The Iron-Uptake System of the Plant-Growth-Stimulating Pseudomonas Putida WCS358: Genetic Analysis and Properties and Structure Analysis of Its Siderophore

Hofstad, Gerard A. J. M. van der; Marugg, Joey D.; Nielander, H. B.; Megen, Ingrid van; Pelt, A. M. M. Van; Recourt, K.; Weisbeek, Peter

doi:10.1007/978-3-642-71652-2_39

Cited by 2 publications

(2 citation statements)

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“…Ferrichrome, ferrichrome A, rhodotorulic acid, ferric pseudobactin 358, and ferric pseudobactin 589 gave no response in our assay (data not shown), indicating that MAb 5D4-C3 binds very weakly, if at all, to these siderophores. Pseudobactin 358 and pseudobactin 589 have structures similar, but not identical, to pseudobactin from Pseudomonas strain B10 (15,20). We previously reported that MAb 5D4-C3 did not bind to several other siderophores produced by fluorescent pseudomonads (4).…”

Section: Resultsmentioning

confidence: 93%

A Method for Detection of Pseudobactin, the Siderophore Produced by a Plant-Growth-Promoting Pseudomonas Strain, in the Barley Rhizosphere

Buyer

Kratzke

Sikora

1993

Appl Environ Microbiol

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Detection in the rhizosphere of the siderophore produced by an inoculated microorganism is critical to determining the role of microbial iron chelators on plant growth promotion. We previously reported the development of monoclonal antibodies (MAb) to ferric pseudobactin, the siderophore of plant-growthpromoting Pseudomonas strain B10. One of these MAb reacted less strongly to pseudobactin than to ferric pseudobactin. The MAb reacted to Al(III), Cr(III), Cu(II), and Mn(II) complexes of pseudobactin at a level similar to the level at which it reacted to ferric pseudobactin and reacted less to the Zn(ll) complex, but these metals would make up only a small fraction of chelated pseudobactin in soil on the basis of relative abundance of metals and relative binding constants. Fourteen-day-old barley plants grown in limed and autoclaved soil were inoculated with 109 CFU of Pseudomonas strain Sml-3, a strain of Pseudomonas B10 Rif Nal' selected for enhanced colonization, and sampled 3 days later. Extraction and analysis of the roots and surrounding soil using the MAb in an immunoassay indicated a concentration of 3.5 x 1010 mol of ferric pseudobactin g-1 (wet weight). This is the first direct measurement of a pseudobactin siderophore in soil or rhizosphere samples.

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Section: Resultsmentioning

confidence: 93%

A Method for Detection of Pseudobactin, the Siderophore Produced by a Plant-Growth-Promoting Pseudomonas Strain, in the Barley Rhizosphere

Buyer

Kratzke

Sikora

1993

Appl Environ Microbiol

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“…are efficient competitors for ferric iron (5, 16, 24, 27, 29-31, 37, 38). Their siderophores, called pyoverdines or pseudobactins, have a high affinity for iron (29,40,42). Siderophore-mediated competition for iron was demonstrated or postulated to be responsible for suppression of disease development of several soilborne pathogens, including fusarium wilt, by strains of fluorescent Pseudomonas spp.…”

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confidence: 99%

Antagonistic Effect of Nonpathogenic Fusarium oxysporum Fo47 and Pseudobactin 358 upon Pathogenic Fusarium oxysporum f. sp. dianthi

Lemanceau

Bakker

Kogel

et al. 1993

Appl Environ Microbiol

127

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The antagonistic effect of Fo47blO and purified pseudobactin 358 was studied by using an in vitro bioassay. This bioassay allows studies on interactions among nonpathogenic F. oxysporum Fo47blO, pathogenic F. oxysporum f. sp. dianthi WCS816, and purified pseudobactin 358, the fluorescent siderophore produced by P. putida WCS358. Both nonpathogenic and pathogenic F. oxysporum reduced each other's growth when grown together. However, in these coinoculation experiments, pathogenic F. oxysporum WCS816 was relatively more inhibited in its growth than nonpathogenic F. oxysporum Fo47blO. The antagonism of nonpathogenic F. oxysporum against pathogenic F. oxysporum strongly depends on the ratio of nonpathogenic to pathogenic F. oxysporum densities: the higher this ratio, the stronger the antagonism. This fungal antagonism appears to be mainly associated with the competition for glucose. Pseudobactin 358 reduced the growth of both F. oxysporum strains, whereas ferric pseudobactin 358 did not; antagonism by pseudobactin 358 was then related to competition for iron. However, the pathogenic F. oxysporum strain was more sensitive to this antagonism than the nonpathogenic strain. Pseudobactin 358 reduced the efficiency of glucose metabolism by the fungi. These results suggest that pseudobactin 358 increases the intensity of the antagonism of nonpathogenic F. oxysporum Fo47blO against pathogenic F. oxysporum WCS816 by making WCS816 more sensitive to the glucose competition by Fo47blO.

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The Iron-Uptake System of the Plant-Growth-Stimulating Pseudomonas Putida WCS358: Genetic Analysis and Properties and Structure Analysis of Its Siderophore

Cited by 2 publications

References 4 publications

A Method for Detection of Pseudobactin, the Siderophore Produced by a Plant-Growth-Promoting Pseudomonas Strain, in the Barley Rhizosphere

A Method for Detection of Pseudobactin, the Siderophore Produced by a Plant-Growth-Promoting Pseudomonas Strain, in the Barley Rhizosphere

Antagonistic Effect of Nonpathogenic Fusarium oxysporum Fo47 and Pseudobactin 358 upon Pathogenic Fusarium oxysporum f. sp. dianthi

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