The Isolation of Strict Anaerobes: the Use of an Anaerobic Cabinet Compared with a Conventional Procedure

Watt, B.; Collee, J. G.; Brown, Robert L.

doi:10.1099/00222615-7-3-315

Cited by 20 publications

(12 citation statements)

References 20 publications

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“…An anaerobic cabinet (Aranki et a/., 1969; Aranki and Freter, 1972) was not used because Watt, Collee and Brown (1974) had shown that manipulation in a cabinet had no advantage for the recovery of anaerobes from faeces over prompt conventional handling at the bench with a well-controlled and standardised jar technique and freshly prepared or pre-reduced media. Rosenblatt, Fallon and Finegold (1973) and Starr (1974) also found that roll tubes (Hungate, 1950) had no advantage over conventional bench methods.…”

Section: Discussionmentioning

confidence: 99%

The Isolation and Identification of Bacteroides Spp. From the Normal Human Faecal Flora

Duerden¹

1980

Journal of Medical Microbiology

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GRAM-NEGATIVE anaerobic bacilli are a major component of the normal flora of human faeces. In quantitative studies, Bacteroides spp. have been isolated in counts of 10" organisms/g of faeces (Hill et al., 1971;Finegold, Attebury and Sutter, 1974;Finegold, 1977). Drasar (1 974) isolated Bacteroides in greater numbers from subjects from developed countries whereas gram-positive anaerobes were predominant in the faeces of subjects from developing countries.Investigators who have identified faecal Bacteroides have found that the B. fragilis group are the dominant species. A previous taxonomic scheme (Holdeman and Moore, 1974a) assigned all the bile-and penicillin-resistant faecal strains to the single species B. fragilis, with five subspecies; when detailed identification was undertaken the commonest faecal subspecies were ss. vulgatus and ss. thetaiotaomicron (Werner, 1974;Moore and Holdeman, 1975;Finegold et al., 1975). However, DNA-homology studies have shown that each subspecies is unique and should be assigned species status (Cato and Johnson, 1976) and several new species have been described to accommodate strains with different properties within the B. fragilis group (Holdeman and Moore, 19746;Werner, Rintelen and Kunstek-Santos, 1975). Many other Bacteroides and Fusobacterium spp. have been found inconsistently in small numbers in faeces. This may reflect the problems of isolation, identification and classification of these organisms. B. asaccharolyticus (B. melaninogenicus ss. asaccharolyticus; Finegold and Barnes, 1977) has been isolated regularly from faeces (Werner, Pulverer and Reichertz, 1971) but in much smaller numbers than the B. fragilis group.The present study was undertaken to assess the contribution of the different species and subspecies of Bacteroides to the normal faecal flora as part of the evaluation of a scheme for the identification of gram-negative anaerobic bacilli by conventional bacteriological tests (Duerden et al., 1980).

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Section: Discussionmentioning

confidence: 99%

The Isolation and Identification of Bacteroides Spp. From the Normal Human Faecal Flora

Duerden¹

1980

Journal of Medical Microbiology

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“…Improvements in anaerobic techniques (Collee, Rutter & Watt, 1971;Holdeman & Moore, 1973;Watt, 1973;Watt, Hoare & Collee, 1973;Watt, Collee & Brown, 1974) have provided laboratories with reliable methods for the isolation of bacteroides organisms, but precise identification is rarely attempted in the routine laboratory. The classification of gram-negative anaerobic bacilli has been confused, and in most laboratories methods have not been available for accurate identification.…”

Section: Introductionmentioning

confidence: 99%

The identification of gram-negative anaerobic bacilli isolated from clinical infections

Duerden

1980

J. Hyg.

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SUMMARYGram-negative anaerobic bacilli isolated from specimens submitted to the routine diagnostic bacteriology laboratory and regarded as significant pathogens were identified by conventional bacteriological tests; 399 strains isolated from 356 specimens submitted from 332 patients were studied and most were readily identified by the results of a combined set of morphological, biochemical, tolerance and antibiotic disk resistance tests. B. fragilis has particular pathogenic potential and was the commonest species isolated, accounting for > 50 % of strains. The next commonest was B. asaccharolyticus with 55 strains, and 16 other species or groups were represented by smaller numbers. Many (68 %) were from infections related to the gastro-intestinal tract, but there were significant numbers from infections of the male and female genito-urinary tracts, the head, neck and central nervous system and from a variety of soft tissue infections. Most infections were mixed, and a pure culture of a Bacteroides sp. was obtained from only 26 % of infections; two or more strains of Bacteroides were recovered from 55 infections. The specific identification of Bacteroides may help the bacteriologist to judge the significance of laboratory findings, influence the patient's management and prognosis and help determine the source of infection.

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“…Consequently a simple method was sought that would allow reliable isolation of anaerobic bacteria and that would be acceptable as a routine procedure in a dental hospital. Watt, Collee and Brown (1974) have shown that careful bench handling of specimens of faeces yielded anaerobes in numbers similar to those obtained by manipulation in an anaerobic cabinet. Rosenblatt, Fallon and Finegold (1973) and Starr (1974) have also found no advantage in the use of roll tubes over conventional bench methods.…”

Section: S E V E R a Lmentioning

confidence: 59%

A method for the isolation of Bacteroides melaninogenicus from the human mouth

Holbrook

Ogston

Ross

1978

Journal of Medical Microbiology

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The Isolation of Strict Anaerobes: the Use of an Anaerobic Cabinet Compared with a Conventional Procedure

Cited by 20 publications

References 20 publications

The Isolation and Identification of Bacteroides Spp. From the Normal Human Faecal Flora

The Isolation and Identification of Bacteroides Spp. From the Normal Human Faecal Flora

The identification of gram-negative anaerobic bacilli isolated from clinical infections

A method for the isolation of Bacteroides melaninogenicus from the human mouth

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