The JMJD6/HURP axis promotes cell migration via NF‐κB‐dependent centrosome repositioning and Cdc42‐mediated Golgi repositioning

Abstract

Golgi apparatus (GA) and centrosome reposition toward cell leading end during directional cell migration in a coupling way, thereby determining cell polarity by transporting essential factors to the proximal plasma membrane. The study provides mechanistic insights into how GA repositioning (GR) is regulated, and how GR and centrosome repositioning (CR) are coupled. Our previous published works reveals that PRMT5 methylates HURP at R122 and the HURP m122 inhibits GR and cell migration by stabilizing GA‐associated acetyl‐tubulin and then rigidifying GA. The current study further shows that the demethylase JMJD6‐guided demethylation of HURP at R122 promotes GR and cell migration. The HURP methylation mimicking mutant 122 F blocks JMJD6‐induced GR and cell migration, suggesting JMJD6 relays GR stimulating signal to HURP. Mechanistic studies reveal that the HURP methylation deficiency mutant 122 K promotes GR through NF‐κB‐induced CR and subsequently CR‐dependent Cdc42 upregulation, where Cdc42 couples CR to GR. Taken together, HURP methylation statuses provide a unique opportunity to understand how GR is regulated, and the GA intrinsic mechanism controlling Golgi rigidity and the GA extrinsic mechanism involving NF‐κB‐CR‐Cdc42 cascade collectively dictate GR.

The crescent-like Golgi ribbon is shaped by the Ajuba/PRMT5/Aurora-A complex-modified HURP

“…To analyze the structure of GA or HURP p725, 200 cells for each independent experiment were examined, and 3 independent experiments were performed. The GA structure was classified as the following criteria according to published papers [ 21 , 49 ]: Ribbon, GA with continuous crescent shape; Compact, GA with ball-like appearance; Fragmentation, GA with discontinuous fragments scattering around the nucleus or cytoplasm. As to the measurement of the length of HURP p725 or GA, 20 cells were examined with the image analysis software MetaVue version 7.8.0.0 (Molecular Devices) for each independent experiment and 3 independent experiments were performed.…”