The isolation and improvement of industrially important microorganisms

Stanbury, Peter F.; Whitaker, Allan; Hall, Stephen J.

doi:10.1016/b978-0-08-099953-1.00003-x

Cited by 6 publications

(7 citation statements)

References 201 publications

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“…To ensure the reproducibility of the experimental conditions, we characterized the morphology of the strain both in the inoculum and in the different fermentation steps. We considered, as well, the risk of introducing mutations and strain degeneration after each transfer and cell division by the liquid-liquid sub-culturing required to obtain enough inoculum for the study [16]. This effect has been previously observed in studies comparing jasmonic acid production by a fresh and a sub-cultured producing strain, which concluded that liquid-liquid sub-culturing led to a significative downfall of jasmonic acid production by Lasiodiplodia theobromae [17].…”

Section: Standardization Of Cultivation Conditions Of Dothiora Spmentioning

confidence: 99%

Multicomponent Analysis of the Differential Induction of Secondary Metabolite Profiles in Fungal Endophytes

et al. 2016

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Small molecule histone deacetylase (HDAC) and DNA methyltransferase (DNMT) inhibitors are commonly used to perturb the production of fungal metabolites leading to the induction of the expression of silent biosynthetic pathways. Several reports have described the variable effects observed in natural product profiles in fungi treated with HDAC and DNMT inhibitors, such as enhanced chemical diversity and/or the induction of new molecules previously unknown to be produced by the strain. Fungal endophytes are known to produce a wide variety of secondary metabolites (SMs) involved in their adaptation and survival within higher plants. The plant-microbe interaction may influence the expression of some biosynthetic pathways, otherwise cryptic in these fungi when grown in vitro. The aim of this study was to setup a systematic approach to evaluate and identify the possible effects of HDAC and DNMT inhibitors on the metabolic profiles of wild type fungal endophytes, including the chemical identification and characterization of the most significant SMs induced by these epigenetic modifiers.

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Section: Standardization Of Cultivation Conditions Of Dothiora Spmentioning

confidence: 99%

Multicomponent Analysis of the Differential Induction of Secondary Metabolite Profiles in Fungal Endophytes

et al. 2016

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“…For example, Mahara et al (2021) found that three folate-producing LAB ( Lactobacillus plantarum 4C261, Lactobacillus fermentum JK13, and Lactobacillus rhamnosus R23) become folate consumers when grown in folate-containing media, indicating that their folate-biosynthetic pathways are tightly regulated by feedback inhibition. Genetically engineered strains must therefore be designed to modify the regulatory mechanism of folate biosynthesis to allow the development of folate overproducing strains ( Stanbury et al, 2017 ). In addition, selection of analog-resistant mutants can be a natural strategy for obtaining folate-overproducing mutants ( Wegkamp, 2008 ; Okoroafor et al, 2019 ; Zhang et al, 2020 ).…”

Section: Folate-overproducing Bacteriamentioning

confidence: 99%

“…The stress-resistance method is a classic mutagenesis technique that can trigger spontaneous and directed mutagenesis to obtain mutants with desired phenotypic characteristics (Wegkamp, 2008;Renault, 2010). In this process, microorganisms are exposed to compounds that can inhibit their biosynthetic pathways, such as chemical analogs of targeted metabolites or intermediates (Wegkamp, 2008;Stanbury et al, 2017). Owing to their highly similar structures, these metabolic analogs can compete for binding to biosynthetic enzymes by imitating their control properties, interfering with metabolite biosynthesis, and inhibiting growth (Stanbury et al, 2017).…”

Section: Folate Overproduction By Chemical Analog Stress Resistancementioning

confidence: 99%

Hypothetical Regulation of Folate Biosynthesis and Strategies for Folate Overproduction in Lactic Acid Bacteria

Mahara,

Nuraida,

Lioe

et al. 2023

Prev. Nutr. Food Sci.

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Folate (vitamin B9) is an essential nutrient for cell metabolism, especially in pregnant women; however, folate deficiency is a major global health issue. To address this issue, folate-rich fermented foods have been used as alternative sources of natural folate. Lactic acid bacteria (LAB), which are commonly involved in food fermentation, can synthesize and excrete folate into the medium, thereby increasing folate levels. However, screening for folate-producing LAB strains is necessary because this ability is highly dependent on the bacterial strain. Some strains of LAB consume folate, and their presence in a fermentation mix can lower the folate levels of the final product. Since microorganisms efficiently regulate folate biosynthesis to meet their growth needs, some strains of folate-producing LAB can deplete folate levels if folate is available in the media. Such folate-efficient producers possess a feedback inhibition mechanism that downregulates folate biosynthesis. Therefore, the application of folate-overproducing strains may be a key strategy for increasing folate levels in media with or without available folate. Many studies have been conducted to screen folate-producing bacteria, but very few have focused on the identification of overproducers. This is probably because of the limited understanding of the regulation of folate biosynthesis in LAB. In this review, we discuss the roles of folate-biosynthetic genes and their contributions to the ability of LAB to synthesize and regulate folate. In addition, we present various hypotheses regarding the regulation of the feedback inhibition mechanism of folate-biosynthetic enzymes and discuss strategies for obtaining folate-overproducing LAB strains.

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“…Isolation of microorganisms was performed according to Stanbury et al (1995). One gram of each soil sample was suspended in 10 mL of sterilized water, vortexed for 1 min and then sedimented.…”

Section: Isolation Of Bacterial and Fungal Isolatesmentioning

confidence: 99%

“…Some microbial species were isolated based on the selection of the desired character (Stanbury et al, 1995). The growth of the bacterial and fungal isolates on cellobiose agar or defatted soybean flour agar showed that they were able to utilize cellobiose and soybean flour.…”

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confidence: 99%

Assessment of Genistein and Daidzein Production By Some Local Fungal and Bacterial Isolates

El-Shazly

El-Deen

Ibrahim

et al. 2017

Egypt. J. Microbiol.

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T WENTY-THREE different bacterial and fungal isolates were grown and screened for their capability to transform soy glycosides to their aglycone forms with higher titer of antioxidant activity compared to unfermented soy flour. Most of the bacterial isolates showed higher amounts of daidzein than of genistein, which are the aglycone products of daidzin and genistin. After fermentation of soybean flour using bacterial and fungal isolates, the content of isoflavone aglycones varied from 0.0 to 431.89 µg/g compared to unfermented autoclaved soybean flour. Extracellular β-glucosidase activity was ranged from 1.22 to 11.56 mU/mL and 0.3-534.3 U/mL for bacterial and fungal isolates, respectively, while, bacterial cell-bound β-glucosidase ranged from 44.72 to 128.89 mU/mL. Most of the bacterial isolates more efficiently transformed daidzin and genistin into the aglycones than fungal isolates. Among the tested bacterial isolates, the most potent one was selected, characterized according to the morphological and 16S rDNA sequence analysis and identified as Bacillus licheniformis NRC24.

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The isolation and improvement of industrially important microorganisms

Cited by 6 publications

References 201 publications

Multicomponent Analysis of the Differential Induction of Secondary Metabolite Profiles in Fungal Endophytes

Multicomponent Analysis of the Differential Induction of Secondary Metabolite Profiles in Fungal Endophytes

Hypothetical Regulation of Folate Biosynthesis and Strategies for Folate Overproduction in Lactic Acid Bacteria

Assessment of Genistein and Daidzein Production By Some Local Fungal and Bacterial Isolates

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