The involvement of exosomes in the diagnosis and treatment of pancreatic cancer

Gabriel, Abakundana Nsenga Ariston; Wang, Fang; Jiao, Qinlian; Umwali, Yvette; Yang, Xuemei; Al-Ameri, Samed Ahmed Al-Ezzi; Li, Du; Wang, Yunshan; Wang, Chuanxin

doi:10.1186/s12943-020-01245-y

Cited by 58 publications

(38 citation statements)

References 100 publications

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“…They are secreted from almost all cell types, although tumor cells secrete more EVs compared to normal cells, playing an essential role in inducing metastasis and remodeling the primary microenvironment. Thus, it has been shown that EVs from fibroblasts promote invasive and chemoresistant behaviors in cancer cells [44,45], whereas EVs from tumor cells can reprogram fibroblasts into CAFs. In addition, it has been described that an increased amount of protein per exosomes predict disease progression [46,47].…”

Section: Discussionmentioning

confidence: 99%

Tumor–Stromal Interactions in a Co-Culture Model of Human Pancreatic Adenocarcinoma Cells and Fibroblasts and Their Connection with Tumor Spread

et al. 2021

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One key feature of pancreatic ductal adenocarcinoma (PDAC) is a dense desmoplastic reaction that has been recognized as playing important roles in metastasis and therapeutic resistance. We aim to study tumor–stromal interactions in an in vitro coculture model between human PDAC cells (Capan-1 or PL-45) and fibroblasts (LC5). Confocal immunofluorescence, Enzyme-Linked Immunosorbent Assay (ELISA), and Western blotting were used to evaluate the expressions of activation markers; cytokines arrays were performed to identify secretome profiles associated with migratory and invasive properties of tumor cells; extracellular vesicle production was examined by ELISA and transmission electron microscopy. Coculture conditions increased FGF-7 secretion and α-SMA expression, characterized by fibroblast activation and decreased epithelial marker E-cadherin in tumor cells. Interestingly, tumor cells and fibroblasts migrate together, with tumor cells in forming a center surrounded by fibroblasts, maximizing the contact between cells. We show a different mechanism for tumor spread through a cooperative migration between tumor cells and activated fibroblasts. Furthermore, IL-6 levels change significantly in coculture conditions, and this could affect the invasive and migratory capacities of cells. Targeting the interaction between tumor cells and the tumor microenvironment might represent a novel therapeutic approach to advanced PDAC.

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Section: Discussionmentioning

confidence: 99%

Tumor–Stromal Interactions in a Co-Culture Model of Human Pancreatic Adenocarcinoma Cells and Fibroblasts and Their Connection with Tumor Spread

et al. 2021

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“…in case of pancreatic cancer have also been reported by several studies. Other ncRNAs accounting for exosomal long noncoding RNA, circular RNA have also been reported pertaining to the development of exosomal biomarkers for early cancer detection [38,[50][51][52].…”

Section: Exosomes In Diagnosismentioning

confidence: 99%

CRISPR Cas/Exosome Based Diagnostics: Future of Early Cancer Detection

Mubthasima¹,

Pande²,

Prakash³

et al. 2022

Rural Health

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Trending and Thriving, CRISPR/Cas has expanded its wings towards diagnostics in recent years. The potential of evading off targeting has not only made CRISPR/Cas an effective therapeutic aid but also an impressive diagnostic tool for various pathological conditions. Exosomes, 30 - 150nm sized extracellular vesicle present and secreted by almost all type of cells in body per se used as an effective diagnostic tool in early cancer detection. Cancer being the leading cause of global morbidity and mortality can be effectively targeted if detected in the early stage, but most of the currently used diagnostic tool fails to do so as they can only detect the cancer in the later stage. This can be overcome by the use of combo of the two fore mentioned diagnostic aids, CRISPR/Cas alongside exosomes, which can bridge the gap compensating the cons. This chapter focus on two plausible use of CRISPR/Cas, one being the combinatorial aid of CRISPR/Cas and Exosome, the two substantial diagnostic tools for successfully combating cancer and other, the use of CRISPR in detecting and targeting cancer exosomes, since they are released in a significant quantity in early stage by the cancer cells.

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“…A growing body of research findings suggests that exosomes can be used for treating pancreatic cancer in the clinic for disease stabilization or reduction of aggressiveness [ 180 ]. The use of exosomes has been drawing intense attention because of their capability to transfer diverse biomolecules, including miRNAs, that may modulate the tumor microenvironment in an interactive fashion [ 180 ].…”

Section: Exosomal Mirnas In the Treatment Of Pdacmentioning

confidence: 99%

Exosomal microRNA in Pancreatic Cancer Diagnosis, Prognosis, and Treatment: From Bench to Bedside

Uddin

Hallak

Philip

et al. 2021

Cancers

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Pancreatic cancer is the fourth leading cause of cancer death among men and women in the United States, and pancreatic ductal adenocarcinoma (PDAC) accounts for more than 90% of pancreatic cancer cases. PDAC is one of the most lethal gastrointestinal malignancies with an overall five-year survival rate of ~10%. Developing effective therapeutic strategies against pancreatic cancer is a great challenge. Novel diagnostic, prognostic, and therapeutic strategies are an immediate necessity to increase the survival of pancreatic cancer patients. So far, studies have demonstrated microRNAs (miRNAs) as sensitive biomarkers because of their significant correlation with disease development and metastasis. The miRNAs have been shown to be more stable inside membrane-bound vesicles in the extracellular environment called exosomes. Varieties of miRNAs are released into the body fluids via exosomes depending on the normal physiological or pathological conditions of the body. In this review, we discuss the recent findings on the diagnostic, prognostic, and therapeutic roles of exosomal miRNAs in pancreatic cancer.

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The involvement of exosomes in the diagnosis and treatment of pancreatic cancer

Cited by 58 publications

References 100 publications

Tumor–Stromal Interactions in a Co-Culture Model of Human Pancreatic Adenocarcinoma Cells and Fibroblasts and Their Connection with Tumor Spread

Tumor–Stromal Interactions in a Co-Culture Model of Human Pancreatic Adenocarcinoma Cells and Fibroblasts and Their Connection with Tumor Spread

CRISPR Cas/Exosome Based Diagnostics: Future of Early Cancer Detection

Exosomal microRNA in Pancreatic Cancer Diagnosis, Prognosis, and Treatment: From Bench to Bedside

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