The iron-molybdenum cofactor of nitrogenase

Burgess, Barbara K.

doi:10.1021/cr00106a002

Cited by 354 publications

(262 citation statements)

References 57 publications

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“…The trapped molybdenum(VI) may then be taken up and converted to e.g. the iron-molybdenum cofactor of nitrogenase, in which molybdenum is coordinated in a different way (Burgess et al 1990). Although there are limitations with the EXAFS method as discussed above, our work confirms that molybdenum(VI) sorption to organic matter is important, and that it involves complexes with monomeric molybdenum(VI) in a distorted octahedral configuration.…”

Section: Discussionsupporting

confidence: 61%

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Molybdenum binding to soil constituents in acid soils: An XAS and modelling study

Gustafsson

Tiberg

2015

Chemical Geology

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Highlights:-Molybdenum(VI) is bound to ferrihydrite and aluminium hydroxide predominantly as tetrahedral, edge-sharing inner-sphere and outer-sphere complexes.-On natural organic matter, molybdenum(VI) is bound at low pH as an octahedrally coordinated monomeric complex.-Results for a spodic B horizon show an Al(OH) 3 -type phase to be an important sorbent for molybdenum(VI).-The results were used to suggest new complexation constants for the CD-MUSIC and Stockholm Humic models. 2 AbstractDespite its importance as a trace element, the binding mechanisms of molybdenum in soils are not well known. In this study, we studied the binding of molybdenum onto selected soil samples, and we used X-ray absorption spectroscopy (XAS) to characterise the coordination of molybdenum on three important environmental sorbents: ferrihydrite (Fh), amorphous aluminium hydroxide (Al(OH) 3 ) and fulvic acid. The X-ray near-edge structure (XANES) data showed that the added molybdenum(VI) was not reduced, although for the organic samples the coordination shifted from tetrahedral to octahedral. The EXAFS (extended X-ray absorption fine structure) analysis showed that molybdenum(VI) on Fh and Al(OH

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Section: Discussionsupporting

confidence: 61%

“…The Mo-containing enzyme nitrogenase catalyzes the reaction. In the most common form of this enzyme, molybdenum and iron are co-factors, where molybdenum is coordinated to a sum of about six oxygen and sulphur atoms in a distorted octahedral configuration (Burgess, 1990).…”

Section: Introductionmentioning

confidence: 99%

Molybdenum binding to soil constituents in acid soils: An XAS and modelling study

Gustafsson

Tiberg

2015

Chemical Geology

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“…Although many forms that contain alterations of the polypeptide in the FeMo cofactor pocket and those that have alterations in the homocitrate portion of FeMo cofactor have little or no N 2 fixation activity all proteins purified to date are able to reduce H ϩ to H 2 (12,33). As described in the Introduction, our goal was to construct a form that was similar enough to the wild-type MoFe protein that it could accept electrons from the Fe protein but that was not able to transfer those electrons to any substrate, including H ϩ .…”

Section: Resultsmentioning

confidence: 99%

Construction of a Form of the MoFe Protein of Nitrogenase That Accepts Electrons from the Fe Protein but Does Not Reduce Substrate

Ma¹,

Brosius²,

Burgess³

1996

Journal of Biological Chemistry

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The direction of electron flow through nitrogenase is generally believed to be from the Fe protein to the Pclusters to the FeMo cofactor and then to substrate. In order to examine oxidation states of the P-clusters that might be involved in this pathway, we have constructed a form of the MoFe protein that contains a species called the MoFe cluster Molybdenum nitrogenase is composed of two separate proteins whose complete structures have recently been determined by x-ray crystallography (1-7). The smaller of the two, designated the iron protein (Fe protein), is a dimer of two identical subunits encoded by the nifH gene (1, 8). It contains two binding sites for MgATP and has a single [4Fe-4S] 2ϩ/ϩ cluster. The larger of the two component proteins is designated the molybdenum-iron protein (MoFe protein) and is an a 2 ␤ 2 tetramer with the ␣ and ␤ subunits encoded by the nifD and nifK genes, respectively. It has two types of metal centers, the P-clusters (two per tetramer) that each contain 8Fe and 8S 2Ϫ atoms in the form of bridged [4Fe-4S] clusters and, the iron molybdenum cofactors (FeMo cofactor) (two per tetramer) that have the composition Mo:Fe 7

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“…Yuusuke KUBOTA, Kazutaka KOGA 1) , Masatomi OHNO and Tamio Fundamental chemical reactions between an atmospheric nitrogen plasma jet and water or a water/ethanol solution have been investigated. When the nitrogen plasma jet was injected directly into pure water, active nitrogen species produced nitric acid and ammonia through fatal reactions with H 2 O.…”

Section: Synthesis Of Ammonia Through Direct Chemical Reactions Betwementioning

confidence: 99%

“…These experimental results clearly show that the active nitrogen species in the pulsed-discharge atmospheric plasma can interact strongly with materials in solution to extract oxygen or hydrogen from them and add atomic nitrogen to them. Although ammonia (NH 3 ) is currently synthesized from nitrogen (N 2 ) and hydrogen (H 2 ) at high temperature (500 • C) and high pressure (above 200 atm) with iron catalysts, a biological nitrogen-fixation system can operate under mild conditions [1][2][3][4]. A few research groups reported nonbiological catalytic reduction of nitrogen to ammonia at ambient temperature and pressure with catalysts including metal centers [5][6][7][8][9].…”

Section: Synthesis Of Ammonia Through Direct Chemical Reactions Betwementioning

confidence: 99%

Synthesis of Ammonia through Direct Chemical Reactions between an Atmospheric Nitrogen Plasma Jet and a Liquid

Kubota

Koga

Ohno

et al. 2010

Plasma and Fusion Research

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Fundamental chemical reactions between an atmospheric nitrogen plasma jet and water or a water/ethanol solution have been investigated. When the nitrogen plasma jet was injected directly into pure water, active nitrogen species produced nitric acid and ammonia through fatal reactions with H 2 O. The interaction between nitrogen plasma and a liquid mixture of pure water and ethanol produced NH 4 + ions effectively. These experimental results clearly show that the active nitrogen species in the pulsed-discharge atmospheric plasma can interact strongly with materials in solution to extract oxygen or hydrogen from them and add atomic nitrogen to them.

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The iron-molybdenum cofactor of nitrogenase

Cited by 354 publications

References 57 publications

Molybdenum binding to soil constituents in acid soils: An XAS and modelling study

Molybdenum binding to soil constituents in acid soils: An XAS and modelling study

Construction of a Form of the MoFe Protein of Nitrogenase That Accepts Electrons from the Fe Protein but Does Not Reduce Substrate

Synthesis of Ammonia through Direct Chemical Reactions between an Atmospheric Nitrogen Plasma Jet and a Liquid

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