The intriguing interaction of Dicer (DCR-1) with GLH-1, a P granule component in Caenorhabditis elegans

Racen, Erica L.; McEwen, Tamara J.; Bennett, Karen

doi:10.1016/j.ydbio.2008.05.288

Cited by 3 publications

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“…The miRNA pathway-dedicated complex involves interaction between Dicer and ALG-1/2 and, eventually, LIN-41 regulating let-7 miRNA (Duchaine et al, 2006). The list of Dicer-binding proteins in C. elegans is even longer and involves proteins with roles in different aspects of silencing or with yet undetermined functions (Beshore et al, 2009;Beshore et al, 2011;Duchaine et al, 2006;Racen et al, 2008).…”

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confidence: 99%

Introduction to RNAi and miRNA pathways

Svoboda¹

2020

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edge hereby that the contents have been produced under a contract with EFSA (OC/EFSA/ GMO/2015/01-CT 01) and that the opinions expressed are those of the contractor only and do not represent EFSA's official position.Regarding the conversion, the original text was reorganized into twelve chapters, which were reformatted and revised in order to remove copyrighted material from third parties and provide a introductory parts for stand alone chapters. Nine of the chapters focus on small RNA pathways in animals (mammals, birds, fish, arthropods, nematodes, molluscs, and annelids) and plants. The remaining three chapters include a general introduction and reviews of important phenomena -off-targeting and extracellular small RNAs. I hope that this collection will serve as a useful source for many.

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Section: )mentioning

confidence: 99%

Introduction to RNAi and miRNA pathways

Svoboda¹

2020

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Section: Dicermentioning

confidence: 99%

RNAi and miRNA pathways in nematodes

Svoboda¹

2020

Introduction to RNAi and miRNA Pathways

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RNA silencing denotes sequence-specific repression mediated by small RNAs. In metazoa, there are two mechanistically closely related pathways: RNA interference (RNAi) and microRNA (miRNA) pathway. The miRNA pathway regulates endogenous protein-coding gene expression and has been implicated in many biological processes. RNAi generally serves as a form of innate immunity targeting viruses and mobile elements. This review is summarizing information about RNAi and miRNA pathways in the phylum Nematoda. Research on miRNA and RNAi pathways in the phylum Nematoda has been primarily using Caenorhabditis elegans model system and related species. In fact, miRNA and RNAi pathways were both discovered in this model system. Studies in Caenorhabditis elegans revealed highly complex organization of small RNA pathways despite there is just a single Dicer gene. The complexity of small RNA pathways in Caenorhabditis elegans stems from a large number of Argonaute proteins and three RNA-dependent RNA polymerases, which establish an intricate system of primary and secondary small RNAs with different biological functions in the cytoplasm and nucleus. This review focuses on the miRNA pathway and pathways initiated by long dsRNA. The first part introduces the key molecular players of RNA silencing in nematodes. The second summarizes miRNA and dsRNA-induced mechanisms, in this case RNAi (exogenous = exo-RNAi and endogenous = endo-RNAi) and adenosine deamination (nematodes do not have the interferon system like vertebrate groups).

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Literature review of baseline information to support the risk assessment of RNAi‐based GM plants

Pačes

Nič²,

Novotný³

et al. 2017

EFS3

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This report is the outcome of an EFSA procurement aiming at investigating and summarising the state of knowledge on (I) the mode-of-action of dsRNA and miRNA pathways, (II) the potential for nontarget gene regulation by dsRNA-derived siRNAs or miRNAs, (III) the determination of siRNA pools in plant tissues and the importance of individual siRNAs for silencing. The report is based on a comprehensive and systematic literature search, starting with the identification and retrieval of~190,000 publications related to the research area and further filtered down with keywords to produce focused collections used for subsequent screening of titles and abstracts. The report is comprised of an (I) Introduction to the field of small RNAs, (II) a Data and Methodologies section containing strategies used for literature search and study selection, and (III) the Results of the literature review organized according to the three main procurement tasks. The outcome of the first task reviews dsRNA and miRNA pathways in mammals (including humans), birds, fish, arthropods, annelids, molluscs, nematodes, and plants. Eight taxon-dedicated chapters are based on~1,400 cumulative references chosen from~10,000 inspected titles and abstracts. We review conserved and divergent aspects of small RNA pathways and dsRNA responses in animals and plants including structure and function of key proteins as well as four basic mechanisms: genome-encoded posttranscriptional regulations (miRNA), degradation of RNAs by short interfering RNA pools generated from long dsRNA (RNAi), transcriptional silencing, and sequence-independent responses to dsRNA. The outcome of the second task focuses on base pairing between small RNAs and their target RNAs and predictability of biological effects of small RNAs in animals and plants. The outcome of the last task reviews methodology, siRNA pools, and movement of small RNAs in plants. Potential transfer of small RNAs between species and circulating miRNAs in mammals is described in the final chapter.

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The intriguing interaction of Dicer (DCR-1) with GLH-1, a P granule component in Caenorhabditis elegans

Cited by 3 publications

References 0 publications

Introduction to RNAi and miRNA pathways

Introduction to RNAi and miRNA pathways

RNAi and miRNA pathways in nematodes

Literature review of baseline information to support the risk assessment of RNAi‐based GM plants

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