The invasion biology of tomato begomoviruses in Costa Rica reveals neutral synergism that may lead to increased disease pressure and economic loss

Maliano, Minor R.; Rojas, María R.; Macedo, M. A.; Barboza, Natalia; Gilbertson, R. L.

doi:10.1016/j.virusres.2022.198793

Cited by 9 publications

(7 citation statements)

References 112 publications

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“…This research shows how the TYLCV infection in tomato plants in Costa Rica has spread throughout most of the country and has masked infections with other begomoviruses (PepGMV, ToYMoV and ToLCSiV) as also shown in Maliano et al (2022) research. A slight increase in the number of mixed infections was reflected between 2015 and 2016.…”

Section: Discussionsupporting

confidence: 72%

Begomovirus diversity in tomato crops in Costa Rica

Valverde‐Méndez,

Hernández,

Matamoros

et al. 2023

Annals of Applied Biology

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Begomoviruses (Geminiviridae family) are characterized by their high recombination rate and a wide range of hosts, making their control difficult. In Costa Rica, various species of bipartite begomoviruses have been reported, which are Pepper golden mosaic virus (PepGMV), Tomato yellow mottle virus (ToYMoV), Tomato leaf curl Sinaloa virus (ToLCSiV) and the monopartite begomovirus Tomato yellow leaf curl virus (TYLCV). Since the TYLCV first report in Costa Rica, neither additional knowledge has been produced on how this begomovirus has spread in the country's territory nor on the distribution of the other bipartite species. A total of 429 tomato samples collected during the years 2015–2016 were used to study these aspects. Each sample was georeferenced and analysed with various techniques such as nucleic acid hybridization, polymerase chain reaction (PCR) and sequencing for the begomoviruses previously reported in Costa Rica. It was found that the presence/absence of the different species can vary, depending on the province. TYLCV is present in the six provinces analysed in this work, with a proportion from 3.7 to 86.6 per cent. Alajuela, Cartago, and Heredia are the provinces most affected by tomato‐infecting begomoviruses. Fourteen different haplotypes of TYLCV were detected, but all were identified as TYLCV‐IL. The distribution of TYLCV was related to the presence of the whitefly Bemisia tabaci MED, especially in the country's main tomato production areas. This information allows the phytosanitary surveillance services to develop strategies for the integrated management of the disease and to contribute data to the genetic improvement programmes of the crop.

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Section: Discussionsupporting

confidence: 72%

Begomovirus diversity in tomato crops in Costa Rica

Valverde‐Méndez,

Hernández,

Matamoros

et al. 2023

Annals of Applied Biology

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“…Thus, movement is potentially a highly evolvable redundant function of multiple genes that may support inter-cellular movement for the no-V2 monopartite begomoviruses. Alternatively, it was proposed that phloem-limited begomoviruses may have a reduced need for protein- mediated inter-cellular movement [27, 128], although this needs experimental assessment. The phloem-limited monopartite Americas begomoviruses ToLDeV [60] and ToMoLCV [27] provide an excellent system to explore this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

Phylogeographic analysis ofBegomoviruscoat and replication-associated proteins

Crespo-Bellido,

Hoyer,

Burgos-Amengual

et al. 2023

Preprint

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Begomoviruses are globally distributed plant pathogens that significantly limit crop production. These viruses are traditionally described according to phylogeographic distribution and categorized into two groups: begomoviruses from the Africa, Asia, Europe, and Oceania (AAEO) region and begomoviruses from the Americas. Monopartite begomoviruses are more common in the AAEO region while bipartite viruses predominate in the Americas, where the begomoviruses lack the V2/AV2 gene involved in inter-cellular movement and RNA silencing suppression found in AAEO begomoviruses. While these features are generally accepted as lineage-defining, the number of known species has doubled due to sequence-based discovery since 2010. To reevaluate the geographic groupings after the rapid expansion of the genus, we conducted phylogenetic analyses for begomovirus species representatives of the two longest and most conserved begomovirus proteins: the coat and replication-associated proteins. Both proteins still largely support the broad AAEO and Americas begomovirus groupings, except for sweetpotato-infecting begomoviruses that form an independent, well-supported clade for their coat protein regardless of the region they were isolated from. Our analyses do not support more fine-scaled phylogeographic groupings. Monopartite and bipartite genome organizations are broadly interchanged throughout the phylogenies and the absence of the V2/AV2 gene is highly reflective of the split between Americas and AAEO begomoviruses. We observe significant evidence of recombination within the Americas and within the AAEO region, but rarely between the regions. We speculate that increased globalization of agricultural trade, the invasion of polyphagous whitefly vector biotypes and recombination will blur begomovirus phylogeographic delineations in the future.

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“…The interaction between monopartite begomoviruses and their cognate or non-cognate betasatellites promotes virus infection. In mixed infections, many begomoviruses co-existing in natural host plants have shown neutral synergistic interactions [ 50 , 51 , 52 ], which may lead to more severe disease complexes. The finding that tomato and muskmelon plants harbor a mixed infection of CLCuGeV and its associated DNA-satellites together with TYLCV or ToLCPalV might suggest that these viruses may complement each other, as is the case of previous reports on TYLCV [ 50 , 53 , 54 ].…”

Section: Discussionmentioning

confidence: 99%

“…In mixed infections, many begomoviruses co-existing in natural host plants have shown neutral synergistic interactions [ 50 , 51 , 52 ], which may lead to more severe disease complexes. The finding that tomato and muskmelon plants harbor a mixed infection of CLCuGeV and its associated DNA-satellites together with TYLCV or ToLCPalV might suggest that these viruses may complement each other, as is the case of previous reports on TYLCV [ 50 , 53 , 54 ]. Nevertheless, the association of OLCuOMB and OYCrCMA might be a host-switching strategy because CLCuGeV is mostly associated with CLCuGeB and/or CLCuGeA [ 19 , 34 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

High-Throughput Sequencing Identified Distinct Bipartite and Monopartite Begomovirus Variants Associated with DNA-Satellites from Tomato and Muskmelon Plants in Saudi Arabia

Alhudaib

Almaghasla

El-Ganainy

et al. 2022

Plants

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The studies on the prevalence and genetic diversity of begomoviruses in Saudi Arabia are minimal. In this study, field-grown symptomatic tomato and muskmelon plants were collected, and initially, begomovirus infection was confirmed by the core coat protein sequences. Four tomato and two muskmelon plants with viral infections were further evaluated for Illumina MiSeq sequencing, and twelve sequences (2.7–2.8 kb) equivalent to the full-length DNA-A or DNA-B components of begomoviruses were obtained along with eight sequences (~1.3–1.4 kb) equivalent to the begomovirus-associated DNA-satellite components. Four begomovirus sequences obtained from tomato plants were variants of tomato yellow leaf curl virus (TYLCV) with nt sequence identities of 95.3–100%. Additionally, two tomato plants showed a mixed infection of TYLCV and cotton leaf curl Gezira virus (CLCuGeV), okra yellow crinkle Cameroon alphasatellite (OYCrCMA), and okra leaf curl Oman betasatellite (OLCuOMB). Meanwhile, from muskmelon plants, two sequences were closely related (99–99.6%) to the tomato leaf curl Palampur virus (ToLCPalV) DNA-A, whereas two other sequences showed 97.9-100% sequence identities to DNA-B of ToLCPalV, respectively. Complete genome sequences of CLCuGeV and associated DNA-satellites were also obtained from these muskmelon plants. The nt sequence identities of the CLCuGeV, OYCrCMA, and OLCuOMB isolates obtained were 98.3–100%, 99.5–100%, and 95.6–99.7% with their respective available variants. The recombination was only detected in TYLCV and OLCuOMB isolates. To our knowledge, this is the first identification of a mixed infection of bipartite and monopartite begomoviruses associated with DNA-satellites from tomato and muskmelon in Saudi Arabia. The begomovirus variants reported in this study were clustered with Iranian isolates of respective begomovirus components in the phylogenetic dendrogram. Thus, the Iranian agroecological route can be a possible introduction of these begomoviruses and/or their associated DNA-satellites into Saudi Arabia.

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The invasion biology of tomato begomoviruses in Costa Rica reveals neutral synergism that may lead to increased disease pressure and economic loss

Cited by 9 publications

References 112 publications

Begomovirus diversity in tomato crops in Costa Rica

Begomovirus diversity in tomato crops in Costa Rica

Phylogeographic analysis ofBegomoviruscoat and replication-associated proteins

High-Throughput Sequencing Identified Distinct Bipartite and Monopartite Begomovirus Variants Associated with DNA-Satellites from Tomato and Muskmelon Plants in Saudi Arabia

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