2024
DOI: 10.1128/aac.00452-24
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The intrinsic macrolide resistome of Escherichia coli

Yibing Ma,
Mattia Pirolo,
Bimal Jana
et al.

Abstract: Intrinsic resistance to macrolides in Gram-negative bacteria is primarily attributed to the low permeability of the outer membrane, though the underlying genetic and molecular mechanisms remain to be fully elucidated. Here, we used transposon directed insertion-site sequencing (TraDIS) to identify chromosomal non-essential genes involved in Escherichia coli intrinsic resistance to a macrolide antibiotic, tilmicosin. We constructed two highly saturated transposon mutant libraries of … Show more

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