The isolation and characterisation of a catalase-deficient mutant of barley (Hordeum vulgare L.)

Kendall, A. C.; Keys, A. J.; Turner, J. C.; Lea, Peter J.; Miflin, B. J.

doi:10.1007/bf00409139

Cited by 148 publications

(88 citation statements)

References 42 publications

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“…Decline of catalase activity in seedlings grown in 1% C02 (21% 02) and its reversal by transfer of seedlings to air. Seedlings were grown in air for 14 d then transferred to 1% CO2. Samples of leaves were harvested at the times indicated extracted, and assayed.…”

Section: Resultsmentioning

confidence: 99%

“…The observation that catalase-deficient mutants of barley are chlorotic in air but grow normally in high CO2 (14) firmly established that the principal metabolic role of catalase in C3 leaves is the decomposition of peroxisomal H202 under photorespiratory conditions. Accordingly, the rapid loss of CAT-1 in 1% C02/2 1% 02 was initially presumed to be related to the suppression of photorespiration by high CO2.…”

Section: Regulation Of Catalase Activity In Tobacco By High C02mentioning

confidence: 94%

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Regulation of Catalase Activity in Leaves of Nicotiana sylvestris by High CO₂

Havir¹,

McHale²

1989

Plant Physiol.

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The effect of high CO2 (1% C02/21% 02) on the activity of specific forms of catalase (CAT-1, -2, and -3) (EA Havir, NA McHale [1987] Plant Physiol 84: 450-455) in seedling leaves of tobacco (Nicotiana sylvestris, Nicotiana tabacum) was examined. In high C02, total catalase activity decreased by 50% in the first 2 days, followed by a more gradual decline in the next 4 days. The loss of total activity resulted primarily from a decrease in CAT-1 catalase. In contrast, the activity of CAT-3 catalase, a form with enhanced peroxidatic activity, increased 3-fold in high CO2 relative to aiontrols after 4 days. Short-term exposure to high CO2 indicated that the 50% loss of total activity occurs in the first 12 hours. Catalase levels increased to normal within 12 hours after seedlings were retumed to air. When seedlings were transferred to air after prolonged exposure to high CO2 (13 days), the levels of CAT-1 catalase were partially restored while CAT-3 remained at its elevated level. Levels of superoxide dismutase activity and those of several peroxisomal enzymes were not affected by high CO2. Total catalase levels did not decline when seedlings were exposed to atmospheres of 0.04% C02/5% 02 or 0.04% C02/1% 02, indicating that regulation of catalase in high CO2 is not related directly to suppression of photorespiration. Antibodies prepared against CAT-1 catalase from N. tabacum reacted strongly against CAT-1 catalase from both N. sylvestris and N. tabacum but not against CAT-3 catalase from either species. This observation, along with the rapid changes in CAT-I and the much slower changes in CAT-3 suggest that one form is not directly derived from the other. concentration (5, 19) have been shown to influence catalase activity in mature plants. For example, Fair et al. (5) demonstrated that catalase activity was suppressed when barley was grown in 1 to 5% CO2 and in 2% 02. Other peroxisomal enzymes were suppressed also and thus the effect in barley may be generally on peroxisomal turnover. In cases where catalase regulation is independent of peroxisomal turnover (e.g. Refs. 4, 6, and this paper) the mechanism of regulation has not been determined but could be the result of changes in rates of synthesis, degradation, or by covalent modification of the protein. The determination by Eising et al. (3) that the sharp decline in catalase activity in greening sunflower cotyledons was due to the formation of an inactive but largely intact protein molecule may be pertinent to studies of environmentally related changes in catalase activity.We previously showed that catalase activity in leaves of tobacco seedlings was distributed among several forms, one ofwhich had enhanced peroxidatic activity and that there was a change in distribution with time of development (l1). Furthermore, transfer of the seedlings to 1% C02/2 1% 02 for 4 d reduced total catalase activity by about 50% and altered the distribution ofthe activities. Here we show that the decline in catalase in high CO2 is very rapid and results primarily from the los...

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Section: Resultsmentioning

confidence: 99%

Section: Regulation Of Catalase Activity In Tobacco By High C02mentioning

confidence: 94%

Regulation of Catalase Activity in Leaves of Nicotiana sylvestris by High CO₂

Havir¹,

McHale²

1989

Plant Physiol.

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“…Although many Arabidopsis mutants with defects in photorespiratory enzymes were isolated as conditionally lethal mutants requiring enriched CO, conditions for growth, this collection did not include any mutants with defects in catalase (Somerville, 1986). A similar screen allowed the isolation of a barley mutant with reduced catalase activity (Kendall et al, 1983). Although this barley mutant retains some catalase activity, H,O, accumulates under normal atmospheric (photorespiratory) conditions, resulting in lipid peroxidation and disruption of membrane function (Parker and Lea, 1983).…”

Section: Rlbsfcmentioning

confidence: 99%

Catalase Is Encoded by a Multigene Family in Arabidopsis thaliana (L.) Heynh

et al. 1996

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The catalase multigene family in Arabidopsis includes three genes encoding individual subunits that associate to form at least six isozymes that are readily resolved by nondenaturing gel electrophoresis. CATl and CAT3map to chromosome 1 , and CAT2maps to chromosome 4. The nucleotide sequences of the three coding regions are 70 to 72% identical. The amino acid sequences of the three catalase subunits are 75 to 84% identical and 87 to 94% similar, considering conservative substitutions. Both the individual isozymes and the individual subunit mRNAs show distinct patterns of spatial (organ-specific) expression. Six isozymes are detected in flowers and leaves and two are seen in roots. Similarly, mRNA abundance of the three genes varies among organs. All three mRNAs are highly expressed in bolts, and CAT2 and CAT3 are highly expressed in leaves.

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“…The success of the approach generated some of the earliest converts to the utility of Arabidopsis genetics. In addition, Peter Lea, Ben Miflin, Alf Keys, and colleagues at Rothamstead used similar approaches to isolate a rich collection of photorespiratory mutants of barley that have been extensively utilized in continuing studies of photorespiration (8,10).…”

mentioning

confidence: 99%