The Involvement of Corin in the Progression of Diabetic Erectile Dysfunction in a Rat Model by Down-Regulating ANP /NO/cGMP Signal Pathway

Abstract

This study was aimed to analyze the potential role of Corin in the procession of diabetic ED and to explore the underlying mechanism. Diabetic ED rat model was constructed and the characteristics of diabetic ED and control rats were recorded at 4, 8, 12, and 16 weeks. qRT-PCR and Western bloting were used to detected the mRNA and protein levels. Intracellular cGMP detection was accomplished using a commercial radioimmunoassay method. Vascular endothelial cell from rat corpus cavernosum spiral artery was isolated and transfected with si- Corin to analyzed the potential role of Corin. Cell viability was assessed using crystal violet. The results showed that diabetic ED rats showed significantly higher glucose level, and lower body weight, ICP level, and ICP/MAP ratio at 12 and 16 weeks in diabetic ED rats compared with control rats. The protein levels of Corin, atrial natriuretic peptide (ANP) and eNOS, and the level of cGMP were significantly down-regulated in corpus cavernosum in diabetic ED rats, revealing the potential role of Corin in NO-associated diabetic ED. Further, studies proved that defect of Corin not only inhibited the vascular endothelial cell viability in high-glucose condition, but also suppressed ANP, eNOS, and cGMP expression in vascular endothelial cells. To sum up, Corin contributes to the progression of diabetic ED and the underlying mechanism is associated with the down-regulation of ANP /NO/cGMP signal pathway. J. Cell. Biochem. 118: 2325-2332, 2017. © 2017 Wiley Periodicals, Inc.

Corin plays a protective role via upregulating MAPK and downregulating eNOS in diabetic nephropathy endothelial dysfunction

“…21 We showed that a lack of Corin in HK-2 cells could attenuate endothelial migration of EA.hy926 cells through paracrine crosstalk that is likely mediated by ANP. 12 It is plausible that impaired Corin-ANP signaling in diabetes is a common mechanism leading to endothelial dysfunction and subsequent diabetic microvascular complications. 11 Consistently, Jian Wang et al demonstrated that a lack of Corin could inhibit vascular endothelial cell viability.…”

“…11 Consistently, Jian Wang et al demonstrated that a lack of Corin could inhibit vascular endothelial cell viability. 12 It is plausible that impaired Corin-ANP signaling in diabetes is a common mechanism leading to endothelial dysfunction and subsequent diabetic microvascular complications.…”

“…14,15 Our previous studies demonstrated that Corin regulates spiral artery remodeling in the uterus during pregnancy 7 and affects endothelial dysfunction and vascular remodeling in DCM. 12 Thus, we hypothesize that Corin may play a pathogenetic role in DN by regulating endothelial function. Furthermore, a correlation between Corin and eNOS has been reported.…”

“…Furthermore, a correlation between Corin and eNOS has been reported. 12 Thus, we hypothesize that Corin may play a pathogenetic role in DN by regulating endothelial function.…”

“…Studies have shown reduced Corin expression in proteinuric kidney diseases and chronic kidney disease (CKD). 12,13 However, it is unknown whether nephric Corin is involved in the pathogenesis of DN. 11 Other studies also showed reduced Corin expression in diabetic erectile dysfunction and diabetic cardiovascular complications.…”