The involvement of bacterial quorum sensing in the spoilage of refrigerated Litopenaeus vannamei

Zhu, Shumin; Wu, Haohao; Zeng, Mingyong; Liu, Zunying; Wang, Ying

doi:10.1016/j.ijfoodmicro.2014.09.029

Cited by 74 publications

(95 citation statements)

References 29 publications

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“…In most Gram-negative bacteria, Quorum sensing is mediated by AHL molecules [4]. The trend in AHLs activity was consistent with the growth of H. alvei H4 within the first 18 h of incubation (Figure 6), since at late log phase, quorum sensing may play a more central role in bacterial physiology by slowing down its growth rate.…”

Section: Discussionsupporting

confidence: 64%

See 1 more Smart Citation

Characteristics of N-Acylhomoserine Lactones Produced by Hafnia alvei H4 Isolated from Spoiled Instant Sea Cucumber

Hou

Zhu

Wang

et al. 2017

Sensors

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This study aimed to identify N-acylhomoserine lactone (AHL) produced by Hafnia alvei H4, which was isolated from spoiled instant sea cucumber, and to investigate the effect of AHLs on biofilm formation. Two biosensor strains, Chromobacterium violaceum CV026 and Agrobacterium tumefaciens KYC55, were used to detect the quorum sensing (QS) activity of H. alvei H4 and to confirm the existence of AHL-mediated QS system. Thin layer chromatography (TLC) and high resolution triple quadrupole liquid chromatography/mass spectrometry (LC/MS) analysis of the AHLs extracted from the culture supernatant of H. alvei H4 revealed the existence of at least three AHLs: N-hexanoyl-l-homoserine lactone (C6-HSL), N-(3-oxo-octanoyl)-l-homoserine lactone (3-oxo-C8-HSL), and N-butyryl-l-homoserine lactone (C4-HSL). This is the first report of the production of C4-HSL by H. alvei. In order to determine the relationship between the production of AHL by H. alvei H4 and bacterial growth, the β-galactosidase assay was employed to monitor AHL activity during a 48-h growth phase. AHLs production reached a maximum level of 134.6 Miller unites at late log phase (after 18 h) and then decreased to a stable level of about 100 Miller unites. AHL production and bacterial growth displayed a similar trend, suggesting that growth of H. alvei H4 might be regulated by QS. The effect of AHLs on biofilm formation of H. alvei H4 was investigated by adding exogenous AHLs (C4-HSL, C6-HSL and 3-oxo-C8-HSL) to H. alvei H4 culture. Biofilm formation was significantly promoted (p < 0.05) by 5 and 10 µM C6-HSL, inhibited (p < 0.05) by C4-HSL (5 and 10 µM) and 5 µM 3-oxo-C8-HSL, suggesting that QS may have a regulatory role in the biofilm formation of H. alvei H4.

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Section: Discussionsupporting

confidence: 64%

“…Moreover, food spoilage is mostly caused by bacteria, and quorum sensing (QS) is thought to play a role in bacterial-linked food spoilage and food safety [2,3,4]. Increased interest in the connection between QS and food spoilage has led to more in-depth study of the signal molecules in relation to microbes that cause food spoilage.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of N-Acylhomoserine Lactones Produced by Hafnia alvei H4 Isolated from Spoiled Instant Sea Cucumber

Hou

Zhu

Wang

et al. 2017

Sensors

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“…QS has been reported to be involved in food spoilage, i.e., some specific spoilage organisms may produce an autoinducer to regulate their collective behavior leading to food spoilage, or bacterial species may sense the autoinducer secreted by others to monitor the density change (Zhu et al, 2015). AHLs are the most common autoinducers utilized by Gram-negative bacteria to regulate various biological functions.…”

Section: Discussionmentioning

confidence: 99%

“…The organic phase was collected and evaporated to dryness at 30°C. The dried extracts were further dissolved in 1.0 mL DMSO and stored at -20°C for further use (Zhu et al, 2015).…”

Section: Identification Of Ahls Assaysmentioning

confidence: 99%

Characterization of <i>N</i>-acyl homoserine lactones (AHLs) producing bacteria isolated from vacuum-packaged refrigerated turbot (<i>Scophthalmus maximus</i>) and possible influence of exogenous AHLs on bacterial phenotype

Zhang

Zhu

Jatt

et al. 2016

J. Gen. Appl. Microbiol.

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Quorum sensing (QS) is a cell-to-cell communication mechanism through which microbial cells communicate and regulate their wide variety of biological activities. N-acyl homoserine lactones (AHLs) are considered to be the most important QS signaling molecules produced by several Gramnegative bacteria. The present study aimed to screen the AHLs-producing bacteria from spoiled vacuum-packaged refrigerated turbot (Scophthalmus maximus) by biosensor assays, and the profiles of AHLs produced by these bacteria were determined using reversed-phase thin-layer chromatography (RP-TLC) and gas chromatography-mass spectrometry (GC-MS). Effects of exogenous AHLs and QS inhibitor (QSI) on the phenotypes (i.e., extracellular proteolytic activity and biofilm formation) of the AHLs-producing bacteria were also evaluated. Our results demonstrated that eight out of twenty-two isolates were found to produce AHLs. Three of the AHLs-producing isolates were identified as Serratia sp., and the other five were found to belong to the family of Aeromonas. Two isolates (i.e., S. liquefaciens A2 and A. sobria B1) with higher AHLs-producing activities were selected for further studies. Mainly, RP-TLC and GC-MS analysis revealed three AHLs, i.e., 3-oxo-C6-HSL, C8-HSL and C10-HSL were produced by S. liquefaciens A2, while five AHLs, i.e., C4-HSL, C6-HSL, C8-HSL, C10-HSL, and C12-HSL, were produced by A. sobria B1. Moreover, production of AHLs in both bacterial strains were found to be density-dependent, and the AHLs activity reached a maximum level in their middle logarithmic phase and decreased in the stationary phase. The addition of exogenous AHLs and QSI decreased the specific protease activity both of the Serratia A2 and Aeromonas B1. Exogenous AHLs inhibited the biofilm formation of Serratia A2 while it enhanced the biofilm formation in Aeromonas B1. QSI inhibited the specific protease activity and biofilm formation in both bacterial strains.

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“…The Pacific white shrimp, Litopenaeus vannamei, is a major farmed shrimp species, which accounts for more than one third of shrimp production in the world [1]. It is distributed along the Pacific coast from the northern Peru to the Gulf of California [2].…”

Section: Introductionmentioning

confidence: 99%

Daxx from Pacific white shrimp Litopenaeus vannamei is involved in activation of NF-κB pathway

Yan

Tang

Liang

et al. 2015

Fish & Shellfish Immunology

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The involvement of bacterial quorum sensing in the spoilage of refrigerated Litopenaeus vannamei

Cited by 74 publications

References 29 publications

Characteristics of N-Acylhomoserine Lactones Produced by Hafnia alvei H4 Isolated from Spoiled Instant Sea Cucumber

Characteristics of N-Acylhomoserine Lactones Produced by Hafnia alvei H4 Isolated from Spoiled Instant Sea Cucumber

Characterization of <i>N</i>-acyl homoserine lactones (AHLs) producing bacteria isolated from vacuum-packaged refrigerated turbot (<i>Scophthalmus maximus</i>) and possible influence of exogenous AHLs on bacterial phenotype

Daxx from Pacific white shrimp Litopenaeus vannamei is involved in activation of NF-κB pathway

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