The involvement of mitochondrial phosphate transporter in accelerating bud dormancy release during chilling treatment of tree peony (Paeonia suffruticosa)

Abstract

A cDNA clone was isolated from tree peony (Paeonia suffruticosa) subtractive cDNA library of burst buds and characterized with regard to its sequence, expression in response to chilling treatment during the release of bud dormancy, and its function in transgenic Arabidopsis thaliana. The clone, designated as PsMPT, contains 1,615 nucleotides with an open reading frame of 1,119 nucleotides, and the deduced amino acid sequence shows high homology with mitochondrial phosphate transporters (MPTs) from various organisms. The mRNA accumulation of PsMPT in tree peony was strongly induced by chilling treatment during the release of bud dormancy. When the treated plants were transferred to normal growth conditions, the level of PsMPT transcripts induced by sufficient chilling could be maintained high, whereas that induced by insufficient chilling decreased sharply. The transgenic Arabidopsis plants that overexpress PsMPT showed rapid growth and earlier flowering than wild-type plants. ATP contents in the transgenic plants were much higher than that in wild-type plants through various developmental stages. Together, these results suggest that the product of PsMPT is a MPT and might play an important role during the release of bud dormancy in tree peony.

Transcriptome analysis of tree peony during chilling requirement fulfillment: Assembling, annotation and markers discovering

“…Comparing with other fruit plants, the knowledge about dormancy release in tree peony is relatively limited. Huang et al reported 31 unique genes that might be associated with dormancy release during chilling treatments in a subtractive cDNA library, but most of them were related to structural function (Huang et al, 2008a(Huang et al, , 2008b. Currently only 2204 pieces of EST are found in National Center for Biotechnology Information (NCBI) database, and most of them were recently submitted with a purpose for bud differentiation in tree peony (Shu et al, 2009).…”

Cloning and expression analysis of the R2R3-PsMYB1 gene associated with bud dormancy during chilling treatment in the tree peony (Paeonia suffruticosa)

“…c Northern blotting analysis of tree peony PsMYB1 expression after 0, 7, 14, 21 and 28 days chilling treatment. d Expression analysis of PsMYB1 in the tree peony flower buds enduring 7 and 21 days chilling treatments and then transferred to the greenhouse using qPCR Plant Growth Regul and Gai et al screened different stages during endo-dormancy release (Huang et al 2008b;Gai et al 2013). Based on morphological changes and global mRNA profiling of differentially expressed genes, they found that flower buds that received less than 18 days chilling treatment were regarded as endo-dormancy in physiological status, while those that received more than 18 days of chilling treatment were regarded as eco-dormancy.…”

Expression, transcriptional regulation and functional analysis of phosphate transporter genes in plants

“…In petunia corollas, a high-affinity phosphate transporter gene PhPT1 (PhPht1;1), which shows to be up-regulated during senescence and possibly to be functional in Pi translocation during senescence, shows a rapid increase followed with low levels of ethylene treatment (0.1 mM) (Chapin and Jones, 2009). The mRNA accumulation of PsMPT in tree peony was strongly induced by chilling treatment during the release of bud dormancy (Huang et al, 2008). Together, these results imply that the plant Pi transporter genes have a complicated transcriptional mechanism regulated by many environmental factors, which include Pi, Zn and other unknown inorganic nutrients, and other factors.…”

“…Mpt1 contains an open reading frame of 1092 bases encoding a 364 amino acid polypeptide, much fewer than those in cytosol membrane and plastid envelope (Kiiskinen et al, 1997). Other mitochondrial phosphate transporter genes have also been identified in soybean, maize, rice, and Arabidopsis (Takabatake et al, 1999), Lotus japonicus (Nakamori et al, 2002), and tree peony (Paeonia suffruticosa) (Huang et al, 2008). It is currently known that most of the mitochondrial phosphate transporters in plants have generally about six of the conserved transmembrane domains, and nearly half of those targeted in cytosol membrane and plastid envelope.…”

“…Huang et al (2008) observed that a tree peony Pi transporter, PsMPT, also plays an important role in the release of ATP during bud dormancy. In Arabidopsis, overexpression of a high-affinity Pi transporter PHT1;1 results in a slow rate of arsenic (As(V)) uptake in the transgenic plants, suggesting also that part of genes specifically involved in Pi uptake are transcriptionally regulated by As(V) (Catarecha et al, 2007).…”