The isolation and characterization of glycopeptides and mucopolysaccharides from plasma membranes of normal and regenerating livers of rats

Akasaki, Moriaki; Kawasaki, Toshisuke; Yamashina, Ikuo

doi:10.1016/0014-5793(75)80350-4

Cited by 52 publications

(16 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increase in sialylation of the membrane glycoproteins upon transformation has mainly been attributed to increased branching of N ‐linked glycoproteins; 8 however, gp120 in ZAH appears to be an O ‐linked oligosaccharide as it does not bind to LCA or PHA‐P, although ZAH C membranes contain at least one specific fucose‐containing N ‐linked complex‐type sialo‐glycoprotein also. The presence of sialylated O ‐linked and N ‐linked glycoproteins, as observed in our system, has also been reported in a number of rat ascites HCC 27,30 and in Novikoff HCC 31 . At least in one system, namely the AH 66 HCC, 32 the sialic acid content was more in the O ‐linked glycopeptides than in the N ‐linked glycopeptides, as in the case of ZAH.…”

Section: Discussionsupporting

confidence: 87%

Significance of glycosylation patterns of rat Zajdela ascitic hepatocellular carcinoma: Effect of enzymatic removal of surface sialic acid on humoral response

Sudhakar

Mangamoori

2008

J of Gastro and Hepatol

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 87%

Significance of glycosylation patterns of rat Zajdela ascitic hepatocellular carcinoma: Effect of enzymatic removal of surface sialic acid on humoral response

Sudhakar

Mangamoori

2008

J of Gastro and Hepatol

View full text Add to dashboard Cite

show abstract

“…Hardly any contamination of the latter tissue can be expected in our experiment using late embryonic liver as a control for hepatomas. Furthermore, regenerating rat liver has also been used as a control for rat hepatoma to the same purpose and with the same result (Akasaki, Kawasaki and Yamashina, 1975;Smets et al, 1975). Thus neither proliferation per se (c.f.…”

Section: Methodsmentioning

confidence: 87%

Comparison of cell-surface glycoproteins of rat hepatomas and embryonic rat liver

Beek¹,

Emmelot²,

Homburg³

1977

Br J Cancer

View full text Add to dashboard Cite

Summary.-Cell-surface glycoprotein of 3 rat hepatoma strains and late-embryonic liver was metabolically labelled in vivo with [3H]-or [14C]-fucose. Trypsinization of the cells and exhaustive pronase digestion of combined hepatoma-liver trypsinates followed by gel filtration over Sephadex-Biogel mixtures, yielded elution profiles that contained more early-eluting (high-mol.-wt.) glycopeptides for hepatomas than for liver. At least 3 factors were identified which acted to augment the fraction of earlyeluting tumour glycopeptides: (a) increase of neuraminidase-sensitive sialic acid, (b) increase of neuraminidase -insensitive sialic acid that was sensitive to mild HCI hydrolysis, and (c) presence of sugar sulphate groups contributing to a restricted extent, relative to possible unknown factor(s). Whether (a), (b) or (c) operated depended on the hepatoma strain or its mode of growth. Notwithstanding these differences in the nature of the increase in early-eluting glycopeptides, the increase itself appears not to be due to growth per se, nor to an embryonic expression, but rather may serve as a marker of tumourigenicity.

show abstract

“…5) might play a pivotal role in the multistep process of liver cell damage (36) or, in the Initiation of cell division following injury (37). Both assumptions arise from the previously discussed (28) and above briefly mentioned physiological functions of pericellular proteoheparan sulfate (6,(9)(10)(11)(12). In addition to the key role of changes of the plasmalemma in the pathogenesis of toxic liver injury (38)(39)(40)(41) structural alterations of the glycocalyx may have importance for Under the protective effects of diethyldithiOcarbamate (42) and of the natural flavonoid (*)cianidanol-3 (43-45) on the development of galactosainine-hepar titis, a less pronoünced Inhibition of proteogiycan synthesis was observed.…”

Section: Discussionmentioning

confidence: 99%

“…Heparan sulfate, the preponderant type of glycosaminoglycan in liver (7,8) occurs predominantly in the microenvironment of the cell, i.e. the external surface of the hepatocyte (6,(9)(10)(11)(12) and piay there control the exchange of metabolites, ions, fluids, and wateir, exert some effects on hepatocellular proliferation and may regulate the accessibility of cell surface receptors (13-15). The physiological significance of the galactosamine containing sulfated glycosaminoglycans (chondroitin sulfate, dermatan sulfate) in liver is not known.…”

Section: Introductionmentioning

confidence: 99%

The Sequence of Changes in the Biosynthesis of Sulfated Glycosaminoglycans in Acute, Experimental Liver Disease

Gressner¹,

Heinrigs²,

Grouls³

1982

CCLM

View full text Add to dashboard Cite

The incorporation of [ 35 S]sulfate into total and specific glycosaminoglycans of the liver of rats injured acutely by a singie intraperitoneal dose of either jD-galactosamine (700 and 350 mg/kg body weight, respectively) or thioacetamide (100 mg/kg body weight) was studied in vivo and in liver slices. Regardless of the chemical nature of the hepatotoxic agent and its mechanism of action the incorporation of isotope in vivo is changed biphasically: a rapidly occurring and strong Inhibition is followed by pronounced elevation in later stages of injury. In contrast to the dose-dependent Inhibition of heparan sulfate formation the production of chondroitin sulfate and dermatan sulfate is only slightly and for a rather short period of time diminished. The synthesis of the latter glycosaminoglycans is stimulated 3 to 4 fold 8 to 24 h after onset of liver damage, fhat of heparan sulfate about 2 fold. Impaired synthesis of heparan sulfate occurs before the elevation of the activities of aspartate aminotransferase, alanine aminotransferase, and leucine arylamidase in serum. The specific radioactivity of 3'-phosphoadenosine-5'-phosphosulfate remains nearly constant in galactosamine-damaged livers. Similar time-dependent changes of [ 3S S]sulfate incorporation into heparan sulfate, chondroitin sulfate and dermatan sulfate were measured in slices from livers injured in vivo for various times with -D-galactosamine or thioacetamide. Neither serum from normal nor galactosaniine-treated rats influences the total amount and the pattern of glycosaminoglycans synthesized in normal or injured liver slices. Hepatic glycosaminoglycan formation of 3.5 and 33 months old rats responds quantitatively and qualitatively in a simüar way to £>-galactosamine; 50% Inhibition is reached with about 80 mg/kg of the hepatotoxin. The Inhibition of heparan sulfate synthesis in early injured livers could not be reversed by addition of p-nitrophenyl-/3-Z)-xylopyranoside to the medium (final concentration l mmol/1), but in rats treated with diethyldithiocarbamate or the flavonoid (+)-cianidanol-3, prior to injection of Z)-galactosamine, a partial restoration of the diminished synthesis of total glycosaminoglycans and of heparan sulfate is achieved. Die Sequenz der Änderungen der Biosynthese sulfatierter Glykosaminoglykane bei akuten, experimentellen LebererkrankungenZusammenfassung: Der Einbau von [ 3 *S]Sulfat in die gesamten und spezifischen Glykosaminoglykane der Leber von Ratten, die akut geschädigt wurden durch eine intraperitoneale Einzelinjektion von entweder Z)-Galaktosamin (700 mg/kg oder 350 mg/kg Körpergewicht) oder Thioacetamid (100 mg/kg), wurde in vivo und in Leberschnitten untersucht. Ungeachtet der chemischen Natur der hepatotoxischen Substanz und ihres Wirkungsmechanismus kommt es zu einer biphasischen Änderung des Isotopeinbaues: eine unmittelbar auftretende und starke Hemmung wird gefolgt von einer ausgeprägten Erhöhung in späteren Stadien der Schädigung. Im Gegensatz zu der dosisabhängigen Hemmung der Heparansulfatbildung ist die Pr...

show abstract

The isolation and characterization of glycopeptides and mucopolysaccharides from plasma membranes of normal and regenerating livers of rats

Cited by 52 publications

References 16 publications

Significance of glycosylation patterns of rat Zajdela ascitic hepatocellular carcinoma: Effect of enzymatic removal of surface sialic acid on humoral response

Significance of glycosylation patterns of rat Zajdela ascitic hepatocellular carcinoma: Effect of enzymatic removal of surface sialic acid on humoral response

Comparison of cell-surface glycoproteins of rat hepatomas and embryonic rat liver

The Sequence of Changes in the Biosynthesis of Sulfated Glycosaminoglycans in Acute, Experimental Liver Disease

Contact Info

Product

Resources

About