The irradiation of hepatocytes with He‐Ne laser causes an increase of cytosolic free calcium concentration and an increase of cell membrane potential, correlated with it, both increases taking place in an oscillatory manner

Vacca, Rosa Anna; Moro, Loredana; Petragallo, Vito A.; Greco, Margherita; Fontana, Fabrizio; Passarella, Salvatore

doi:10.1080/15216549700204821

Cited by 13 publications

(18 citation statements)

References 8 publications

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“…Two circumstances considering complex changes in the mitochondrial characteristics of retrograde signaling should be underlined. First, there exists a unique mitochondrial oscillator that depends on oxidative phosphorylation, ROS and permeability of mitochondrial inner membrane ion channels (61,62). It was hypothesized that the balance between O 2 •− efflux through inner membrane anion channels and the intracellular ROS scavenging capacity plays a key role in the oscillation mechanism.…”

Section: Ros Signalingmentioning

confidence: 99%

Mitochondrial Signaling in Mammalian Cells Activated by Red and Near‐IR Radiation

Karu

2008

Photochem & Photobiology

477

299

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Mitochondrial signaling is an information channel between the mitochondrial respiratory chain and the nucleus for the transduction signals regarding the functional state of the mitochondria. The present review examines the question whether radiation of visible and near-IR (IR-A) radiation can activate this retrograde-type cellular signaling pathway. Experimental data about modulation of elements of mitochondrial retrograde signaling by the irradiation (mitochondrial membrane potential DY m , reactive oxygen species ROS, Ca 2+ , NO • , pH i , fissionfusion homeostasis of mitochondria) are reviewed. The terminal enzyme of the mitochondrial respiratory chain cytochrome c oxidase is considered as the photoacceptor. Functions of cytochrome c oxidase as a signal generator as well as a signal transducer in irradiated cells are outlined.

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Section: Ros Signalingmentioning

confidence: 99%

Mitochondrial Signaling in Mammalian Cells Activated by Red and Near‐IR Radiation

Karu

2008

Photochem & Photobiology

477

299

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“…Laser irradiation has been shown to induce changes in intracellular Ca 2+ concentrations in macrophages, hepatocytes [17], and osteoblasts [18] in vitro. In this study, we first demonstrated an immediate increase in intracellular calcium levels 15 and 30 min after 1 J/ cm 2 laser stimulation in tenocytes.…”

Section: Discussionmentioning

confidence: 99%

Second messengers mediating the proliferation and collagen synthesis of tenocytes induced by low-level laser irradiation

Chen¹,

Huang²,

Sun

et al. 2014

Lasers Med Sci

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For decades, low-level laser therapy (LLLT) has widespread applications in tendon-related injuries. Although the therapeutic effect of LLLT could be explained by photostimulation of target tissue and cells, how tenocytes sense photonic energy and convert them into cascades of cellular and molecular events is still not well understood. This study was designed to elucidate the effects of LLLT on cell proliferation and collagen synthesis by examining the associated second messengers including ATP, Ca(2+), and nitric oxide using rat Achilles tenocytes. Moreover, proliferating cell nuclear antigen (PCNA) and transforming growth factor-β1 (TGF-β1) related to cell proliferation and matrix metabolism were also studied. The results showed that 904 nm GaAs laser of 1 J/cm(2) could significantly increase the MTT activity and collagen synthesis of tenocytes. Second messengers including ATP and intracellular Ca2+ were increased after laser treatment. Quantitative PCR analysis of tenocytes treated with laser revealed up-regulated expression of PCNA, type I collagen, and TGF-β1. Besides, laser-induced TGF-β1 expression was significantly inhibited by extracellular signal-regulated kinase (ERK) specific inhibitor (PD98059). The findings suggested that LLLT stimulated ATP production and increased intracellular calcium concentration. Directly or indirectly via production of TGF-β1, these second messengers mediated the proliferation of tenocytes and synthesis of collagen.

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“…correspondientes a hepatocitos de rata estimuladas con dosis crecientes de láser infrarrojo. En este contexto, nuestros resultados son disímiles en cuanto a la energía utilizada puesto que Greco et al (1996Greco et al ( , 2001) y Vacca et al (1997) utilizan 0.24 J/ cm 2 como energía activadora sobre hepatocitos probando grandes modificaciones de la función celular producto de dicha estimulación.…”

Section: Discussionunclassified

“…Así, Vacca et al (1993) comprueban activación en la replicación de DNA mitocondrial; Cieslar et al (1995) determinaron un notable incremento en las actividades enzimá-ticas tanto de membranas plasmáticas como mitocondriales. Vacca et al (1997) comunicaron que la irradiaciones infrarrojas generan aumentos considerables del calcio citoplasmático libre en relación con el potencial de membrana plasmática; del mismo modo, Greco et al (2001) precisaron que la estimulación con esta energía produce incremento en la concentración de calcio mitocondrial generando de este modo una hiperpolarización de membrana del organelo generador de energía.…”

Section: Discussionunclassified

Análisis Morfométrico del Efecto del Láser Infrarrojo sobre Hepatocitos de Rata

et al. 2009

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RESUMEN:El láser infrarrojo emitido por el diodo Arsenurio de Galio (904 nm) proporciona terapia a lesiones articulares por su acción analgésica, cicatrizante y antiinflamatoria, promoviendo a nivel celular síntesis de ATP mitocondrial, modulación de canales de calcio, activando el proceso mitótico e incremento en la síntesis de DNA y de proteínas. Para determinar las dosis que estimulen componentes celulares involucrados en síntesis proteica, del hígado de ratas fueron tomadas muestras de tejido normal e irradiado mediante láser infrarrojo con 1, 2, 4, 8 y 16 Joules/cm 2 durante 15 días consecutivos. Fueron tratadas para microscopía electrónica de transmisión y se obtuvieron micrografías con aumentos de 10.000 X. Se realizaron estudios morfométricos, cuantificándose las fracciones volumétricas de núcleos, citoplasma, retículo endoplasmático rugoso (RER), inclusiones de glicógeno, nucleolos, eucromatina y heterocromatina, relación núcleo-citoplasmática y las áreas celulares y nucleares. Los resultados del presente estudio que compara hepatocitos normales e irradiados, indican que existen diferencias significativas en todos los parámetros evaluados. Se concluye que los hepatocitos estimulados alteran su morfología y por ende sus componentes celulares, modificando la función celular determinándose con exactitud la dosis de estimulación infrarroja donde estas células presentan un mayor desarrollo de su maquinaria citoplasmática involucrada en síntesis de proteínas.

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The irradiation of hepatocytes with He‐Ne laser causes an increase of cytosolic free calcium concentration and an increase of cell membrane potential, correlated with it, both increases taking place in an oscillatory manner

Cited by 13 publications

References 8 publications

Mitochondrial Signaling in Mammalian Cells Activated by Red and Near‐IR Radiation

Mitochondrial Signaling in Mammalian Cells Activated by Red and Near‐IR Radiation

Second messengers mediating the proliferation and collagen synthesis of tenocytes induced by low-level laser irradiation

Análisis Morfométrico del Efecto del Láser Infrarrojo sobre Hepatocitos de Rata

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