The Isolation of Lumpy Skin Disease Virus and Bovine Herpesvirus- from Cattle in Egypt

House, James A.; Wilson, T. M.; Nakashly, Sinan El; Karim, Ikram A.; Ismail, I.; Danaf, Nabil El; Moussa, Ayman S.; Ayoub, N. N.

doi:10.1177/104063879000200205

Cited by 91 publications

(93 citation statements)

References 3 publications

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“…Thrombocytopenia occurred in all infected cattle and was mainly attributed to the shortening of the platelet life span. This phenomenon is typically caused by excessive platelet consumption due to systemic vasculitides, which were widespread in our study due to the tropism of LSDV to endothelial cells (House et al 1990;Radostitis et al 2000). With respect to biochemical results, total protein and globulin levels were elevated in LSDV-infected cattle; however, dehydration may elevate the total protein level, also albumin levels were reduced, likely due to increased protein catabolism or decreased protein synthesis as well as hepatic damage (Hassan et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Hematological, biochemical and oxidative stress studies of lumpy skin disease virus infection in cattle

El-Mandrawy

Alam

2018

Journal of Applied Animal Research

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Lumpy skin disease (LSD) is an important disease of cattle. This study was conducted to determine alterations in hematological, biochemical and oxidative stress markers in cattle that have been naturally infected with LSD virus (LSDV). Blood samples and skin nodular lesions were collected from clinically infected, recovered and healthy animals. Quantitative real-time PCR was used to screen for Capripoxvirus DNA in samples from clinically infected animals. Hematological, biochemical and oxidative stress markers were measured. LSDV nuclic acids were detected in the collected samples using PCR. Hematological results revealed erythrocytosis, thrombocytopenia and leukopenia in infected cattle. Biochemical analyses showed that total protein and globulin levels were significantly elevated, while albumin and glucose were significantly reduced in these cattle. Aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), creatine phosphokinase (CPK), and creatinine levels were markedly elevated. Moreover, serum levels of reduced glutathione (GSH) were markedly lowered, whereas lipid peroxidation (MDA) and inflammatory cytokines (IL-4 and TNF-α) were elevated. Recovered cattle exhibited significant amelioration of the alterations resulting from LSDV infection. The results of this study suggest that LSDV infection induces changes in hematological and biochemical parameters and stimulates oxidative stress; these findings may be helpful for choosing a good strategy for rapidly detecting and diagnosing LSDV infection. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Hematological, biochemical and oxidative stress studies of lumpy skin disease virus infection in cattle

El-Mandrawy

Alam

2018

Journal of Applied Animal Research

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“…The first report of LSD outside Africa was from Kuwait in 1986-1988 [1] , followed by Israel in 1989 [30] . In Egypt, the LSD was first appeared in the Suez Governorate after cattle were imported from Somalia followed by the Ismailyia Governorate in 1988 [18] and two disease outbreaks were reported in 2005 and 2006 [26,36] . There were 2 outbreaks of LSD in Israel during 2006 and 2007 and it is believed be transmitted by yet an unconfirmed insect vector from Egypt [31] .…”

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confidence: 99%

Sequence analysis of attachment gene of lumpy skin disease and sheep poxviruses

El-Kenawy

El‐Tholoth

2010

Virol. Sin.

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In Egypt, protection of cattle against lumpy skin disease (LSD) was carried out using a sheep poxvirus (Kenyan strain) vaccination strategy. In the present study 15 skin nodules from LSD suspected cows and 5 scab samples from sheep pox (SP) suspected sheep were collected. Hyperimmune rabbit sera to Lumpy skin disease virus (LSDV)/Ismailyia88 strain and sheep pox virus (SPV)/ Kenyan vaccinal strain were prepared. The causative agent in the collected samples was identified using immunoflourescence (IF) and immunoperoxidase techniques. Of the 15 skin nodules suspected of LSD, 10 showed a positive reaction and 3 out of 5 skin scabs suspected of sheeppox were found to be positive. An antigenic correlation between field skin isolate of LSDV, tissue culture adapted LSDV/Ismailyia88 strain, field skin isolate of SPV and SPV/Kenyan vaccinal strain was studied using prepared hyperimmune sera. Also, nucleotide sequence of the PCR amplified attachment gene fragments of field skin isolate of LSDV, tissue culture adapted LSDV/Ismailyia88 strain, field skin isolate of SPV and SPV /Kenyan vaccinal strain were compared. The results revealed that the four used viruses were antigenically identical. Sequence analysis indicated that field skin LSDV isolate is more related to tissue culture adapted LSDV/Ismailyia88 strain than to vaccinal SPV/ Kenyan strain and the skin isolate of SPV is more closely related to field skin isolate of LSDV than to SPV/Kenyan vaccinal strain. Thus, further study should be applied on the advantage of a LSD vaccine prepared from LSDV in protection of cattle against LSD compared to the commonly used sheep pox vaccine.

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“…In addition, the disease disrupts the trade in cattle and their products from LSD endemic countries [3]. LSD was initially restricted to countries in sub-Saharan Africa, although, there were unconfirmed reports of the disease in cattle in Oman and Kuwait [4,5]. Since 2000, LSD outbreaks have been reported across the Middle East and it is highly likely the disease will become endemic at least in parts of the Region.…”

Section: Introductionmentioning

confidence: 99%

Lumpy Skin Disease

Abdulqa¹,

Rahman²,

Dyary³

et al. 2016

Reproductive Immunol Open Acc

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Lumpy skin disease (LSD) is an infectious, eruptive, occasionally fatal disease of cattle caused by a virus of the family Poxviridae (genus Capripox), which is sometimes also termed as Neethling virus. LSD does not have a high fatality rate, usually less than 10%. LSD has an economical importance because of permanent damage to hides, the prolonged debilitating effect especially in severely affected animals with consequent losses resulting from reduced weight gain, temporary or permanent cessation of milk production as a result of mastitis, temporary or permanent infertility or even sterility in bulls as a consequence of orchitis, and abortion in approximately 10% of infected pregnant cows.

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The Isolation of Lumpy Skin Disease Virus and Bovine Herpesvirus- from Cattle in Egypt

Cited by 91 publications

References 3 publications

Hematological, biochemical and oxidative stress studies of lumpy skin disease virus infection in cattle

Hematological, biochemical and oxidative stress studies of lumpy skin disease virus infection in cattle

Sequence analysis of attachment gene of lumpy skin disease and sheep poxviruses

Lumpy Skin Disease

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