The isolated perfused rat heart: A model for studying myocardial hypoxia or ischaemia

Leiris, Joël de; Harding, D. P.; Pestre, S

doi:10.1007/bf01908032

Cited by 49 publications

(22 citation statements)

References 19 publications

(4 reference statements)

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“…The literature contains an increasing number of studies which use mouse hearts, which have smaller hearts, making intra-ventricular pressure recordings more difficult, having high heart rate, the miniaturization of equipment. 31 One of most commonly studied parameter in the Langendorff's method is the electrical activity of the heart. Electrical activity of perfused heart has been used in studies of cardiac arrhythmias, action potentials and T-wave alternans, ischemic preconditioning, and action potential propagation.…”

Section: Discussionmentioning

confidence: 99%

Langendorff's isolated perfused rat heart technique: a review

Lateef

Al-Masri

Alyahya

2015

Int J Basic Clin Pharmacol

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Section: Discussionmentioning

confidence: 99%

Langendorff's isolated perfused rat heart technique: a review

Lateef

Al-Masri

Alyahya

2015

Int J Basic Clin Pharmacol

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“…This contrasts with the perfused rat heart, for example, which has been extensively characterised and generally displays hlghly reproducible responses (De Leiris et al 1984;Galinanes & Hearse, 1990). …”

Section: Publication Of the Physiological Society 2221mentioning

confidence: 99%

Functional properties and responses to ischaemia‐reperfusion in Langendorff perfused mouse heart

Headrick¹,

Peart²,

Hack³

et al. 2001

Experimental Physiology

112

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Despite minimal model characterisation Langendorff perfused murine hearts are increasingly employed in cardiovascular research, and particularly in studies of myocardial ischaemia and reperfusion. Reported contractility remains poor and ischaemic recoveries variable. We characterised function in C57/BL6 mouse hearts using a ventricular balloon or apicobasal displacement and assessed responses to 10-30 min global ischaemia. We examined the functional effects of pacing, ventricular balloon design, perfusate filtration, [Ca"] and temperature. Contractility was high in isovolumically functioning mouse hearts (measured as the change in pressure with time (+dP/dt), 6000-7000 mmHg s-I) and was optimal at a heart rate of -420 beats min-', with the vasculature sub-maximally dilated, and the cellular energy state high. Post-ischaemic recovery (after 40 min reperfusion) was related to the ischaemic duration: developed pressure recovered by 82 f YO, 73 f 4u/, 68 f 3%, 57 f 2% and 41 f 5% after 10, 15, 20, 25 and 30 min ischaemia, respectively. Ventricular compliance and elastance were both reduced post-ischaemia. Post-ischaemic recoveries were lower in the apicobasal model (80 +-4 YO, 58 k 7 YO, 40 +-3 %, 32 +-7 % and 25 2 5 YO) despite greater reflow and lower metabolic rate (pre-ischaemic myocardial O2 consumption (Vo2,,,,) 127 f 15 vs. 198 k 17 p l O2 min-' g-'), contracture, enzyme and purine efflux. Electrical pacing slowed recovery in both models, small ventricular balloons (unpressurised volumes C 50-60 p1) artificially depressed ventricular function and recovery from ischaemia, and failure to filter the perfusion fluid to C 0.45 pm depressed pre-and post-ischaemic function. With attention to these various experimental factors, the buffer perfused isovolumically contracting mouse heart is shown to be stable and highly energised, and to possess a high level of contractility, The isovolumic model is more reliable in assessing ischaemic responses than the commonly employed apicobasal model. Experimental Physiology (200 1) 86.6, 703-7 16.

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“…Different phospholipids were scraped off the plate and quantifi ed using the organic phosphate assay. Glycogen content and glucose incorporation into glycogen were also measured ( 48,49 ). Briefl y, tissues were hydrolyzed at 100°C for 30 min in 40% KOH, followed by precipitation with 100% ethanol.…”

Section: Phosphatidate Phosphatase Enzymatic Assaymentioning

confidence: 99%

Relationship of glucose and oleate metabolism to cardiac function in lipin-1 deficient (fld) mice

Kok

Kienesberger

Dyck

et al. 2012

Journal of Lipid Research

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of cardiac ATP production ( 1 ). FAs are also incorporated into triacylglycerol (TG), which is stored in lipid droplets. Cardiac TG turnover is rapid ( 2 ) and FAs released from TG can contribute up to 10-20% of ATP production ( 3 ). Plasma FA and TG levels rise in insulin resistance and diabetes ( 4, 5 ), and cardiac FA utilization can increase to supply as much as 90% of ATP ( 6, 7 ). Despite this increase in FAO, FA uptake can exceed the ability of the heart to utilize FAs, leading to excess accumulation of TG in cardiomyocytes ( 6-9 ). The compensatory changes that occur in response to excessive FA availability eventually become maladaptive, and the heart is locked in a pathological and infl exible metabolic state that contributes to cardiac dysfunction ( 6, 7, 10 ). As such, maintenance of metabolic fl exibility is essential for normal cardiac function. Whereas many proteins have been identifi ed to contribute to cardiac FA uptake and metabolism ( 1, 10 ), relatively little information is available about the role of lipin-1 in the heart. Lipin-1 is an important protein involved in regulating both TG synthesis and FAO in other organs ( 11 ).Instead of being considered as mutually antagonistic processes, FAO and TG synthesis are now widely viewed as being companion pathways because augmenting cardiac TG synthesis increases FAO ( 12 ). Moreover, FAs released through lipolysis of endogenous TG stores contribute signifi cantly to FAO ( 3,13 ). Lipin-1 could play a prominent role in this regulation as it is a bifunctional protein involved in regulating both TG synthesis and FAO in the The constant requirement for ATP in the beating heart is satisfi ed by the utilization of diverse substrates, such as fatty acids (FA), glucose, lactate, ketones, and amino acids, with FA oxidation (FAO) providing the majority (50-75%)

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The isolated perfused rat heart: A model for studying myocardial hypoxia or ischaemia

Cited by 49 publications

References 19 publications

Langendorff's isolated perfused rat heart technique: a review

Langendorff's isolated perfused rat heart technique: a review

Functional properties and responses to ischaemia‐reperfusion in Langendorff perfused mouse heart

Relationship of glucose and oleate metabolism to cardiac function in lipin-1 deficient (fld) mice

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