The involvement of 1-aminocyclopropane-1-carboxylic acid synthase isogene, Pp-ACS1, in peach fruit softening

Tatsuki, Miho; Haji, Takashi; Yamaguchi, Masami

doi:10.1093/jxb/erj097

Cited by 86 publications

(60 citation statements)

References 29 publications

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“…This ethylene increase did not correlate with PpACO1 content (Figure 8C). It is known that the main regulatory step in the ethylene synthesis in peaches is related to the Prunus persica 1-aminocyclopropane-1-carboxylate synthase 1 (PpACS1) transcriptional and/or translational control [64]. However, we did not identify PpACS1 in this study and therefore cannot rule out the possibility that the ethylene increase observed in the E4 stage is associated to this protein.…”

Section: Discussionmentioning

confidence: 73%

Proteomic analysis of peach fruit mesocarp softening and chilling injury using difference gel electrophoresis (DIGE)

et al. 2010

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BackgroundPeach fruit undergoes a rapid softening process that involves a number of metabolic changes. Storing fruit at low temperatures has been widely used to extend its postharvest life. However, this leads to undesired changes, such as mealiness and browning, which affect the quality of the fruit. In this study, a 2-D DIGE approach was designed to screen for differentially accumulated proteins in peach fruit during normal softening as well as under conditions that led to fruit chilling injury.ResultsThe analysis allowed us to identify 43 spots -representing about 18% of the total number analyzed- that show statistically significant changes. Thirty-nine of the proteins could be identified by mass spectrometry. Some of the proteins that changed during postharvest had been related to peach fruit ripening and cold stress in the past. However, we identified other proteins that had not been linked to these processes. A graphical display of the relationship between the differentially accumulated proteins was obtained using pairwise average-linkage cluster analysis and principal component analysis. Proteins such as endopolygalacturonase, catalase, NADP-dependent isocitrate dehydrogenase, pectin methylesterase and dehydrins were found to be very important for distinguishing between healthy and chill injured fruit. A categorization of the differentially accumulated proteins was performed using Gene Ontology annotation. The results showed that the 'response to stress', 'cellular homeostasis', 'metabolism of carbohydrates' and 'amino acid metabolism' biological processes were affected the most during the postharvest.ConclusionsUsing a comparative proteomic approach with 2-D DIGE allowed us to identify proteins that showed stage-specific changes in their accumulation pattern. Several proteins that are related to response to stress, cellular homeostasis, cellular component organization and carbohydrate metabolism were detected as being differentially accumulated. Finally, a significant proportion of the proteins identified had not been associated with softening, cold storage or chilling injury-altered fruit before; thus, comparative proteomics has proven to be a valuable tool for understanding fruit softening and postharvest.

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Section: Discussionmentioning

confidence: 73%

Proteomic analysis of peach fruit mesocarp softening and chilling injury using difference gel electrophoresis (DIGE)

et al. 2010

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“…Several of these differentially expressed genes are likely involved in terpene and gibberellin synthesis, ethylene production and signaling, cell-wall remodeling, cell division and defense responses (Additional file 1). In particular, we noticed that the ethylene synthesis pathway represented by orthologs of ACC synthase, ACC oxidase and AP2 factor genes, which play roles in cell wall softening [32-34], appears to be a primary mechanism elicited in the host after Xc sensing. On the other hand, defense response genes encoding chitinases, WRKY factors and pathogenesis-related (PR) proteins are also rapidly induced (Additional file 1).…”

Section: Resultsmentioning

confidence: 99%

Identification of putative TAL effector targets of the citrus canker pathogens shows functional convergence underlying disease development and defense response

et al. 2014

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BackgroundTranscriptional activator-like (TAL) effectors, formerly known as the AvrBs3/PthA protein family, are DNA-binding effectors broadly found in Xanthomonas spp. that transactivate host genes upon injection via the bacterial type three-secretion system. Biologically relevant targets of TAL effectors, i.e. host genes whose induction is vital to establish a compatible interaction, have been reported for xanthomonads that colonize rice and pepper; however, citrus genes modulated by the TAL effectors PthA“s” and PthC“s” of the citrus canker bacteria Xanthomonas citri (Xc) and Xanthomonas aurantifolii pathotype C (XaC), respectively, are poorly characterized. Of particular interest, XaC causes canker disease in its host lemon (Citrus aurantifolia), but triggers a defense response in sweet orange.ResultsBased on, 1) the TAL effector-DNA binding code, 2) gene expression data of Xc and XaC-infiltrated sweet orange leaves, and 3) citrus hypocotyls transformed with PthA2, PthA4 or PthC1, we have identified a collection of Citrus sinensis genes potentially targeted by Xc and XaC TAL effectors. Our results suggest that similar with other strains of Xanthomonas TAL effectors, PthA2 and PthA4, and PthC1 to some extent, functionally converge. In particular, towards induction of genes involved in the auxin and gibberellin synthesis and response, cell division, and defense response. We also present evidence indicating that the TAL effectors act as transcriptional repressors and that the best scoring predicted DNA targets of PthA“s” and PthC“s” in citrus promoters predominantly overlap with or localize near to TATA boxes of core promoters, supporting the idea that TAL effectors interact with the host basal transcriptional machinery to recruit the RNA pol II and start transcription.ConclusionsThe identification of PthA“s” and PthC“s” targets, such as the LOB (LATERAL ORGAN BOUNDARY) and CCNBS genes that we report here, is key for the understanding of the canker symptoms development during host susceptibility, or the defenses of sweet orange against the canker bacteria. We have narrowed down candidate targets to a few, which pointed out the host metabolic pathways explored by the pathogens.

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“…These results indicate that in the stony hard peach, ACC oxidase activity and ethylene sensing are normal, and endogenous ethylene production is inhibited due to the inability to synthesize ACC. To clarify the suppression mechanism of ethylene production in the stony hard peaches, Tatsuki et al examined the expression levels of ACC synthases (Pp-ACS1, Pp-ACS2, and Pp-ACS3) and ACC oxidase (Pp-ACO1) in melting and stony hard cultivars 18 . They concluded that inhibition of endogenous ethylene production in the stony hard peach fruit was due to the suppressed expression of Pp-ACS1 mRNA.…”

Section: Disciplinementioning

confidence: 99%

The involvement of 1-aminocyclopropane-1-carboxylic acid synthase isogene, Pp-ACS1, in peach fruit softening

Cited by 86 publications

References 29 publications

Proteomic analysis of peach fruit mesocarp softening and chilling injury using difference gel electrophoresis (DIGE)

Proteomic analysis of peach fruit mesocarp softening and chilling injury using difference gel electrophoresis (DIGE)

Identification of putative TAL effector targets of the citrus canker pathogens shows functional convergence underlying disease development and defense response

Inheritance of Flesh Texture in Peach and Effects of Ethylene Treatment on Softening of the Stony Hard Peach

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