The Isolation and the Biofilm Formation of Uropathogens in the Patients with Catheter Associated Urinary Tract Infections (UTIs)

Abstract

Background: Urinary tract infections are the most commonly acquired bacterial infections and they account for an estimated 25-40% of the nosocomial infections. The microbial biofilms pose a public health problem for the persons who require indwelling medical devices, as the microorganisms in the biofilms are difficult to treat with antimicrobial agents. Aims:The present study included the isolation and the biofilm formation of the uropathogens in patients with catheter associated urinary tract infections. Methods and Materials:This prospective analysis which was carried out over a period of two months, included 50 urine samples from catheterized patients with symptoms of UTI. Following their isolation and identification, all the isolates were subjected to the biofilm detection by the tube adherence method and the Congo Red agar method.Results: E.coli was found to be the most frequently isolated uropathogen 35(70%), followed by Klebsiella pneumoniae 8(16%), Pseudomona aeruginosa 2(4%), Acinetobacter spp 1(2%), coagulase negative Staphylococci 3(6%) and Enterococci spp 1(2%). In the current study, 30 (60%) strains were positive in vitro for the biofilm production. Conclusion:To conclude, there was significant bacteriuria in all the symptomatic catheterized patients and E.coli was the most frequent isolate. Diabetes (44%) was the most common factor which was associated with the UTIs in the catheterized patients.

Biofilm Producing Uropathogens and Drug Resistance: Dual Foe for Patients on Urinary Catheter

“…This finding correlated with few other previous studies (Hassin 1991;Niveditha et al, 2012;Sayal et al, 2014). The presence and expression of various virulence factors like, adhesions (e.g., type 1 and P. fimbriae) and toxins (e.g., haemolysin) are responsible for uropathogenic E. coli to be the most common cause of UTI (Bien et al, 2012;Reisner et al, 2014).…”

A Study on the Detection of Biofilm Formation by Multidrug Resistant Uropathogenic Isolates in a Tertiary Care Hospital

“…It correlates with the study done by Ramanath Katta Venkatesh et al, [12] which tells that 43% of diabetic patients have UTI. It also correlates with the study of Hamdan Z Hamdan et al [13] and S Niveditha et al [1] In our study, the polymicrobial pattern of growth was found to be 8%. It was around 2.9% in the study done by J. Janifer et al [14] In this study Gram negative bacilli (73.15%) were more common than Gram positive cocci (26.25%), which correlates with the finding of Devanand Prakash et al [11] that out of 155 bacterial uropathogens, 140 (90.32%) were Gram negative and 15 (9.68%) were Gram positive isolates.…”

Infection surveillance analysis of catheter associated urinary tract infections in obstetrics and gynecology department of a tertiary care hospital of Central India

“…Similarly, study done by S Nivedita found that catheter associated bacteuria increases when duration of catheterization increases. 11 Commonly isolated microbes in present study are E. Coli and Enterococcus respectively like Hootan TM et al 12 To reduce the laboratory workload considerably and for screening purposes pre and post catheterization urine samples are to be checked by bedside dipstick methods of high negative predictive value. 13 In order to restraint the enigma, periodic training sessions for all health care workers based on bundled care interventions supervisory checklists are needed.…”