The isoquinoline alkaloid sanguinarine which inhibits chaperone activity enhances the production of heat shock proteins in <i>Arabidopsis</i>

Abstract

Sanguinarine is an isoquinoline alkaloid produced by Papaveraceae plants. Because sanguinarine has antimicrobial activity, it is believed to be related to the plants' chemical defense systems. However, its action against plants has not been well understood. A previous study reported that among 12 alkaloids, sanguinarine was the only compound which enhanced heat tolerance in Arabidopsis. Here we performed a promoter assay using a heat shock protein gene (HSP17.6C-CI) of Arabidopsis to assess the induction of heat shock responses by alkaloids. Although sanguinarine induced the heat shock response, the other 11 alkaloids did not. Sanguinarine promoted the production of HSP17.6C-CI protein, but berberine and papaverine, which are isoquinoline alkaloids as well as sanguinarine, did not promote it. It is known that geldanamycin, a small molecule chaperone inhibitor, activates the heat shock response in Arabidopsis. Although sanguinarine inhibited the chaperone activities of wheat germ extract much like geldanamycin, berberine and papaverine influenced the activities very little. These results suggest that sanguinarine may promote the heat shock response by regulating the chaperone activities in the way that geldanamycin does in plants.

Potential use of essential oils to enhance heat tolerance in plants

“…When GDA and sanguinarine were added to Arabidopsis plants, similar dose and timecourse responses were observed in the gene expression and protein accumulation of HSPs [42,47]. GDA and sanguinarine inhibited luciferase folding by wheat (Triticum aestivum L.) germ chaperones at similar efficiencies [47]. This suggests that sanguinarine induces HSR probably via the inhibition of chaperones that hold HSF [48].…”

“…Recently, the benzophenanthridine alkaloid sanguinarine was predicted to have a similar mechanism to GDA. When GDA and sanguinarine were added to Arabidopsis plants, similar dose and timecourse responses were observed in the gene expression and protein accumulation of HSPs [42,47]. GDA and sanguinarine inhibited luciferase folding by wheat (Triticum aestivum L.) germ chaperones at similar efficiencies [47].…”

Composição química, polifenóis totais, atividade antioxidante e citotóxica do extrato etanólico de frutos da Vitex gardneriana Schauer

“…O estudo fitoquímico feito com EEFVG mostrou a presença de alcalóides, apresentando resposta positiva em quatro tubos de ensaio, com a formação de precipitados e mudanças de coloração conforme evidenciado na Tabela 1. A presença de alcalóides pode conferir ao fruto atividade farmacológica como proteção contra radiação UV, proteção contra herbívoros, ação antimicrobiana, antioxidante e antimutagênica (Matsuoka et al, 2016).…”

Diversity in Chemical Structures and Biological Properties of Plant Alkaloids

“…Sanguinarine is a benzophenanthridine alkaloid, belonging to the BIA class of alkaloids extracted from many plant species, such as Sanguinaria canadensis , Chelidonium majus, and Macleaya cordata [ 83 ]. The BIA alkaloid ( S )-Scoulerine undergoes several modification reactions to generate a series of alkaloids possessing diverse biological properties.…”