The involvement of genetic polymorphism of IL-10 promoter in non-small cell lung cancer

Shih, Chuen-Ming; Lee, Yao-Ling; Chiou, Hui‐Ling; Hsu, Wei-Fang; Chen, Wei-Erh; Chou, Ming‐Chih; Lin, Long-Yau

doi:10.1016/j.lungcan.2005.07.007

Cited by 77 publications

(73 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…45 Two units of each restriction enzyme were used in DNA digestion. For −1082 SNP, we digested PCR product with MnlI (Vivantis, Selangor Darul Ehsan, Malaysia) at 37°C for 3 h and resulted in 106 bp, and 33 bp with allele −1082G and 139 bp with allele −1082A.…”

Section: Dna Extraction and Genotyping Of Il-10 By Polymerase Chain Rmentioning

confidence: 99%

Inflammatory breast cancer: High incidence of GCC haplotypes (−1082A/G, −819T/C, and −592A/C) in the interleukin-10 gene promoter correlates with over-expression of interleukin-10 in patients’ carcinoma tissues

et al. 2017

View full text Add to dashboard Cite

Interleukin-10 is involved in carcinogenesis by supporting tumor escape from the immune response. The aim of this study was to assess the single nucleotide polymorphisms, −1082A/G, −819T/C and −592A/C, in interleukin-10 gene promoter in inflammatory breast cancer compared to non-inflammatory breast cancer and association of these polymorphisms with interleukin-10 gene expression. We enrolled 105 breast cancer tissue (72 non-inflammatory breast cancer and 33 inflammatory breast cancer) patients and we determined the three studied single nucleotide polymorphisms in all samples by polymerase chain reaction restriction fragment length polymorphism and investigated their association with the disease and with various prognostic factors. In addition, we assessed the expression of interleukin-10 gene by realtime quantitative reverse transcription polymerase chain reaction and the correlation between studied single nucleotide polymorphisms and interleukin-10 messenger RNA expression. We found co-dominant effect as the best inheritance model (in the three studied single nucleotide polymorphisms in non-inflammatory breast cancer and inflammatory breast cancer samples), and we didn't identify any association between single nucleotide polymorphisms genotypes and breast cancer prognostic factors. However, GCC haplotype was found highly associated with inflammatory breast cancer risk (p < 0.001, odds ratio = 43.05). Moreover, the expression of interleukin-10 messenger RNA was significantly higher (p < 0.001) by 5.28-fold and 8.95-fold than non-inflammatory breast cancer and healthy control, respectively, where GCC haplotype significantly increased interleukin-10 gene expression (r = 0.9, p < 0.001).

show abstract

Section: Dna Extraction and Genotyping Of Il-10 By Polymerase Chain Rmentioning

confidence: 99%

Inflammatory breast cancer: High incidence of GCC haplotypes (−1082A/G, −819T/C, and −592A/C) in the interleukin-10 gene promoter correlates with over-expression of interleukin-10 in patients’ carcinoma tissues

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Cytokine gene polymorphisms in IL-1β (-511 and +3954) and IL-10 (-1082 and -592) were examined by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis, as described previously [26,27] and visualized by 50 mL/L ethidium bromide staining on 30 mL/L agarose gels. The IL-1RN variable number of tandem repeat (VNTR) polymorphism was detected by PCR and visualized on 20 mL/L agarose gels with alleles being classified conventionally according to El-Omar et al [18] as follows: allele 1, four repeats; allele 2, two repeats; allele 3, five repeats; allele 4, three repeats and allele 5, six repeats.…”

Section: Extraction Of Human Dna and Genotyping Of Cytokine Polymorphmentioning

confidence: 99%

Role of bacterial and genetic factors in gastric cancer in Costa Rica

Con¹,

Takeuchi²,

Con-Chin³

et al. 2009

WJG

View full text Add to dashboard Cite

AIM:To evaluate several risk factors for gastric cancer (GC) in Costa Rican regions with contrasting GC incidence rate (GCIR). METHODS:According to GCIR, 191 Helicobacter pylori (H pylori )-positive patients were classified into groups A (high GCIR, n = 101) and B (low GCIR, n = 90). Human DNA obtained from biopsy specimens was used in the determination of polymorphisms of the genes coding for interleukin (IL)-1β and IL-10 by PCR-RFLP, and IL-1RN by PCR. H pylori DNA extractions obtained from clinical isolates of 83 patients were used for PCR-based genotyping of H pylori cagA , vacA and babA2 . Human DNA from gastric biopsies of 52 GC patients was utilized for comparative purposes. RESULTS:Cytokine polymorphisms showed no association with GCIR variability. However, gastric atrophy, intestinal metaplasia and strains with different vacA genotypes in the same stomach (mixed strain infection) were more frequently found in group A than in group B, and cagA and vacA s1b were significantly associated with high GCIR (P = 0.026 and 0.041, respectively). IL-1β+3954_T/C (OR 2.1, 1.0-4.3), IL-1RN*2/L (OR 3.5, 1.7-7.3) and IL-10-592_C/A (OR 3.2, 1.5-6.8) were individually associated with GC, and a combination of these cytokine polymorphisms with H pylori vacA s1b and m1 further increased the risk (OR 7.2,. C O N C L U S I O N :A l t h o u g h a p r o i n f l a m m a t o r y cytokine genetic profile showed an increased risk for developing GC, the characteristics of H pylori infection, in particular the status of cagA and vacA genotype distribution seemed to play a major role in GCIR variability in Costa Rica.

show abstract

“…[12][13][14], IL1 receptor antagonist (IL1RN; refs. 15,16), IL6 (13,17), IL10 (18), cyclooxygenase 2 (17), and tumor necrosis factor a (19). These studies have yielded mixed results, and some were limited by small sample size.…”

Section: Introductionmentioning

confidence: 99%

Systematic Evaluation of Genetic Variants in the Inflammation Pathway and Risk of Lung Cancer

et al. 2007

View full text Add to dashboard Cite

Inflammatory responses to environmental exposures, such as tobacco smoke, may play a role in lung carcinogenesis. To test this hypothesis, we studied genetic polymorphisms in the inflammation pathway in relation to lung cancer risk. We evaluated a panel of 59 single nucleotide polymorphisms (SNP) in 37 inflammation-related genes among non-Hispanic Caucasian lung cancer cases (N = 1,553) and controls (N = 1,730) from Houston, Texas. Logistic regression was used to assess associations with lung cancer under a dominant genetic model adjusted for sex, age, and smoking. Haplotypes were estimated with the expectation-maximization algorithm. False-positive report probabilities (FPRP) were calculated for significant associations. Interleukin 1B (IL1B) C3954T was associated with lung cancer [odds ratio (OR), 1.27; 95% confidence interval (95% CI), 1.10-1.47; FPRP 0.148]. Two IL1A SNPs (C-889T and Ala 114 Ser) were also related to lung cancer (OR, 1.18-1.22), although FPRPs were higher. One IL1A-IL1B haplotype, containing only the IL1B 3954T allele, was associated with elevated lung cancer risk (OR, 1.80; 95% CI, 1.24-2.61). These associations were stronger in heavy smokers, particularly for IL1B C3954T (OR, 1.59; 95% CI, 1.28-1.97; FPRP 0.004). Lung cancer risk was unrelated to polymorphisms in IL1 receptor or antagonist genes. Associations with lung cancer were also seen for SNPs in granulocyte macrophage colony stimulating factor and peroxisome proliferatoractivated factor-D, but FPRPs were high. IL1A and IL1B polymorphisms are associated with increased lung cancer risk, especially among heavy smokers. IL1A and IL1B are critical signals in initiating inflammation. Our results suggest that a dysregulated inflammatory response to tobaccoinduced lung damage promotes carcinogenesis. [Cancer Res 2007;67(13):6520-7]

show abstract

The involvement of genetic polymorphism of IL-10 promoter in non-small cell lung cancer

Cited by 77 publications

References 32 publications

Inflammatory breast cancer: High incidence of GCC haplotypes (−1082A/G, −819T/C, and −592A/C) in the interleukin-10 gene promoter correlates with over-expression of interleukin-10 in patients’ carcinoma tissues

Inflammatory breast cancer: High incidence of GCC haplotypes (−1082A/G, −819T/C, and −592A/C) in the interleukin-10 gene promoter correlates with over-expression of interleukin-10 in patients’ carcinoma tissues

Role of bacterial and genetic factors in gastric cancer in Costa Rica

Systematic Evaluation of Genetic Variants in the Inflammation Pathway and Risk of Lung Cancer

Contact Info

Product

Resources

About