The jasmonate biosynthesis Gene OsOPR7 can mitigate salinity induced mitochondrial oxidative stress

Asfaw, Kinfemichael Geressu; Liu, Qiong; Eghbalian, Rose; Purper, Sabine; Akaberi, Sahar; Dhakarey, Rohit; Münch, Stephan W.; Wehl, Ilona; Bräse, Stefan; Eiche, Elisabeth; Hause, Bettina; Bogeski, Ivan; Schepers, Ute; Riemann, Michael; Nick, Peter

doi:10.1016/j.plantsci.2021.111156

Cited by 10 publications

(6 citation statements)

References 55 publications

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“…However, in our work, we did not observe a significant decrease in the level of 12-OPDA in either hexaploid or tetraploid transgenic wheat lines, and, therefore, we cannot assume that the observed decrease in the tolerance to the pathogen is due to a decrease in this metabolite. Our data are consistent with those obtained on rice; in contrast to expectation, the overexpression of OsOPR7 in rice does not reduce the steady-state levels of 12-OPDA, but rather leads to an increase [ 35 ]. The question about the role of 12-OPDA in the regulation of tolerance to pathogens requires further study using plants in which the level of 12-OPDA is markedly altered.…”

Section: Discussionsupporting

confidence: 92%

“…The basal levels of 12-OPDA in both Sar-60 and Runo were below 20 pmol/g f.w., while the levels of 12-OPDA in Arabidopsis leaves varied between 0.5 and 10 nmol/g f.w., according to the different sources [ 38 , 40 , 41 ], which was tens or hundreds-fold higher than the values obtained in wheat. Detected levels of 12-OPDA were also much lower than those previously obtained for another monocotyledonous crop, rice, using the same analytical equipment and metabolite extraction and analysis methods [ 35 , 42 ]. Wounding led to an increase in the levels of all quantified jasmonates in both wheat species studied, but the magnitude of changes in the hormone content was also much smaller than that described for Arabidopsis, in which the wounding led to an increase in the levels of JA by a hundredfold or more.…”

Section: Discussionmentioning

confidence: 67%

“…The OPR gene is one of the promising targets for genetic manipulation. Overexpression of OsOPR7 , coding for the peroxisome-localized enzyme, in rice was shown to mitigate salinity stress [ 35 ]. In a recent study [ 36 ], the authors show that the gene dosage or expression level of monocot-specific OPR subfamily III members affects root architecture and their ability to access water under limited water conditions.…”

Section: Discussionmentioning

confidence: 99%

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12-Oxophytodienoate Reductase Overexpression Compromises Tolerance to Botrytis cinerea in Hexaploid and Tetraploid Wheat

Degtyaryov,

Pigolev,

Miroshnichenko

et al. 2023

Plants

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12-Oxophytodienoate reductase is the enzyme involved in the biosynthesis of phytohormone jasmonates, which are considered to be the major regulators of plant tolerance to biotic challenges, especially necrotrophic pathogens. However, we observe compromised tolerance to the necrotrophic fungal pathogen Botrytis cinerea in transgenic hexaploid bread wheat and tetraploid emmer wheat plants overexpressing 12-OXOPHYTODIENOATE REDUCTASE-3 gene from Arabidopsis thaliana, while in Arabidopsis plants themselves, endogenously produced and exogenously applied jasmonates exert a strong protective effect against B. cinerea. Exogenous application of methyl jasmonate on hexaploid and tetraploid wheat leaves suppresses tolerance to B. cinerea and induces the formation of chlorotic damages. Exogenous treatment with methyl jasmonate in concentrations of 100 µM and higher causes leaf yellowing even in the absence of the pathogen, in agreement with findings on the role of jasmonates in the regulation of leaf senescence. Thereby, the present study demonstrates the negative role of the jasmonate system in hexaploid and tetraploid wheat tolerance to B. cinerea and reveals previously unknown jasmonate-mediated responses.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

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12-Oxophytodienoate Reductase Overexpression Compromises Tolerance to Botrytis cinerea in Hexaploid and Tetraploid Wheat

Degtyaryov,

Pigolev,

Miroshnichenko

et al. 2023

Plants

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“…Deficiency in A biosynthesis decreased the salt tolerance of maize shoots ( Ahmad et al., 2019 ) and tomato ( Abouelsaad and Renault, 2018 ). Furthermore, overexpression of JA biosynthesis genes of rice in tobacco effectually promoted its salt tolerance ( Asfaw et al., 2022 ). These findings confirmed the beneficial regulatory functions of JA in enhancing plant resilience to salt stress.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis reveals that methyl jasmonate confers salt tolerance in alfalfa by regulating antioxidant activity and ion homeostasis

Yin,

Yang,

et al. 2023

Front. Plant Sci.

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IntroductionAlfalfa, a globally cultivated forage crop, faces significant challenges due to its vulnerability to salt stress. Jasmonates (JAs) play a pivotal role in modulating both plant growth and response to stressors.MethodsIn this study, alfalfa plants were subjected to 150 mM NaCl with or without methyl jasmonate (MeJA). The physiological parameters were detected and a transcriptomic analysis was performed to elucidate the mechanisms underlying MeJA-mediated salt tolerance in alfalfa.ResultsResults showed that exogenous MeJA regulated alfalfa seed germination and primary root growth in a dose-dependent manner, with 5µM MeJA exerting the most efficient in enhancing salt tolerance. MeJA at this concentration elavated the salt tolerance of young alfalfa seedlings by refining plant growth, enhancing antioxidant capacity and ameliorating Na+ overaccumulation. Subsequent transcriptomic analysis identified genes differentially regulated by MeJA+NaCl treatment and NaCl alone. PageMan analysis revealed several significantly enriched categories altered by MeJA+NaCl treatment, compared with NaCl treatment alone, including genes involved in secondary metabolism, glutathione-based redox regulation, cell cycle, transcription factors (TFs), and other signal transductions (such as calcium and ROS). Further weighted gene co-expression network analysis (WGCNA) uncovered that turquoise and yellow gene modules were tightly linked to antioxidant enzymes activity and ion content, respectively. Pyruvate decar-boxylase (PDC) and RNA demethylase (ALKBH10B) were identified as the most central hub genes in these two modules. Also, some TFs-hub genes were identified by WGCNA in these two modules highly positive-related to antioxidant enzymes activity and ion content.DiscussionMeJA triggered a large-scale transcriptomic remodeling, which might be mediated by transcriptional regulation through TFs or post-transcriptional regulation through demethylation. Our findings contributed new perspectives for understanding the underneath mechanisms by which JA-mediated salt tolerance in alfalfa.

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“…For instance, in wheat, overexpression of TaOPR1 has been found to enhance plant tolerance to salinity in an ABA-dependent manner [20]. Similarly, overexpression of OsOPR7 in tobacco has been shown to alleviate salinity-induced mitochondrial oxidative damage [21]. Furthermore, transgenic wheat plants with increased AtOPR3 expression have delayed germination, growth, blooming, and senescence.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification, gene cloning, subcellular location and expression analysis of the OPR gene family under salt stress in sweetpotato

et al. 2023

Preprint

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Background The 12-oxo-phytodienoic acid reductase (OPR) enzyme is crucial for the synthesis of jasmonates (JAs) and is involved in the plant stress response. However, the OPR gene family in sweetpotato, an important horticultural crop, remains unidentified. Results In this study, we employed bioinformatics techniques to identify nine IbOPR genes. Phylogenetic analysis revealed that these genes could be divided into Group I and Group II. Synteny analysis indicated that IbOPR evolution was driven by tandem duplication, whole-genome duplication (WGD), and segmental duplication events. The promoter sequences of IbOPRs were found to be associated with stress and hormonal responses. Additionally, we successfully cloned four IbOPRs from "Haida HD7791" and "Haida HD7798" using homologous cloning technology. These sequences were 1203 bp, 1200 bp, 1134 bp, and 1137 bp in length and encoded 400, 399, 377, and 378 amino acids, respectively. The protein sequence similarity between the salt-tolerant variety "Haida HD7791" and the salt-sensitive variety "Haida HD7798" was determined to be 96.75% for IbOPR2, 99.75% for IbOPR3, 92.06% for IbOPR6, and 98.68% for IbOPR7. Phylogenetic analysis categorized IbOPR2 and IbOPR3 proteins into Group II, while IbOPR6 and IbOPR7 proteins belonged to Group I. Subcellular localization experiments showed IbOPR2 present in the peroxisome, while IbOPR3, IbOPR6, and IbOPR7 proteins were found in the cytoplasm and nucleus. Salt stress induction experiments demonstrated that IbOPR2, IbOPR3, and IbOPR7 were significantly upregulated only in 'Haida HD7791' after 6 hours. In contrast, IbOPR6 was induced in 'Haida HD7798' at 6 hours but inhibited in 'Haida HD7791' at later time points (12, 24, 48, and 72 hours), highlighting functional differences in salt stress responses. Conclusions Our findings suggest that IbOPR2 may play a crucial role in sweetpotato's response to salt stress by participating in JAs synthesis. These results provide a foundation for future functional analyses of OPR genes in sweetpotato.

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The jasmonate biosynthesis Gene OsOPR7 can mitigate salinity induced mitochondrial oxidative stress

Cited by 10 publications

References 55 publications

12-Oxophytodienoate Reductase Overexpression Compromises Tolerance to Botrytis cinerea in Hexaploid and Tetraploid Wheat

12-Oxophytodienoate Reductase Overexpression Compromises Tolerance to Botrytis cinerea in Hexaploid and Tetraploid Wheat

Transcriptomic analysis reveals that methyl jasmonate confers salt tolerance in alfalfa by regulating antioxidant activity and ion homeostasis

Genome-wide identification, gene cloning, subcellular location and expression analysis of the OPR gene family under salt stress in sweetpotato

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