV, and the solid shape of the sample was observed at different magnification appearances [69] (1,5,10,20,40,50,60,70,80, 100, 500, 1000 µg/mL) were cultured for 24 h. We added 100 µL CCK-8 per well and incubated for 1 h. The cytotoxic effects of all samples were evaluated. Each dose had three repetitions.…”

Section: Detection Of Purified Polysaccharide By Scanning Electron Mi...mentioning

confidence: 99%

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Extraction, Purification, and Structural Characterization of Polysaccharides from Sanghuangporus vaninii with Anti-Inflammatory Activity

et al. 2023

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In order to obtain homogeneous Sanghuangporus vaninii polysaccharides with antioxidant and anti-inflammatory activities, a response surface method (RSM) was used to compare the polysaccharide extraction rate of hot water extraction and ultrasonic-assisted extraction from Sanghuangporus vaninii. The optimal conditions for ultrasonic-assisted extraction were determined as follows: an extraction temperature of 60 °C, an extraction time of 60 min, a solid–liquid ratio of 40 g/mL, and an ultrasonic power of 70 W. An SVP (Sanghuangporus vaninii polysaccharides) extraction rate of 1.41% was achieved. Five homogeneous monosaccharides were obtained by gradient ethanol precipitation with diethylaminoethyl–cellulose (DEAE) and SephadexG-100 separation and purification. The five polysaccharides were characterized by high performance liquid chromatography, the ultraviolet spectrum, the Fourier transform infrared spectrum, TG (thermogravimetric analysis), the Zeta potential, and SEM (scanning electron microscopy). The five polysaccharides had certain levels of antioxidant activity in vitro. In addition, we the investigated the anti-inflammatory effects of polysaccharides derived from Sanghuangporus vaninii on lipopolysaccharide (LPS)-induced RAW 264.7 cells and Kupffer cells. Further, we found that SVP-60 significantly inhibited the levels of pro-inflammatory cytokines, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in lipopolysaccharide (LPS)-induced RAW 264.7 cells and promoted the level of the anti-inflammatory cytokine IL-10 in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Our study provides theoretical support for the potential application of Sanghuangporus vaninii in the field of antioxidant and anti-inflammatory activities in vitro.

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Section: Discussionmentioning

confidence: 99%

Section: Effect Of the Extraction Time On The Extraction Rate Of Svpmentioning

confidence: 99%

Section: Detection Of Purified Polysaccharide By Scanning Electron Mi...mentioning

confidence: 99%

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Extraction, Purification, and Structural Characterization of Polysaccharides from Sanghuangporus vaninii with Anti-Inflammatory Activity

et al. 2023

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“…While GST genes exhibited different expression tendencies with the increasing of dietary protein level, the Nrf2-keap 1-ARE signaling pathway is the most important endogenous antioxidant signaling pathway in current organisms [32]. Nrf2 is the most important transcription factor in the cell oxidative stress pathway and can increase the expression level of a variety of antioxidant proteins and phase detoxification enzymes by interacting with antioxidant response elements, thereby removing free radicals produced by oxidative stress in the body so as to maintain the redox state of cells [33,34]. Both mRNA and proteins of triploid crucian carp Nrf2 declined with the increase in dietary protein inclusion level, which suggests that the increasing dietary protein inclusion level would reduce the antioxidant level of triploid crucian carp.…”

Section: Discussionmentioning

confidence: 99%

Intervention of Dietary Protein Levels on Muscle Quality, Antioxidation, and Autophagy in the Muscles of Triploid Crucian Carp (Carassius carassius Triploid)

Cai

Yang

et al. 2023

IJMS

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The aim of this study is to investigate the effect of dietary protein levels on flesh quality, oxidative stress, and autophagy status in the muscles of triploid crucian carp (Carassius carassius triploid), and the related molecular mechanisms. Six experimental diets with different protein levels (26%, 29%, 32%, 35%, 38%, 41%) were formulated. A total of 540 fish with an initial weight of 11.79 ± 0.09 g were randomly assigned to 18 cages and six treatments with three replicates of 30 fish each for 8 weeks feeding. It could be found that the whole-body ash content significantly increased in high protein level groups (p < 0.05). The 29% dietary protein level group exhibited the highest muscle moisture, although there was an inconspicuous decrease in the chewiness of the muscles when compared with the other groups. The dietary protein level influenced the content of free amino acids and nucleotides, especially the content of flavor amino acids, which exhibited an increasing tendency along with the increasing protein level, such as alanine and glutamic acid, while the flavor nucleotides showed different fluctuation trends. Moreover, the genes related to muscle development were shown to be influenced by the dietary protein level, especially the expression of MRF4, which was up-regulated with the increasing dietary protein levels. The 29% dietary protein level promoted the majority of analyzed muscle genes expression to the highest level when compared to other dietary levels, except the Myostain, whose expression reached its highest at 38% dietary protein levels. Furthermore, the effect of dietary protein levels on antioxidant signaling pathway genes were also examined. High protein levels would boost the expression of GSTα; GPX1 and GPX4α mRNA expression showed the highest level at the 32% dietary protein group. The increasing dietary protein level decreased both mRNA and protein expressions of Nrf2 by up-regulating Keap1. Autophagy-related gene expression levels reached the peak at 32% dietary protein level, as evidenced by a similar change in protein expression of FoxO1. In summary, muscle nutritional composition, antioxidative pathways, and autophagy levels were affected by the dietary protein levels. A total of 29–32% dietary protein level would be the appropriate level range to improve muscle quality and promote the antioxidant and autophagy capacity of triploid crucian carp muscles.

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The Keap1-Nrf2 signaling pathway regulates antioxidant defenses of Ctenopharyngodon idella induced by bacterial infection

Cited by 2 publications

References 46 publications

Extraction, Purification, and Structural Characterization of Polysaccharides from Sanghuangporus vaninii with Anti-Inflammatory Activity

Extraction, Purification, and Structural Characterization of Polysaccharides from Sanghuangporus vaninii with Anti-Inflammatory Activity

Intervention of Dietary Protein Levels on Muscle Quality, Antioxidation, and Autophagy in the Muscles of Triploid Crucian Carp (Carassius carassius Triploid)

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