The isolation of <i>Frankia</i> from nodules of <i>Casuarina</i>

Diem, Hoang Gia; Dommergues, Yvon

doi:10.1139/b83-313

Cited by 68 publications

(35 citation statements)

References 5 publications

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“…Nodule lobes were superficially sterilized in a 2 % (w/v) aqueous solution of osmium tetroxide (Lalonde et al, 1481) for 3 min. Nodule lobes were then thoroughly rinsed in sterile water, chopped into small pieces and transferred into a double-layer agar medium as recommended by Diem & Dommergues (1983). After 4 weeks, Frankia colonies grew out of nodule pieces and exhibited typical hyphae and sporangia.…”

Section: Methodsmentioning

confidence: 99%

Differentiation of Frankia strains by their electrophoretic patterns of intracellular esterases and aminopeptidases

Girgis

Schwencke

1993

Journal of General Microbiology

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The closely related Frankia strains BR, S21 and Thr, isolated from the genus Casuauinu, All02 and Dec from the genus Allocnsuauina, and GSO from the genus Gymnostoma, could be distinguished by their esterase zymograms after ultra-low gelling point agarose-polyacrylamide electrophoresis. The kanamycin-resistant derivatives S21-kR and BR-kR could also be differentiated from their kanamycin-sensitive parental strains. Different patterns of intracellular esterases could be obtained by using P-naphthyl propionate or 3-iadoxyl acetate as substrates. The substrate 5-bromo-4-chloro-3-indoxyl acetate proved particularly useful, revealing a variety of esterases from Gymnostoma isolates. Zymograms of aminopeptidases from all strains were found to be quite similar. Nevertheless, they allowed differentiation of Frankia strains BR and S21 from their kanamycin-resistant derivatives. Aminopeptidase and esterase zymograms obtained from Gymnostornu isolates were markedIy different from all others. Zymograms of esterases and aminopeptidases may prove useful for the identification of some other Frankia strains.

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Section: Methodsmentioning

confidence: 99%

Differentiation of Frankia strains by their electrophoretic patterns of intracellular esterases and aminopeptidases

Girgis

Schwencke

1993

Journal of General Microbiology

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“…Casuarina nodule lobes were superficially sterilized in a 3% (w/v) aqueous solution of osmium tetraoxide for 4 min to eliminate contaminants harboured in the outer layer of the nodule (Lalonde et al, 1981). Nodule lobes were then thoroughly rinsed in sterile water, chopped into small pieces and transferred into a double layer agar medium as recommended by Diem & Dommergues (1983). After 10-15 d, Frankia colonies grew out of nodule pieces and exhibited typical hyphae and sporangia.…”

Section: Methodsmentioning

confidence: 99%

Age-dependent changes in extracellular proteins, aminopeptidase and proteinase activities in Frankia isolate BR

Muller

Benoist²,

Diem³

et al. 1991

Journal of General Microbiology

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To investigate protein secretion by the nitrogen-fixing actinomycete Frunkiu isolate BR, we designed a rapid DEAE adsorption, salt elution and Biogel P6DG desalination method to concentrate protein from the growth medium. Secreted proteins reached a maximum concentration (5.6 mg I-') in the medium at growth arrest. Analysis by SDS-PAGE detected up to 63 extracellular polypeptides when Frunkiu cells were grown under stirred conditions in BAP medium supplemented with phosphatidylcholine and MES buffer and 65 proteins in stirred BAP media alone. The pattern of extracellular polypeptides changed during growth. Several extracellular proteolytic activities were detected and compared with intracellular ones. The substrate specificity of the extracellular and intracellular aminopeptidase activities were the same. Also, the electrophoretic migration patterns of secreted and intracellular aminopeptidases could not be distinguished. Secretion of the proline-specific aminopeptidase FAP 3.BR appeared to be the only one that arrested at the end of the exponential phase of growth. At least 15 proteinases (PF) were secreted: 10 had the same electrophoretic mobility as their intracellular counterparts after SDS-gelatine-PAGE while five (PF-39.5, PF-38.5, PF-36.5, PF-25.5 and PF-20.5 kDa) had a different electrophoretic mobility and, therefore, appeared to be exclusively extracellular. At least seven extracellular proteinases appeared to increase coordinately in activity shortly before growth arrest.

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“…(Diem & Dommergues, 1983) and reported as infective and effective on the genus Casuarina in field conditions was chosen. In order to obtain Frankia culture only composed of young vegetative hyphae, the strain has been subcultured three times for eight to ten days.…”

Section: Preparation Of Bacterial and Frankia Inoculummentioning

confidence: 99%

“…Casuarinaceae is a tree family able to develop a nitrogen-fixing symbiosis with the actinomycete Frankia (Diem & Dommergues, 1983). This particular symbiosis called "actinorhizae" enables species of Casuarinaceae to grow in adverse ecological conditions such as land reclamation, reforestation and soil stabilization (Richards eta/., 2002).…”

Section: Introductionmentioning

confidence: 99%

Helper bacteria associated with Casuarina cunninghamiana-Frankia symbiosis: selection of isolates for their effects on plant growth in axenic conditions

Echbab¹,

Prin

Ducousso

et al. 2004

Acta Botanica Gallica

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Abstract.-The formation of Casuarina actinorhizae in axenic conditions remains a challenge hindering research program. To explore the putative role of helper bacteria favouring actinorhizae formation, 50 bacteria were tested in an original selection scheme for their effects on C. cunninghamiana growth in the presence of Frankia. Two strains FAB1 and FAB2 were identified as having a significant promoting effect on C. cunninghamiana growth in the presence of Frankia. Actinorhizae formation was never observed in these strictly axenic conditions. The comparison of 165 rDNA sequences shows similarity of 99% for FAB1 with Bacillus sp. and of 99% for FAB2 with Ochrobactrum sp.Key words : axenic culture -Casuarina -Frankia -helper bacteria -symbiosis.

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The isolation of Frankia from nodules of Casuarina

Cited by 68 publications

References 5 publications

Differentiation of Frankia strains by their electrophoretic patterns of intracellular esterases and aminopeptidases

Differentiation of Frankia strains by their electrophoretic patterns of intracellular esterases and aminopeptidases

Age-dependent changes in extracellular proteins, aminopeptidase and proteinase activities in Frankia isolate BR

Helper bacteria associated with Casuarina cunninghamiana-Frankia symbiosis: selection of isolates for their effects on plant growth in axenic conditions

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