The involvement of extracellular vesicles in the transcytosis of nanoliposomes through brain endothelial cells, and the impact of liposomal pH-sensitivity

Recent Progress in Extracellular Vesicle-Based Carriers for Targeted Drug Delivery in Cancer Therapy

“…He et al, constructed an EV-mediated self-propelled liposome through membrane fusion liposomes, which maintained the structural integrity and stability of EVs and transferred fused lipids with functionalization directly to the plasma membrane of the recipient cells, achieving therapeutic or targeting effects on cancer cells in a more effective and controlled manner [ 96 ]. Reginald-Opara et al, investigated the effect of pH on liposome transcytosis and found that non-pH-sensitive liposomes could use the EVs secretion pathway to cross the blood–brain barrier for their brain endothelial cell transcytosis, whereas pH-sensitive liposomes facilitated cytoplasmic delivery [ 97 ].…”

Editorial of Special Issue “Cytoplasmic Delivery of Bioactives”

“…It has been reported that internalized mRNA-loaded lipid nanoparticles were re-packaged into extracellular vesicles (exosomes) and secreted from the recipient cells ( 25 ). Recent research found that the pH-sensitivity of liposomes increased cytoplasmic delivery and suppressed the degree of exocytosis of liposomes, as well as the transcytosis through an in vitro brain vascular endothelial monolayer model ( 26 ). Therefore, this Special Issue may also help open up new discussion avenues around these important topics.…”