The Isolation and Characterization of Bacteriophages Infecting Obligately Thermophilic Strains of Bacillus

Sharp, Richard; Ahmad, Shafiq; Munster, Ann P.; Dowsett, B.; Atkinson, Tony

doi:10.1099/00221287-132-6-1709

Cited by 18 publications

(30 citation statements)

References 15 publications

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“…Henson et al (1987) Guest & Roberts (1983) Bullock et al (1987) Mallinder et al (1992) Liao etal. (1986) Moir et al (1979) Sharp et al (1986) Stratagene This study Moir etal. (1984) Guest & Roberts (1983) Woods & Guest (1987) Enzymology.…”

Section: Methodsmentioning

confidence: 94%

Molecular and Enzymological Evidence for Two Classes of Fumarase in Bacillus Stearothermophilus (var. Non-Diastaticus)

Reaney

Bungard²,

Guest³

1993

Journal of General Microbiology

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The gene vumABst) encoding an oxygen-labile fumarase of Bacillus stearothermophilus has been cloned and sequenced. The structural gene (1542 bp) encodes a product (FumA,J of M, 56788 containing 514 amino acid residues. The amino acid sequence is 23% identical (37% similar) to FumA and FumB, the labile [ 4 F d S ] -containing fumarases (Class I enzymes) of Escherichia coli. It exhibits no significant similarity to FumC and CitG, the stable fumarases (Class I1 enzymes) of E. coli and Bacillus subtilis (respectively). Enzymological studies indicated that FumA,,, resembles the iron-sulphur-containing fumarases in being dimeric (M, 2 x 58 500), oxygen labile and partially reactivated by Fe2+ plus DTT. ThefumA,, gene is the first gene encoding a Class I fumarase to be characterized in any organism other than E. coli. Enzymological and DNA-hybridization studies further indicated that B. stearothermophilus resembles E. coli in containing an oxygen-stable fumarase (Class I1 enzyme). Sequence comparisons revealed significant similarities between the Class I fumarases and the products of adjacent open-reading frames ( o r f l l and orfl2) located upstream of the macromolecular synthesis operon (rpsU-dnaGrpoD) at 67 min in the E.coli linkage map. Located downstream of fumA,,, there is an unidentified gene (orfl), which is homologous to the rhizobial nodB genes involved in the initiation of root nodule formation.

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Section: Methodsmentioning

confidence: 94%

Molecular and Enzymological Evidence for Two Classes of Fumarase in Bacillus Stearothermophilus (var. Non-Diastaticus)

Reaney

Bungard²,

Guest³

1993

Journal of General Microbiology

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“…However, utilizing the entire luxCDABE cassette provides all of the biochemical reactions necessary for bioluminescence without addition of exogenous substrate. Bacteriophage bioluminescent bioreporters contain a luxAB gene cassette inserted into their genome [8][9][10][11][12][13] . During an infection event the luxAB are expressed by the host bacterium, resulting in a bioluminescent phenotype.…”

Section: Bioluminescent Reportersmentioning

confidence: 99%

A model system for pathogen detection using a two-component bacteriophage/bioluminescent signal amplification assay

Bright

Carroll

Applegate

2004

Monitoring Food Safety, Agriculture, and Plant Health

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Microbial contamination has become a mounting concern the last decade due to an increased emphasis of minimally processed food products specifically produce, and the recognition of foodborne pathogens such as Campylobacter jejuni, Escherichia coli O157:H7, and Listeria monocytogenes. This research investigates a detection approach utilizing bacteriophage pathogen specificity coupled with a bacterial bioluminescent bioreporter utilizing the quorum sensing molecule from Vibrio fischeri, N-(3-oxohexanoyl)-homoserine lactone (3-oxo-C6-HSL). The 3-oxo-C6-HSL molecules diffuse out of the target cell after infection and induce bioluminescence from a population of 3-oxo-C6-HSL bioreporters (ROLux). E. coli phage M13, a well-characterized bacteriophage, offers a model system testing the use of bacteriophage for pathogen detection through cell-to-cell communication via a LuxR/3-oxo-C6-HSL system. Simulated temperate phage assays tested functionality of the ROLux reporter and production of 3-oxo-C6-HSL by various test strains. These assays showed detection limits of 10 2 cfu after 24 hours in a varietry of detection formats. Assays incorporating the bacteriophage M13-luxI with the ROLux reporter and a known population of target cells were subsequently developed and have shown consistent detection limits of 10 5 cfu target organisms. Measurable light response from high concentrations of target cells was almost immediate, suggesting an enrichment step to further improve detection limits and reduce assay time.

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“…In the absence of available hosts to infect, and as long as they are not damaged by external agents, phages can usually maintain their infective ability for decades (Guttman et al, 2005;Sharp et al, 1986).…”

Section: Antibiotic Resistancementioning

confidence: 99%

Phages as Therapeutic Tools to Control Major Foodborne Pathogens: Campylobacter and Salmonella

Carvalho¹,

Santos²,

Kropinski³

et al. 2012

Bacteriophages

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The Isolation and Characterization of Bacteriophages Infecting Obligately Thermophilic Strains of Bacillus

Cited by 18 publications

References 15 publications

Molecular and Enzymological Evidence for Two Classes of Fumarase in Bacillus Stearothermophilus (var. Non-Diastaticus)

Molecular and Enzymological Evidence for Two Classes of Fumarase in Bacillus Stearothermophilus (var. Non-Diastaticus)

A model system for pathogen detection using a two-component bacteriophage/bioluminescent signal amplification assay

Phages as Therapeutic Tools to Control Major Foodborne Pathogens: Campylobacter and Salmonella

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