Biomembranes 1971
DOI: 10.1007/978-1-4684-3327-2_4
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The Isolation of the Surface Membranes of Animal Cells: A Survey

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Cited by 18 publications
(5 citation statements)
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“…Better yields and membranes of comparable or higher purity (~ 14-fold) were obtained by simplifying the original Warren procedure (FMA II). FMA membranes prepared by either procedure displayed fibrillar material and many ribosome-like particles adsorbed to the cytoplasmic face, as reported by others using different tissue culture cells (28,63). These particles did not appear to be attached to the plasma membrane in the intact cell (20) and exposure of the isolated membranes to a high salt concentration (0.5 M KC1) released most of them (1).…”
Section: Membrane-enriched Preparationssupporting
confidence: 57%
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“…Better yields and membranes of comparable or higher purity (~ 14-fold) were obtained by simplifying the original Warren procedure (FMA II). FMA membranes prepared by either procedure displayed fibrillar material and many ribosome-like particles adsorbed to the cytoplasmic face, as reported by others using different tissue culture cells (28,63). These particles did not appear to be attached to the plasma membrane in the intact cell (20) and exposure of the isolated membranes to a high salt concentration (0.5 M KC1) released most of them (1).…”
Section: Membrane-enriched Preparationssupporting
confidence: 57%
“…Methods published by Warren and colleagues (63,64) employing Zn § fluorescein mercuric acetate (FMA), and Tris-HC1 at pH 7.4 and 8.0 as membrane-"stabilizing" agents were followed and found to be time consuming and quite variable in yield and purification, as assessed by TCA-insolubte ~2~I per milligram protein.…”
Section: Isolation Of L Cell Plasma Membranementioning
confidence: 99%
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“…The procedure was monitored in the phase contrast microscope, and after gentle homogenization in a Dounce with a loose-fitting pestle the membrane can be observed coming off the cell. Sucrose gradients were used to separate the membranes from cell particulates, nuclei, and remaining whole cells as described (Warren and Glick, 1969). The surface membranes prepared by this method were identifiable morphologically 30% sucrose and visualized in a Zeiss phase contrast microscope.…”
Section: Preparation Of Surface Membranesmentioning
confidence: 99%
“…Πρόσθετα τά μικρού μήκους αλυσίδας FA έχουν μικρότερο εμβαδόν καί ευκολία γιά περιστροφική κίνηση. 13 Στίς επιφανειακές 1 δμως μεμβράνες πού περιέχουν μιά ποικιλία λιπιδικών τάξεων καί σέ κά θε τάξη αντιστοιχεί ένα μεγάλο καί συναρπαστικό φάσμα FA,χρειάστηκε νά γίνουν μερικές χρή σιμες γενικεύσεις. "Ετσι γιά κάθε τάξη μεμβρανικοΰ λιπιδίου καί γιά κάθε ϋερμοκρασία ή μεμβρανική ρευστότητα είναι μεγαλύτερη γιά μικρό μήκος ύδρογονανθρακικής αλυσίδας, καί γιά περισσότερο ακόρεστα άκυλο-παράγωγα.…”
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