The isolation, identification , physiological and biochemical properties of salt-tolerant strains

Abstract

： [Objective] By screening salt-tolerant strains , provide support for salted wastewater.[Method]With high salted water high efficiency salt activated sludge acclimation salt high efficiency cultivation as the source of strain , isolated and purified salt-tolerant strains , by measuring the salt-tolerant strains to biological growth OD 600 of different salinity , 3 dominant strains SP1、SP3、SP7 were selected for salt-tolerance.Combining the basic shape , physiological and biochemical characteristics and 16S rRNA sequence analysis , identification of these 3 are salt tolerant strains Zymomonas(Halomonas sp.) .[Result] The growth of salt-tolerant strains were the best when the temperature was 30 , the pH value was 7-8 and the salt concentration was 15% ， under this condition, the removal rate of COD and ammonia nitrogen reached 75% and 62% respectively.When the salinity reached 18%, the salt tolerance declined and the growth of salttolerant strains were not adapted to the salt tolerance.