The isolation of lytic enzymes from Cytophaga and their application to the rupture of yeast cells

Abstract

SummaryYeast-lytic enzymes have been isolated on a pilot scale from Cytophaga species by precipitation and the light, enzyme-rich solid phase recovered by liquid-liquid separation. The enzyme complex was immobilized to soluble polymeric carbohydrates and the effectiveness of the free and immobilized enzyme for protein release and cell debris dissolution has been assessed.

Microbial Protein Production from Agro-industrial Wastes as Food and Feed

“…It is possible to use two or more methods for cell disruptions. Combined mechanical and enzymatic degradation of yeast cell wall was tested [4].…”

Proteolytic activity of a yeast cell wall lytic Arthrobacter species

“…Effective lysis of cells were described with enzymes prepared from Arthrobacter luteus (Kitamura 1982), Oerskovia xanthineolytica (Scott and Schekman 1980) and Cytophaga sp. (Asenjo and Dunnill 1981). Klinger and Röhr (1972) isolated from soil samples a bacterium capable of lysing yeast cell walls, which was identified as Arthrobacter species.…”

The lysis of Gram-negative Alcaligenes eutrophus by enzymes from Cytophaga

“…However, pilot plant studies on the production of Iytic el~zymes by Cyfophaga suggest an emerging alternative (Asenjo & Dunnill 1981, Andrews & Asenjo 1987. The lytic enzyme system of Cytophaga sp has been extensively studied for the lysis of yeast cells (Asenjo & Dunnill 1981, Hunter & Asenjo 1988, Liu et al 1988. High enzyme activity and the absence of product inhibition was reported for the lysis of the Gram-positive bacteria Bacillus and Corynebacterium (LeCorre et al 1985).…”