The Isolation of 4-Thiouridylic Acid from the Soluble Ribonucleic Acid of Escherichia coli

Lipsett, Marie N.

doi:10.1016/s0021-9258(18)97137-1

Cited by 234 publications

(27 citation statements)

References 16 publications

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“…of in vitro and in vivo labeled [3SS]tRNA were chromatographed on DEAE-cellulose according to the method of Lipsett (1965). This technique successfully separated s4UMP from the other nucleotides as shown in Figure 6A for in vivo labeled [35S]tRNA.…”

Section: Resultsmentioning

confidence: 99%

“…The digest was carefully adjusted to pH 8 using Dowex 50 (H+) before chromatography. DEAE-cellulose chromatography was carried out by the method of Lipsett (1965), and a s4UMP was prepared from a digest of E. coli B tRNA using this technique. The method of Carbon (1965) was used for Dowex 1 formate chromatography, and was used to isolate s2CMP from in vivo labeled [3SS]tRNA.…”

Section: Methodsmentioning

confidence: 99%

“…T -transfer ribonucleic acid (tRNA) from Escherichia coli has been shown to contain four thionucleotides: s4U4 (Lipsett, 1965), s2C, mnm5s2U (Carbon et al, 1968), and ms2i6A (Burrows et al, 1968). In vivo studies showed that the sulfur portion of these bases was derived from cysteine (Peterkofsky and Lipsett, 1965).…”

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confidence: 99%

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Sulfur-deficient transfer ribonucleic acid. Natural substrate for ribonucleic acid sulfurtransferase from Escherichia coli

Harris¹,

Titchener²

1971

Biochemistry

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Sulfur-deficient tRNA, isolated after cysteine starvation of Escherichia coli HfrC, RCre', met-, cys-, A, was used as the substrate for the crude mixture of E. coli RNA sulfurtransferases. This tRNA accepted nearly four times more sulfur in vitro than normal tRNA. Sulfur incorporation was inversely proportional to the 4-thiouridine content. The optimal concentrations of all of the required cofactors and substrates, ATP, Mg2+, cysteine, and tRNA were determined. The apparent K , for cysteine was 2.6 X M and that for sulfur-deficient tRNA, 2.7 x M. Chromatography of

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Sulfur-deficient transfer ribonucleic acid. Natural substrate for ribonucleic acid sulfurtransferase from Escherichia coli

Harris¹,

Titchener²

1971

Biochemistry

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“…For each geometry, the Dyson norms for all applicable pairs of states were computed using the molecular orbital coefficients and CI vectors from the corresponding quantumchemistry calculations. Spectra were calculated as a sum over Gaussians centered at the excitation energies and height proportional to W in equation (2). A full width at half maximum of 0.2 eV was chosen for the purpose of obtaining a smoothed spectrum from the calculated number of geometries.…”

Section: B Computational Detailsmentioning

confidence: 99%

Photoelectron spectra of 2-thiouracil, 4-thiouracil, and 2,4-dithiouracil

Ruckenbauer

Mai

Marquetand

et al. 2016

The Journal of Chemical Physics

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Ground-and excited-state UV photoelectron spectra of thiouracils (2-thiouracil, 4-thiouracil and 2,4-dithiouracil) have been simulated using multireference configuration interaction calculations and Dyson norms as measure for the photoionization intensity. Except for a constant shift, the calculated spectrum of 2-thiouracil agrees very well with experiment, while no experimental spectra are available for the two other compounds. For all three molecules, the photoelectron spectra show distinct bands due to ionization of the sulphur and oxygen lone pairs and the pyrimidine π system. The excited-state photoelectron spectra of 2-thiouracil show bands at much lower energies than in the ground state spectrum, allowing to monitor the excited-state population in time-resolved UV photoelectron spectroscopy (TRPES) experiments. However, the results also reveal that single-photon ionization probe schemes alone will not allow monitoring all photodynamic processes existing in 2-thiouracil. Especially, due to overlapping bands of singlet and triplet states the clear observation of intersystem crossing will be hampered.

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“…The thione unit, which is naturally present in Escherichia coli tRNAs, 17 has been shown to interact specifically with metal centres like Hg II and Tl III . [18][19][20] In the present work the reactions of the deoxyribonucleoside and ribonucleotide of 4-thiouridine have been studied.…”

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confidence: 99%

Kinetics of interaction of the thione unit in 4-thio-2′-deoxyuridine and 4-thiouridine 5′-monophosphate with rapidly reacting gold(III) complexes ‡

Ericson¹,

Arthur²,

Coleman³

et al. 1998

J. Chem. Soc., Dalton Trans.

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The Isolation of 4-Thiouridylic Acid from the Soluble Ribonucleic Acid of Escherichia coli

Cited by 234 publications

References 16 publications

Sulfur-deficient transfer ribonucleic acid. Natural substrate for ribonucleic acid sulfurtransferase from Escherichia coli

Sulfur-deficient transfer ribonucleic acid. Natural substrate for ribonucleic acid sulfurtransferase from Escherichia coli

Photoelectron spectra of 2-thiouracil, 4-thiouracil, and 2,4-dithiouracil

Kinetics of interaction of the thione unit in 4-thio-2′-deoxyuridine and 4-thiouridine 5′-monophosphate with rapidly reacting gold(III) complexes ‡

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