The IRG1-Itaconate Axis Promotes Viral Replication Via Metabolic Reprogramming and Protein Prenylation

Yin, Shulei; Han, Diana; Tao, Yulong; Xu, Sheng; Li, Tianliang; Li, Jiangxue; Li, Yingke; Huang, Jingyi; Cao, Xuetao; Yu, Yizhi

doi:10.2139/ssrn.3440261

Cited by 3 publications

(1 citation statement)

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“…In particular, while in some cases an over-expression of acod1 seemed to promote an antiviral action, in other studies involving vesicular stomatitis virus (VSV, another virus of the family Rhabdoviridae ) and human alphaherpesvirus 1 (family Herpesviridae ), acod1 was associated with the promotion of viral replication [ 51 ]. Moreover, it was also observed that the over-expression of acod1 in organs of mice infected with VSV favored the entry and overgrowth of this viral species by producing itaconate, which promoted protein prenylation via an IFN-I independent mechanism [ 52 ]. Acod1 was also found among the over-expressed genes in a recent study on RNA-Seq conducted on avian influenza virus (Family Orthomixoviridae ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Profiling of Oncorhynchus mykiss Infected with Low or Highly Pathogenic Viral Hemorrhagic Septicemia Virus (VHSV)

Biasini,

Zamperin,

Pascoli

et al. 2023

Microorganisms

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The rainbow trout (Oncorhynchus mykiss) is the most important produced species in freshwater within the European Union, usually reared in intensive farming systems. This species is highly susceptible to viral hemorrhagic septicemia (VHS), a severe systemic disease widespread globally throughout the world. Viral hemorrhagic septicemia virus (VHSV) is the etiological agent and, recently, three classes of VHSV virulence (high, moderate, and low) have been proposed based on the mortality rates, which are strictly dependent on the viral strain. The molecular mechanisms that regulate VHSV virulence and the stimulated gene responses in the host during infection are not completely unveiled. While some preliminary transcriptomic studies have been reported in other fish species, to date there are no publications on rainbow trout. Herein, we report the first time-course RNA sequencing analysis on rainbow trout juveniles experimentally infected with high and low VHSV pathogenic Italian strains. Transcriptome analysis was performed on head kidney samples collected at different time points (1, 2, and 5 days post infection). A large set of notable genes were found to be differentially expressed (DEGs) in all the challenged groups (e.s. trim63a, acod1, cox-2, skia, hipk1, cx35.4, ins, mtnr1a, tlr3, tlr7, mda5, lgp2). Moreover, the number of DEGs progressively increased especially during time with a greater amount found in the group infected with the high VHSV virulent strain. The gene ontology (GO) enrichment analysis highlighted that functions related to inflammation were modulated in rainbow trout during the first days of VHSV infection, regardless of the pathogenicity of the strain. While some functions showed slight differences in enrichments between the two infected groups, others appeared more exclusively modulated in the group challenged with the highly pathogenic strain.

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Section: Discussionmentioning

confidence: 99%