The Jatropha leaf curl Gujarat virus on infection in Jatropha regulates the sugar and tricarboxylic acid cycle metabolic pathways

More, Prashant; Agarwal, Parinita; Agarwal, Pradeep K.

doi:10.1007/s13205-022-03306-z

Cited by 3 publications

(1 citation statement)

References 34 publications

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“…There exists a wealth of research conducted on the altered metabolic state of entire symptomatic plant leaves during virus infection using metabolomics (Choi et al, 2006; Lionel et al, 2012; Mandal et al, 2012; López-Gresa et al, 2012; Palama et al, 2012; Plischke et al, 2012; Manchester and Anand, 2017; Villa-Ruano et al, 2018; Rossouw et al, 2019; Velásquez-Valle et al, 2020; J. Mishra et al, 2020; Maravi et al, 2022; More et al, 2022; Zhang et al, 2022). However, there remains a notable dearth of studies investigating the precise changes in the apoplast and cytoplasm region-specific metabolite composition during viral infection.…”

Section: Introductionmentioning

confidence: 99%

Identification of Begomovirus Genomic Components and Stress-specific Metabolic Markers in Mungbean Leaf Apoplast

Dhobale,

Sahoo

2023

Preprint

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Plant viruses exert control over the host metabolism to enhance their survival, but the specific sites where altered metabolites take effect remain enigmatic. This study focuses on the apoplastic region of symptomatic mungbean leaves infected with mungbean yellow mosaic India virus (MYMIV) to gain new insights into metabolite changes during infection. Leveraging NMR-based metabolome fingerprinting, we identified several stress-associated metabolites. Notably, proline and 2-Hydroxybutyrate were found to be up-regulated in the apoplast region, while down-regulated in the cytosol. Additionally, aspartate was found to be upregulated in the cytosolic region but absent in the apoplast. Importantly, our molecular analysis of the apoplast suggests the presence of MYMIV genomic components. Moreover, we characterized two distinct populations of extracellular vesicles (EVs) using ultracentrifugation, transmission electron microscopy, dynamic light scattering, and fluorometric assays. The data revealed alterations in the size and count of EVs, suggesting their potential role in facilitating the transport of viral components for long-distance cell-to-cell movement during infection. These findings provide valuable insights into the apoplast's role and the significance of EVs in MYMIV infection, contributing to a better understanding of virus-host interactions and potentially informing new virus defense strategies.

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