The K428 residue fromThermus thermophilusSG0.5JP17-16 laccase plays the substantial role in substrate binding and oxidation

Electrochemistry of copper efflux oxidase-like multicopper oxidases involved in copper homeostasis

“…The fact that the deletion of the Met-rich domain in Tt LAC did not suppress the Cu-induced electrocatalytic process, and that Met residues in the coordination sphere of CuT1 were also involved in the Cu 2+ -activation process further support this conclusion [22,46]. An alternative role for the Met-rich domain of CueOs, which is closely related, is linked to the flexibility of the Met-rich domain with an even enhanced flexibility upon Cu binding, as demonstrated in CueO enzymes [26,[62][63][64][65]. However, this flexibility hasn't been yet demonstrated to influence the electrochemical behavior.…”

Phenoloxidases: catechol oxidase – the temporary employer and laccase – the rising star of vascular plants

“…Laccases contain four copper ions: type 1 (T1), type 2 (T2), and double type 3 (T3α, T3β). The amino acids surrounding the substrate-binding pocket near the T1 Cu center of domain 3 influence the substrate selectivity and catalytic properties [ 31 – 33 ]. We further extracted and compared these amino acid residues to explore the origin and evolution of LACs; the amino acids of four potential LACs of Zygnemaphyceae were more like those of the identified LACs in bryophytes ( Fig.…”

Current status and emerging frontiers in enzyme engineering: An industrial perspective

“… Site directed mutagenesis Applied in production industries [ 125 ] Chitinase Paenibacillus pasadenesis CS0611 Site directed mutagenesis Chito-oligosaccharides production [ 126 ] Type II pullulunase Anoxybacillus sp. WB 42 Site-directed mutagenesis utilizing overlap extension PCR Used in chemical and food industries for debranching of starch [ 127 ] Laccase Thermus thermophilus SG0.5JP17-16 Site directed mutagenesis The manufacturing of paper and its usage in the food companies [ 128 ] α-amylase Klebsiella pneumoniae (CCICC no. 10018) Site-saturation mutagenesis Application in food processing and biosynthesis [ 129 ] Pullulanase Bacillus acidopullulyticus Site directed mutagenesis The enzymatic hydrolysis of starch for the purpose of producing high-glucose syrup [ 130 ] β-1,4-glucosidase chimeric enzyme and Endo-glucanase Clostridium thermocellum Site directed mutagenesis Biofuel and ioethanol production [ 131 ] …”