The Involvement of Autoimmune Mechanism in Cystic and Inactive Ovaries of Dromedary Camel

Abstract

Numerous researchers have proposed work in dromedary camel immunity. It has not before been described how autoimmune etiology is involved in cystic and inactive camel ovaries. To specifically identify ELISA technique to measure anti-ovarian antibodies (Anti-OAB) in the serum, sixty female dromedary camels with various cysts and inactive ovaries during the breeding season were used. For the first time, the values of ELISA are objectively displayed in the current study. Sex evaluations were performed on each sample using the newly designed In-House indirect ELISA. Three are related to the cutoff value, two are related to the sample's initial dilution, and the last reading is related to the antibody index. The values of the Majaheem breed with follicular and luteal cyst were significantly greater than those of healthy she-camels in terms of serum OD of primary dilution; cut-off (antibody titer) value and calibrated antibody concentration against ovarian protein (P<0.05 and P<0.01, respectively). Concerning antibody index, the data demonstrated a significant difference between the healthy she-camel and Majaheem breed with follicular and luteal cyst (P<0.05), Majaheem breed with hemorrhagic cyst (P<0.01) and Majaheem and Waddah breeds with inactive ovary (P<0.05). Results exhibited a significant decrease in estrogen (P<0.001) and FSH levels (P<0.05) comparing to normal control group in the serum of Majaheem breed with follicular and luteal cysts and inactive ovary. It is recommended that more study be done on the blood-follicle barrier to comprehend how abnormality develops in ovarian she-dromedaries.