The isolation of structural genes from libraries of eucaryotic DNA

Maniatis, Tom; Hardison, Ross C.; Lacy, Elizabeth; Lauer, Joyce; O'Connell, Catherine D.; Quon, Diana; Sim, Gek-Kee; Efstratiadis, Argiris

doi:10.1016/0092-8674(78)90036-3

Cited by 1,835 publications

(788 citation statements)

References 47 publications

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“…This is not unexpected because the /3-globin gene is separated from the enhancer by a full-length copy of a bacterial plasmid at its 5' end and by the strong IE promoter of HCMV at its 3' end. It has been shown that (Maniatis et al, 1978) in clone p/3X14 (a gift from J. Banerji), which is a derivative of p~GX containing a Hind III linker at the single Xba I site. The Hind III C-fragments of recombinant viruses C2 and C4 were inserted at this Hind Ill site in either orientation as indicated at the top.…”

Section: The Enhancer Effect Is Obscured By Hcmv Promoter Sequencesmentioning

confidence: 99%

A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus

Boshart

Weber

Jahn

et al. 1985

Cell

1,094

648

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SummaryA strong transcription enhancer was identified in the genomic DNA (235 kb) of human cytomegalovirus (HCMV), a ubiquitous and severe pathogen of the herpesvirus group. Cotransfection of enhancerless SV40 DNA with randomly fragmented HCMV DNA yielded two SV40-HCMV recombinant viruses that had incorporated overlapping segments of HCMV DNA to substitute for the missing SV40 enhancer. Within HCMV, these enhancer sequences are located upstream of the transcription initiation site of the major immediate-early gene, between nucleotides -118 and -524. Deletion studies with the HCMV enhancer, which harbors a variety of repeated sequence motifs, show that different subsets of this enhancer can substitute for the SV40 enhancer. The HCMV enhancer, which seems to have little cell type or species preference, is severalfold more active than the SV40 enhancer. It is the strongest enhancer we have analyzed so far, a property that makes it a useful component of eukaryotic expression vectors.

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Section: The Enhancer Effect Is Obscured By Hcmv Promoter Sequencesmentioning

confidence: 99%

A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus

Boshart

Weber

Jahn

et al. 1985

Cell

1,094

648

View full text Add to dashboard Cite

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“…This DNA fragment was then used to screen a Drosophila genomic library in bacteriophage vector Charon 4 (Maniatis et al, 1978;kindly provided by Ben Zion Shilo, Weizmann Institute, Rehovot, Israel). Approximately 60 000 phage plaques were plated at density of 15 000 plaques per plate (150 mm).…”

Section: Isolation Of a Clone Encoding Dap-2mentioning

confidence: 99%

Cloning and characterization of the Drosophila homologue of the AP-2 transcription factor

et al. 1998

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The AP-2 family of transcription factors (AP-2a, AP-2b and AP-2g) is temporally and spatially regulated in mammals and has also been implicated in oncogenesis. Here we report the isolation of genomic and cDNA clones encoding the Drosophila homologue of AP-2, designated DAP-2. The predicted amino acid sequence exhibits 42 ± 45% overall identity with the vertebrate AP-2 proteins. A sequence of 107 amino acids within the DNA binding and dimerization domain of the vertebrate AP-2 proteins is highly conserved (90 ± 92%) with the DAP-2 homologue. An in vitro translation product of DAP-2 cDNA binds speci®cally to AP-2 consensus binding sites. DAP-2 was also shown to be functionally conserved in vivo because transient transfection of a DAP-2 expression plasmid activated transcription through AP-2 binding sites in both mammalian and Drosophila cell lines. DAP-2 is expressed during early embryogenesis and DAP-2 transcripts are also detected in the adult. Whole-mount in situ hybridizations demonstrated that DAP-2 is expressed initially at stage 9 of Drosophila embryonic development and that DAP-2 transcripts are detected in regions of the brain, eyeantennal disc, optical lobe, antenno-maxillary complex, and in a subset of cells of the ventral nerve cord. The cloning of DAP-2 and the identi®cation of the DAP-2 expression pattern during embryogenesis provides a starting point to address the function of AP-2 during di erentiation and development in a well understood model system.

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“…A phage genomic library of D. melanogaster (Maniatis et al 1978; a gift of G. Guild) was screened with the probe under low stringency conditions (3 x SSC, 0.1% SDS, 1 x blocking reagent (Boehringer Mannheim) at 43°C overnight; filters were washed with 2 x SSC, 0.5% SDS at 48°C). Fifteen independcnt positives were isolated, and plate lysates were used to prepare DNAs from them.…”

Section: Cloning Of Rpb Genesmentioning

confidence: 99%

Runt domain partner proteins enhance DNA binding and transcriptional repression in cultured Drosophila cells

et al. 1996

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Background The Drosophila gene runt plays multiple roles during embryogenesis, including one as a pairrule class segmentation gene. The runt protein (Runt) contains an evolutionarily conserved domain (the Runt domain) that is found in several mammalian proteins including the human protein AMLI, which is involved in many chromosome translocations associated with leukaemia. Specific DNA binding activity of a mammalian Runt domain is enhanced

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The isolation of structural genes from libraries of eucaryotic DNA

Cited by 1,835 publications

References 47 publications

A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus

A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus

Cloning and characterization of the Drosophila homologue of the AP-2 transcription factor

Runt domain partner proteins enhance DNA binding and transcriptional repression in cultured Drosophila cells

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