The isolation and characterization of Type 1 metallothionein (MT) cDNA from a heavy-metal-tolerant plant, Festuca rubra cv. Merlin

Ma, Ming; Lau, Arthur P.S.; Jia, Yantao; Tsang, Wing-Keung; Lam, S.K.S.; Tam, N.F.Y.; Wong, Yau Shu

doi:10.1016/s0168-9452(02)00334-5

Cited by 60 publications

(33 citation statements)

References 34 publications

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“…Our data indicate that EhMT1 is induced by treatments with Cu, H 2 O 2 , and heat shock. The expression of plant type 1 MT genes is induced by Cu in a number of plant species (Zhou and Goldsbrough 1994;Hsieh et al 1995;Snowden et al 1995;Choi et al 1996;García-Hernández et al 1998;Morris et al 1999;Giordani et al 2002;Ma et al 2003;Guo et al 2003;Roosens et al 2004). In this study, Cu treatment increased EhMT1 expression more strongly in roots than in leaves.…”

Section: Discussionmentioning

confidence: 50%

“…AtMT1 from Arabidopsis enhances Cu resistance in yeast cells (Zhou and Goldsbrough 1994). Functional complementation studies have shown that mcMT1 from the metal-tolerant grass Festuca rubra functions to sequester Cu and other metal ions (Ma et al 2003).…”

Section: Discussionmentioning

confidence: 99%

“…In some plant species, Cu tolerance is associated with constitutively enhanced transcript levels of a type 2 MT (Murphy and Taiz 1995;van Hoof et al 2001;Mengoni et al 2003). Gene expression analyses and functional testing in yeast have revealed a role for MTs in Cu homeostasis and detoxification (Zhou and Goldsbrough 1994;Ma et al 2003;Roosens et al 2004;Hassinen et al 2007). In addition, plant MTs are involved in important physiological processes, including fruit ripening (Moyle et al 2005), root development, embryo germination , and suberization (Mir et al 2004).…”

Section: Introductionmentioning

confidence: 99%

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Cloning and characterization of a type 1 metallothionein gene from the copper-tolerant plant Elsholtzia haichowensis

Xia

Yuan

et al. 2012

Acta Physiol Plant

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EhMT1, a type 1 metallothionein (MT) gene, was cloned from Elsholtzia haichowensis, a Cu-tolerant plant. Typical of plant type 1 MTs, EhMT1 encodes a putative peptide of 74 amino acid residues containing cysteine-rich domains. Sequence comparisons with various databases revealed strong similarities at the nucleotide and amino acid levels between EhMT1 and the type 1 MT of Mimulus guttatus. EhMT1 transcription was greater in the roots than in the leaves, and was markedly increased by treatments with Cu, H 2 O 2 , and heat shock. EhMT1-GUS was localized to the cytoplasm of onion epidermal cells. Escherichia coli cells expressing pET-30a-EhMT1 were more tolerant to Cu and accumulated more Cu than control cells. Our results show that EhMT1 is involved in Cu tolerance and accumulation in E. haichowensis.

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Section: Discussionmentioning

confidence: 50%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cloning and characterization of a type 1 metallothionein gene from the copper-tolerant plant Elsholtzia haichowensis

Xia

Yuan

et al. 2012

Acta Physiol Plant

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“…Graminaceous plants, like other so-called metal-tolerant plants, mostly sequester metals in roots to protect reproductive and photosynthetic tissues (Li et al, 2000;Simon, 2005). The ability to store metals in underground tissues is used in phytoremediation to reinstall a vegetation cover on heavily contaminated areas and limit the propagation of metals into the food chain (Smith and Bradshaw, 1992;Cunningham et al, 1995;Vangronsveld et al, 1995;Ma et al, 2003;Mench et al, 2003;Krämer, 2005). Panfili et al (2005) showed that the grass species Festuca rubra (red fescue) and Agrostis tenuis (colonial bentgrass) accelerate the weathering of zinc sulfide when grown on contaminated dredged sediment, thus increasing Zn bioavailability in the rhizosphere.…”

Section: Formation Of Zn-ca Phyllomanganate Nanoparticles In Grass Rootsmentioning

confidence: 99%

Formation of Zn–Ca phyllomanganate nanoparticles in grass roots

Lanson

Marcus

Fakra

et al. 2008

Geochimica et Cosmochimica Acta

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International audienceIt is now well established that a number of terrestrial and aquatic microorganisms have the capacity to oxidize and precipitate Mn as phyllomanganate. However, this biomineralization has never been shown to occur in plant tissues, nor has the structure of a natural Mn(IV) biooxide been characterized in detail. We show that the graminaceous plant Festuca rubra (red fescue) produces a Zn-rich phyllomanganate with constant Zn:Mn and Ca:Mn atomic ratios (0.46 and 0.38, respectively) when grown on a contaminated sediment. This new phase is so far the Zn-richest manganate known to form in nature (chalcophanite has a Zn:Mn ratio of 0.33) and has no synthetic equivalent. Visual examination of root fragments under a microscope shows black precipitates about ten to several tens of microns in size, and their imaging with backscattered and secondary electrons demonstrates that they are located in the root epidermis. In situ measurements by Mn and Zn K-edge extended X-ray absorption fine structure (EXAFS) spectroscopy and X-ray diffraction (XRD) with a micro-focused beam can be quantitatively described by a single-phase model consisting of Mn(IV) octahedral layers with 22% vacant sites capped with tetrahedral and octahedral Zn in proportions of 3:1. The layer charge deficit is also partly balanced by interlayer Mn and Ca. Diffracting crystallites have a domain radius of 33 Å in the ab plane and contain only 1.2 layers (not, vert, similar8.6 Å) on average. Since this biogenic Mn oxide consists mostly of isolated layers, basal 00l reflections are essentially absent despite its lamellar structure. Individual Mn layers are probably held together in the Mn–Zn precipitates by stabilizing organic molecules. Zinc biomineralization by plants likely is a defense mechanism against toxicity induced by excess concentrations of this metal in the rhizosphere

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“…For Arabidopis, gene silencing experiments revealed that MT1 was required to protect this species against the toxic effects of Cd, and that it may be specifically related to the reduction and control of toxic levels of such metal in cells (Zimeri et al, 2005). In addition, one MT1, when isolated from a cDNA library induced by Cu in Festuca rubra, showed tolerance to Cd in studies of functional complementation using vectors with cDNA MT1 (Ma et al, 2003). Furthermore, transformation of Nicotiana tabacum with an MT gene, isolated from Silene vulgaris, increased tolerance to and accumulation of Cd in such species (Gorinova et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Preliminary analysis of expressed sequences of genes in Genipa americana L. plant roots exposed to cadmium in nutrient solution

Souza

Almeida²,

Hora³

et al. 2008

Genet. Mol. Res.

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ABSTRACT. Many cell functions are redundantly executed in cells, and the experimental approaches that analyze the group of proteins, whose expression is modified in a specific functional condition, enable the identification of the group of proteins that are expressed under stress conditions. The objective of the present study was the evaluation of the genetic expression induced by cadmium (Cd) in Genipa americana L. (Rubiaceae) plants cultivated in nutritive solution, in order to help further studies concerning its use as a plant phytoremediator of such a metallic element. Plants were exposed to increasing concentrations of Cd (0.5, 1, 2, 4, 8, and 16 mg/L), together with the control, in nutritive solution. After the application of the treatments, root tips were harvested for the construction of a cDNA library. Of the 165 expressed sequence tags (ESTs) generated with the construction of the cDNA library, 81 showed homology to genes deposited in the NCBI database, 67 did not show similarity to any available gene, and 17 ESTs ESTs of genes in G. americana exposed to cadmium demonstrated homology with unknown genes. Of the most abundant cDNAs, 16 ESTs were similar to sequences of metallothionein genes. The analysis of ESTs, obtained from the root of G. americana through the construction of a cDNA library, allowed the identification of genes probably associated with proteins and enzymes related to the defense mechanisms of plants when they undergo biotic and abiotic stresses.

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The isolation and characterization of Type 1 metallothionein (MT) cDNA from a heavy-metal-tolerant plant, Festuca rubra cv. Merlin

Cited by 60 publications

References 34 publications

Cloning and characterization of a type 1 metallothionein gene from the copper-tolerant plant Elsholtzia haichowensis

Cloning and characterization of a type 1 metallothionein gene from the copper-tolerant plant Elsholtzia haichowensis

Formation of Zn–Ca phyllomanganate nanoparticles in grass roots

Preliminary analysis of expressed sequences of genes in Genipa americana L. plant roots exposed to cadmium in nutrient solution

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