The isolation and growth of axenic cultures of planktonic blue—green algae

Fitzsimons, A. G.

doi:10.1080/00071618400650161

Cited by 14 publications

(6 citation statements)

References 13 publications

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“…nutrient solution, light, temperature, semi-continuous dilution mode) are low compared with other planktonic algae but they are similar with results from other studies under similar conditions (Foy et al, 1976;Fitzsimons and Smith, 1984;De Nobel et al, 1998).…”

Section: Discussionsupporting

confidence: 93%

Response of cylindrospermopsin production and release in Aphanizomenon flos-aquae (Cyanobacteria) to varying light and temperature conditions

et al. 2009

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Section: Discussionsupporting

confidence: 93%

Response of cylindrospermopsin production and release in Aphanizomenon flos-aquae (Cyanobacteria) to varying light and temperature conditions

et al. 2009

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“…Similar observations have been reported where several microalgal species grew better in bacteria-contaminated cultures than in axenic ones (e.g. [2]).…”

Section: Introductionsupporting

confidence: 89%

Algal growth enhancement by bacteria: Is consumption of photosynthetic oxygen involved?

Mouget

Dakhama

Lavoie

et al. 2006

FEMS Microbiology Ecology

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Pseudomonas diminuta and P. vesicularis, two obligate aerobes isolated from laboratory algal cultures, stimulated the growth of the green microalgae Scenedesmus bicellularis and Chlorella sp., without releasing any growth promoting substance. An intimate contact between both microorganisms was necessary for significant algal growth enhancement. The possibility of algal growth stimulation by bacterial attenuation of photosynthetic oxygen tension was indirectly examined by simulating the effect of bacteria through a physical removal of oxygen (air suction). Vacuum‐treated cultures showed an increase in growth rate and photosynthetic activity as compared to the control, a result which cannot be explained by differences in CO2/HCO3− pump activity. In the presence of P. diminuta, the photosynthetic activity of S. bicellularis was more strongly stimulated under a limited concentration of inorganic carbon. It is suggested that, apart from a CO2 supply, aerobic bacteria can promote algal growth by reducing the photosynthetic oxygen tension within the microenvironment of the algal cells, thereby creating more favorable conditions for optimal photosynthetic algal growth.

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“…Nonaxenic cultures. The purification of cyanobacteria can be a difficult and time-consuming procedure, and often they are cultivated more easily when accompanied by heterotrophic bacteria (9,13). Therefore, some strains growing as unicyanobacterial but nonaxenic cultures have been included in the bacteriological classification system (9).…”

mentioning

confidence: 99%

PCR primers to amplify 16S rRNA genes from cyanobacteria

Nübel

García-Pichel

Muyzer

1997

Appl Environ Microbiol

1,227

590

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We developed and tested a set of oligonucleotide primers for the specific amplification of 16S rRNA gene segments from cyanobacteria and plastids by PCR. PCR products were recovered from all cultures of cyanobacteria and diatoms that were checked but not from other bacteria and archaea. Gene segments selectively retrieved from cyanobacteria and diatoms in unialgal but nonaxenic cultures and from cyanobionts in lichens could be directly sequenced. In the context of growing sequence databases, this procedure allows rapid and phylogenetically meaningful identification without pure cultures or molecular cloning. We demonstrate the use of this specific PCR in combination with denaturing gradient gel electrophoresis to probe the diversity of oxygenic phototrophic microorganisms in cultures, lichens, and complex microbial communities.

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The isolation and growth of axenic cultures of planktonic blue—green algae

Cited by 14 publications

References 13 publications

Response of cylindrospermopsin production and release in Aphanizomenon flos-aquae (Cyanobacteria) to varying light and temperature conditions

Response of cylindrospermopsin production and release in Aphanizomenon flos-aquae (Cyanobacteria) to varying light and temperature conditions

Algal growth enhancement by bacteria: Is consumption of photosynthetic oxygen involved?

PCR primers to amplify 16S rRNA genes from cyanobacteria

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