THE ISOLATION OF CILIARY BASAL BODIES (KINETOSOMES) FROM <i>TETRAHYMENA PYRIFORMIS </i>

Argetsinger, Joan E.

doi:10.1083/jcb.24.1.154

Cited by 49 publications

(24 citation statements)

References 4 publications

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“…However, the fact that only the surface is covered with centriole material greatly reduces the significance of the negative Feulgen reaction. D N A has been reported in basal bodies of the ciliate Tetrahymena on the basis of chemical studies of isolated material (33)(34)(35). More recently, Randall and Disbrey (36) describe acridine orange staining of basal bodies which they believe is due to the presence of trace amounts of DNA; they also obtain thymidine incorporation into the basal bodies.…”

Section: From the Limited Observations Availablementioning

confidence: 99%

Centriole Replication

Mizukami

Gall

1966

The Journal of Cell Biology

143

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Sperm formation was studied in the fern, Marsilea, and the cycad, Zamia, with particular cmphasis on thc ccntrioles. In Marsilea, the maturc spcrm posscsses over 100 flagella, the basal bodies of which have the typical cylindrical structure of centriolcs. Earlier obscrvations by light microscopy suggcsted that these centrioles arise by fragmentation of a body known as the blepharoplast. In the youngest spermatids the blepharoplast is a hollow sphere approximately 0.8 tt in diameter. Its wall consists of closely packed immature ccntriolcs, or proccntriolcs. Thc procentrioles arc short cylinders which progressively lengthen during differentiation of the spermatid. At the same time thcy migrate to the surface of thc ccll, where each of thcm puts out a i]agcllum. A blepharoplast is found at cach pole of thc spindle during the last antheridial mitosis, and two blcpharoplasts are found in the cytoplasm before this mitosis. Blepharoplasts arc also found in the prcccding cell generation, but their ultimatc origin is obscure. Before thc last mitosis the blepharoplasts are solid, consisting of a clustcr of radially arranged tubulcs which bear some structural similarity to centrioles. In Zamia, similar stages are found during sperm formation, although here thc number of flagella on each sperm is close to 20,000 and thc blepharoplast measures about l0 tt in diameter. These observations arc discussed in relation to theories of ccntriolc replication.

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Section: From the Limited Observations Availablementioning

confidence: 99%

Centriole Replication

Mizukami

Gall

1966

The Journal of Cell Biology

143

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“…Several investigators have tried to purify individual cortical structures, such as ciliary basal bodies and associated fibers. Their isolation procedures have not allowed unequivocal conclusions concerning nucleic acids (2,13,25) or proteins (23) present in the cortex, due to difficulties in eliminating impurities. Procedures for isolating whole pellicles (4,7,12,14,17,29) or oral apparatuses (20, 31), devoid of cilia, have been more successful.…”

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confidence: 99%

Isolation and Identification of Specific Cortical Proteins in Tetrahymena pyriformis strain GL*^†

Vaudaux

1976

The Journal of Protozoology

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Pellicles of the ciliate Tetrahymena pyriformis strain GL (phenoset A) were isolated by a new procedure. Oral apparatuses also purified by a modification of a pervious method. Both preparations were characterized by electron microsocpy. Proteins of the isolates were separated by analytical SDD polyacrylamide gel electrophoresis. The isolated pellicles, which included oral apparatuses, contained only 6 major proteins (gel bands), designated A through F. Bands A, B, and C, were found in the pellicle fraction, but not in the oral apparatus fraction. Therefore, these proteins are believed to be present in the somatic cortex of Tetrahymena. Bands D and E were greatly enriched in the oral apparatus fraction; these proteins are therefore believed to be present primarily in the oral apparatus. Band F, identified as tubulin, was present in both preparations. Molecular weight determinations and some selective solubilization experiments are also presented.

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“…In addition to differences among strains, a medium relatively low in the concentration of nutrients, a culture LTHOUGH a number of flagellates, amoebae and sporo-A zoa have been preserved by freezing, ciliated protozoa have rarely survived following freezing. Efimoff ( 4 ) subjected Paramecium caudatum, Colpidium colpoda and Spirostomum ambiguum to freezing under carefully controlled conditions. Only P .…”

Section: Survival Of Tetrahymena Pyrif Ormis and Paramecium Aurelia Fmentioning

confidence: 99%

Amino Acid Activation in Subcellular Fractions of *Tetrahymena pyriformis**

Rosenbaum

Holz

1966

The Journal of Protozoology

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SYNOPSIS. The presence of amino acid activating enzymes was demonstrated in the ciliated protozoan Tetrahymena pyriformis. By employing a sensitive hydroxamate assay procedure, the activation of L‐valine was assayed in various subcellular fractions of the ciliate, and some characteristics of the enzyme activity in the most active fraction were determined. Most of the activity resided in pH 5 fractions isolated from high speed supernatants of ciliates disrupted by various physical and chemical methods. No activity could be demonstrated in isolated cilia, in pellicles with attached kinetosomes, in microsomes or in macronuclei, providing these organelles were thoroughly washed. A washed mitochondrial preparation isolated by the Mager and Lipmann procedure activated L‐valine; mitochondria isolated by the procedure of Hogg and Kornberg did not. The pH 5 fraction isolated from the 102,000 X g supernatant of digitonin‐lysed ciliates was stable for several weeks when stored in 0.1 M Tris buffer, pH 7.6 at – 25 C. The activity of this fraction with respect to L‐valine activation was dependent on the presence of ATP1 and magnesium in the reaction mixture. The optimal concentrations of these components and of L‐valine and hydroxylamine were determined, and the linearity of activity with time and enzyme concentration was demonstrated. Valine activation was not modified by dialysis of the pH 5 fraction, or treatment with RNase, or the addition of boiled pH 5 fraction.

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THE ISOLATION OF CILIARY BASAL BODIES (KINETOSOMES) FROM TETRAHYMENA PYRIFORMIS

Cited by 49 publications

References 4 publications

Centriole Replication

Centriole Replication

Isolation and Identification of Specific Cortical Proteins in Tetrahymena pyriformis strain GL*^†

Amino Acid Activation in Subcellular Fractions of *Tetrahymena pyriformis**

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THE ISOLATION OF CILIARY BASAL BODIES (KINETOSOMES) FROM TETRAHYMENA PYRIFORMIS

Cited by 49 publications

References 4 publications

Centriole Replication

Centriole Replication

Isolation and Identification of Specific Cortical Proteins in Tetrahymena pyriformis strain GL*†

Amino Acid Activation in Subcellular Fractions of Tetrahymena pyriformis*

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Product

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Isolation and Identification of Specific Cortical Proteins in Tetrahymena pyriformis strain GL*^†

Amino Acid Activation in Subcellular Fractions of *Tetrahymena pyriformis**