The involvement of low-molecular antioxidants in cross-adaptation of medicine plants to successive action of UV-B radiation and salinity

Abstract

Artemisia (Artemisia lercheana Web.), common basil (Ocimum basilicum L.), and black cumin (Nigella sativa L.) plants grown in water culture until the stage of 4-5 true leaves were subjected to 10 min UV B irradiation, treated with 100 mM NaCl, or subjected to the successive action of both stressors. The contents of proline, anthocyanins, flavonoids, soluble phenols, and carotenois were measured. Superoxide dismutase activity was also assayed. Experimental plants could tolerate UV B irradiation due to the accumu lation of phenolic compounds (anthocyanins, soluble phenols, and flavonoids). Anthocyanins contributed mostly in the defnse effect; their content in black cumin and common basil increased 3-5 fold after irradia tion. Dynamics of the anthocyanin content in tested plants of all treatments indicates the activation of their biosynthesis by UV B irradiation and suppression by salinity. Successive action of stressors resulted in syner gism of their effects on accumulation of low molecular compounds in artemisia and common cumin plants. When these plants were irradiated with UV B and then treated with NaCl, they accumulated more low molecular compounds than after separate treatment with these stressors, especially in Artemisia. Plant pre treatment with UV B reduced the adverse action of salinity; this was manifested in the turgor maintenance in salt treated plants after preliminary irradiation. For basil, salinity was the stronger stressor than UV B, which was manifested in a decrease in the content of low molecular compounds.

Visual Quality and Morphological Responses of Rosemary Plants to UV-B Radiation and Salinity Stress

“…(Dubé & Bornman, 1992;Hofmann et al, 2003). Some studies suggest that plants could benefit from cross-tolerance when salinity and ultraviolet are applied simultaneously (Fedina et al,2003;Radyukina et al,2012;Ouhibi et al,2014).…”

Phenolic compounds in plants: biogenesis and functions

“…The protective effect of PCs during UV irradiation also based on the interaction with peroxides and free radicals that leads to the quenching of free radical reactions occurring in cells affected by UV radiation [58]. The accumulation of anthocyanins in the medicinal plants ocimum basilicum, artemisia lercheana and Nigella sativa in response to UV action, salinity and the combined effect of these two factors has been reported [61]. It is interesting that the stress-induced increase in anthocyanin content in Nigella sativa was many times greater than the corresponding values in ocimum basilicum and artemisia lercheana.…”

“…It is interesting that the stress-induced increase in anthocyanin content in Nigella sativa was many times greater than the corresponding values in ocimum basilicum and artemisia lercheana. It was established that in plants that accumulate a large number of low molecular weight antioxidants, changes in the activity of antioxidant enzymes were less pronounced [61].…”

Hydrogen Sulfide Donor Influence on State of Antioxidant System of Wheat Seedlings Under Osmotic Stress

“…Не виключено, що ці низькомолекулярні антиоксиданти за таких експериментальних умов до певної міри компенсують відсутність істотного зростання активності СОД. У літературі є відомості про характерне для деяких видів рослин накопичення за стресових умов низькомолекулярних протекторів (флавоноїдів, проліну) за відсутності помітного зростання активності антиоксидантних ферментів [30]. Проте питання стосовно механізмів функціональної взаємодії антиоксидантів виходить за межі нашого дослідження.…”