The involvement of osteopontin and β3 integrin in implantation and endometrial receptivity in an early mouse pregnancy model

Liu, Nenghui; Zhou, Can; Chen, Yuxiang; Zhao, Jingfeng

doi:10.1016/j.ejogrb.2013.06.019

Cited by 34 publications

(29 citation statements)

References 15 publications

(21 reference statements)

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“…Since in addition to the EECs, SPP1 receptors are expressed on the trophoblast cells, it was suggested that SPP1 serves as a bridge that links the luminal uterine cells with the blastocyst (Apparao et al 2001, Johnson et al 2003, thus playing a crucial role in establishing the embryo-endometrium interaction. Studies carried out in mice and rabbits indeed showed that using specific antibodies for functional blocking of endometrial, either ITGavb3 or SPP1 in vivo significantly reduced the number of implantation sites (Illera et al 2000, Liu et al 2013. Further in vitro experiments demonstrated that blastocysts failed to attach to EECs pre-treated with ITGb3 siRNA (Kaneko et al 2011).…”

Section: Cd11cmentioning

confidence: 99%

Biopsy-induced inflammatory conditions improve endometrial receptivity: the mechanism of action

Gnainsky¹,

Granot²,

Aldo³

et al. 2015

Reproduction

135

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A decade ago, we first reported that endometrial biopsy significantly improves the success of pregnancy in IVF patients with recurrent implantation failure, an observation that was later confirmed by others. Recently, we have demonstrated that this treatment elevated the levels of endometrial pro-inflammatory cytokines and increased the abundance of macrophages (Mac) and dendritic cells (DCs). We therefore hypothesised that the biopsy-related successful pregnancy is secondary to an inflammatory response, and aimed at deciphering its mechanism of action. Supporting our hypothesis, we found that the pro-inflammatory TNFa stimulated primary endometrial stromal cells to express cytokines that attracted monocytes and induced their differentiation into DCs. These monocytederived DCs stimulated endometrial epithelial cells to express the adhesive molecule SPP1 (osteopontin (OPN)) and its receptors ITGB3 and CD44, whereas MUC16, which interferes with adhesion, was downregulated. Other implantation-associated genes, such as CHST2, CCL4 (MIP1B) and GROA, were upregulated by monocyte-derived Mac. These findings suggest that uterine receptivity is mediated by the expression of molecules associated with inflammation. Such an inflammatory milieu is not generated in some IVF patients with recurrent implantation failure in the absence of local injury provoked by the biopsy treatment.Reproduction (2015) 149 75-85

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Section: Cd11cmentioning

confidence: 99%

Biopsy-induced inflammatory conditions improve endometrial receptivity: the mechanism of action

Gnainsky¹,

Granot²,

Aldo³

et al. 2015

Reproduction

135

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“…This implies an interaction between integrin αv and an extracellular bridging ligand (see poster) regarding attachment (Singh and Aplin, 2009). Both in vivo (Johnson et al, 2014;Liu et al, 2013) and in vitro evidence supports a role for osteopontin (OPN) in embryo attachment to epithelium. Another candidate is fibronectin, a specific glycoform of which is produced by trophectoderm (Kaneko et al, 2013;Shimomura et al, 2006;Turpeenniemi-Hujanen et al, 1995).…”

Section: Box 2: Demonstrating Integrin Function In Implantationmentioning

confidence: 99%

Embryo–epithelium interactions during implantation at a glance

Aplin

Ruane

2017

Journal of Cell Science

191

140

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At implantation, with the acquisition of a receptive phenotype in the uterine epithelium, an initial tenuous attachment of embryonic trophectoderm initiates reorganisation of epithelial polarity to enable stable embryo attachment and the differentiation of invasive trophoblasts. In this Cell Science at a Glance article, we describe cellular and molecular events during the epithelial phase of implantation in rodent, drawing on morphological studies both in vivo and in vitro, and genetic models. Evidence is emerging for a repertoire of transcription factors downstream of the master steroidal regulators estrogen and progesterone that coordinate alterations in epithelial polarity, delivery of signals to the stroma and epithelial cell death or displacement. We discuss what is known of the cell interactions that occur during implantation, before considering specific adhesion molecules. We compare the rodent data with our much more limited knowledge of the human system, where direct mechanistic evidence is hard to obtain. In the accompanying poster, we represent the embryo-epithelium interactions in humans and laboratory rodents, highlighting similarities and differences, as well as depict some of the key cell biological events that enable interstitial implantation to occur.

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“…However, expression of MUC1 in the nonpregnant mare has not been reported. Secreted phosphoprotein 1 (SPP1), also known as osteopontin, is an extracellular matrix protein whose differential regulation of expression during early pregnancy has been implicated in uterine receptivity in humans, pigs, mice, and sheep (Johnson et al 2014 ;Liu et al 2013 ;Qu et al 2008 ); in that regard, SPP1 is an extracellular matrix protein that mediates conceptus adhesion by bridging trophoblast and endometrial integrins. In the mare, there are limited studies characterizing expression and localization of SPP1 at the conceptus-maternal interface.…”

Section: Expression Of Proteins Related To Uterine Receptivity To Impmentioning

confidence: 99%

Pregnancy Recognition and Implantation of the Conceptus in the Mare

Klein

2015

Regulation of Implantation and Establishment of Pregnancy in Mammals

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Few, if any, biological processes are as diverse among domestic species as establishment of early pregnancy, in particular maternal recognition of pregnancy. Following fertilization and initial development in the mare oviduct, selective transport of the embryo through the uterotubal junction driven by embryo-derived PGE2 occurs. Upon arrival in the uterus, an acellular glycoprotein capsule is formed that covers the embryo, blastocyst, and conceptus (embryo and associated extraembryonic membranes) between the second and third weeks of pregnancy. Between Days 9 and 15/16 of pregnancy, the conceptus undergoes an extended phase of mobility. Conceptus mobility is driven by conceptus-derived PGF2α and PGE2 that stimulate uterine contractions which in turn propel migration of the conceptus within the uterine lumen. Cessation of conceptus mobility is referred to as fixation and appears to be attributable to increasing size of the conceptus, preferential thickening of the endometrium near the mesometrial attachment referred to as encroachment, and a reduction in sialic acid content of the capsule. During maternal recognition of pregnancy, endometrial PGF2α release is attenuated, a consequence of reduced expression of key enzymes involved in prostaglandin production. Oxytocin responsiveness is altered during early pregnancy, and reduced expression of the oxytocin receptor appears to be regulated at the posttranscriptional level rather than the transcriptional level. Prostaglandin release is attenuated temporarily only during early pregnancy; during the third week of pregnancy, the endometrium resumes the ability to secrete PGF2α. The equine conceptus initiates steroidogenesis as early as Day 6 and synthesizes estrogens, androgens, and progesterone. Estrogens are metabolized locally, presumably regulating their bioavailability and actions. Results of experiments attempting to prove that conceptus-derived estrogens are responsible for extension of corpus luteum function have been inconclusive. By the fourth week of pregnancy, the chorionic girdle becomes visible on the trophoblast. Subsequent invasion of chorionic girdle cells leads to formation of endometrial cups which secrete equine chorionic gonadotropin. Equine chorionic gonadotropin has luteinizing hormone functions in the mare, causing luteinization of follicles resulting in the formation of secondary corpora lutea essential to production of progesterone and maintenance of pregnancy.

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The involvement of osteopontin and β3 integrin in implantation and endometrial receptivity in an early mouse pregnancy model

Cited by 34 publications

References 15 publications

Biopsy-induced inflammatory conditions improve endometrial receptivity: the mechanism of action

Biopsy-induced inflammatory conditions improve endometrial receptivity: the mechanism of action

Embryo–epithelium interactions during implantation at a glance

Pregnancy Recognition and Implantation of the Conceptus in the Mare

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