The juvenile three-spined stickleback (Gasterosteus aculeatus L.) as a model organism for endocrine disruption II—kidney hypertrophy, vitellogenin and spiggin induction

Hahlbeck, Edda; Katsiadaki, Ioanna; Mayer, Ian; Adolfsson-Erici, Margaretha; James, Jonathan; Bengtsson, Bengt‐Erik

doi:10.1016/j.aquatox.2004.10.004

Cited by 68 publications

(44 citation statements)

References 52 publications

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“…Stickleback is a model fish used to addres questions related to adaptation, speciation, and evolution (Bell 2001;Cresko et al 2007). It is a small fish (adult total body length\10 cm), survives and breeds in both seawater and freshwater; displays a variety of pronounced reproductive behaviors; has a simple and short life cycle of 1 year in nature, but can be brought to sexual maturity in the laboratory within 7-8 months after hatching (Hahlbeck et al 2004;Katsiadaki et al 2002).…”

Section: Relationships Of Zebrafish and Other Fish Modelsmentioning

confidence: 99%

Zebrafish as a model organism for nutrition and growth: towards comparative studies of nutritional genomics applied to aquacultured fishes

Ulloa

Iturra

Neira

et al. 2011

Rev Fish Biol Fisheries

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Zebrafish (Danio rerio) is a common research model in fish studies of toxicology, developmental biology, neurobiology and molecular genetics; it has been proposed as a possible model organism for nutrition and growth studies in fish. The advantages of working with zebrafish in these areas are their small size, short generation time (12-14 weeks) and their capacity to produce numerous eggs (100-200 eggs/clutch). Since a wide variety of molecular tools and information are available for genomic analysis, zebrafish has also been proposed as a model for nutritional genomic studies in fish. The detailed study of every species employed as a model organism is important because these species are used to generalize how several biological processes occur in related organisms, and contribute considerably toward improving our understanding of the mechanisms involved in nutrition and growth. The objective of this review is to show the relevant aspects of the nutrition and growth in zebrafish that support its utility as a model organism for nutritional genomics studies. We made a particular emphasis that gene expression and genetic variants in response to zebrafish nutrition will shed light on similar processes in aquacultured fish.

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Section: Relationships Of Zebrafish and Other Fish Modelsmentioning

confidence: 99%

Zebrafish as a model organism for nutrition and growth: towards comparative studies of nutritional genomics applied to aquacultured fishes

Ulloa

Iturra

Neira

et al. 2011

Rev Fish Biol Fisheries

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“…The traditional toxicological approaches focus on the measure of specific biological or biochemical responses (biomarkers), such as antioxidant enzymatic activities to test for oxidative stress and the expression of the protein vitellogenin to assess the endocrine disruption of contaminants (Hahlbeck et al, 2004;Hansen et al, 1998). Due to the recent developments in system biology (genomics, proteomics, transcriptomics and metabolomics), the approaches for toxicological biomarker discovery have been greatly expanded for the global biomarker discoveries (Daviss, 2005).…”

Section: Introductionmentioning

confidence: 99%

Benzo(a)pyrene-induced metabolic responses in Manila clam Ruditapes philippinarum by proton nuclear magnetic resonance (1H NMR) based metabolomics

Zhang

Liu

You

et al. 2011

Environmental Toxicology and Pharmacology

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“…A homologous stickleback VTG ELISA was used; this has been validated previously and has a high specificity and sensitivity (Katsiadaki et al,. 2002b;Hahlbeck et al,. 2004).…”

mentioning

confidence: 99%

Field surveys reveal the presence of anti-androgens in an effluent-receiving river using stickleback-specific biomarkers

et al. 2012

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Article (refereed) -postprintKatsiadaki, Ioanna; Sanders, Matthew B.; Henrys, Peter A.; Scott, Alexander P.; Matthiessen, Peter; Pottinger, Tom G. 2012 Field surveys reveal the presence of anti-androgens in an effluent-receiving river using sticklebackspecific biomarkers. Aquatic Toxicology, 122-123. 75-85. 10.1016Toxicology, 122-123. 75-85. 10. /j.aquatox.2012 Contact CEH NORA team at noraceh@ceh.ac.ukThe NERC and CEH trademarks and logos ('the Trademarks') are registered trademarks of NERC in the UK and other countries, and may not be used without the prior written consent of the Trademark owner.Field surveys reveal the presence of anti-androgens in an effluentreceiving river using stickleback-specific biomarkers AbstractThis study was designed to assess whether the removal of endocrine disrupting chemicals (EDCs) and other substances from a Waste Water Treatment Works (WWTW) effluent (receiving water: R. Ray, Swindon, UK) by granular activated carbon (GAC) affected biomarkers of exposure to EDCs [vitellogenin (VTG) and spiggin] in male and female three-spined sticklebacks in the receiving water. A nearby river (R. Ock), with a negligible effluent loading, was used as a control. On each river fish were sampled from four sites on five occasions both before and after remediation of the WWTW effluent. The results show for the first time in a UK field study a clear seasonality of blood VTG concentrations in wild male fish, following closely the VTG profile in female fish from both rivers. VTG levels in male fish from the R. Ray were significantly reduced after the GAC installation. However, VTG levels in males from the control sites also varied significantly across the same period, reducing the significance of this finding. A laboratory exposure to oestradiol (using site-specific lower and upper levels of oestrogenic activity) failed to elevate VTG concentrations in male sticklebacks suggesting that concentrations in the effluent, even prior to remediation, may not have exceeded a critical sensitivity threshold.Most importantly, a significant increase in female kidney spiggin content (a highly specific biomarker of xeno-androgen exposure) occurred in fish in the R. Ray after the GAC installation to levels comparable with those in fish from the control river. The significance of this finding is strengthened by the fact that during the pre-remediation period in the R. Ray, female spiggin levels increased with increasing distance from the WWTW. Our results provide the first in vivo evidence of the presence of antiandrogens in a U.K. WWTW effluent. To our knowledge this is the first U.K.-based comprehensive field study on the effects of a WWTW upgrade on biomarkers of EDC exposure using a sentinel fish species and our findings confirm the value of the stickleback as a model species for studying EDCs both in the laboratory and in the wild.Key words: endocrine disruption, wild fish survey, vitellogenin, spiggin. IntroductionEndocrine disrupting chemicals (EDCs) comprise a large group of natural and synthetic substan...

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The juvenile three-spined stickleback (Gasterosteus aculeatus L.) as a model organism for endocrine disruption II—kidney hypertrophy, vitellogenin and spiggin induction

Cited by 68 publications

References 52 publications

Zebrafish as a model organism for nutrition and growth: towards comparative studies of nutritional genomics applied to aquacultured fishes

Zebrafish as a model organism for nutrition and growth: towards comparative studies of nutritional genomics applied to aquacultured fishes

Benzo(a)pyrene-induced metabolic responses in Manila clam Ruditapes philippinarum by proton nuclear magnetic resonance (1H NMR) based metabolomics

Field surveys reveal the presence of anti-androgens in an effluent-receiving river using stickleback-specific biomarkers

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