The intragenic mRNA-microRNA regulatory network during telogen-anagen hair follicle transition in the cashmere goat

Liu, Zhihong; Yang, Feng; Zhao, Meng; Li, Haijun; Xie, Yingxin; Nai, Rile; Che, Tianyu; Su, Rui; Zhang, Yanjun; Wang, Ruijun; Wang, Zhiying; Li, Jinquan

doi:10.1038/s41598-018-31986-2

Cited by 15 publications

(15 citation statements)

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“…The transitions between phases in the dynamic hair cycle are influenced by interactions between a series of growth signals and inhibitory molecules controlling the growth of hair follicles. Recently, the intragenic mRNA-miRNA regulatory network during the telogen-anagen transition was investigated, demonstrating that hair follicle growth initiation and development were related to miR-195 and the genes CHP1, SMAD2, FZD6, and SIAH1 in the cashmere goat 5 . Here, to elucidate the molecular mechanisms regulating hair follicle cycling, miRNA expression profiles were investigated in the skin tissue of Wan Strain Angora rabbits, after reconstructing hair follicle cycling.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the DE miRNAs were potential regulators of hair follicle development and lipid metabolism in rabbits. In addition, miRNAs may regulate the hair cycle through key genes controlling signalling pathways that modulate hair follicle growth 5,40 . The MAPK signalling pathway, Wnt signalling pathway, ECM-receptor interaction, as well as fat and fatty acid metabolism pathways, were enriched by target genes of DE miRNAs.…”

Section: Discussionmentioning

confidence: 99%

“…Small RNA library construction, sequencing and analysis. The whole skin including the dermis and dermal cells was used for sequencing 5,40,43 , and hair follicles were the parts of the dermis. Skin samples were isolated from the dorsal skin of three Wan Strain Angora rabbits in the first week after hair plucking (S01 samples), when the wool cycle is expected to be in the telogen phase, and in the eighth week post-plucking (S02 samples), i.e., in the anagen phase.…”

Section: Methodsmentioning

confidence: 99%

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Analysis of histological and microRNA profiles changes in rabbit skin development

Ding

Cheng

Leng

et al. 2020

Sci Rep

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The periodic regrowth of rabbit fur is economically important. Here, we aimed to characterise the histological traits and microRNA (miRNA) expression profiles in the skin tissue of Wan Strain Angora rabbits at different weeks after plucking. Haematoxylin-eosin staining showed that hair follicles were in the telogen phase in the first week, while they were in the anagen phase from the fourth to twentyfourth weeks. In addition, two small RNA libraries derived from skin samples of Wan Strain Angora rabbits at telogen and anagen stages yielded over 24 million high-quality reads. Specifically, 185 miRNAs were differentially expressed between the telogen and anagen phases. The function of the differentially expressed miRNAs was explored by comparing them with known mammalian miRNAs and by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis of their predicted targets. Five new functional miRNAs were validated using quantitative real-time PCR. Moreover, the fibroblast growth factor 5 (FGF5) gene was verified to be a target of conservative_NC_013672.1_9290 and conservative_NC_013675.1_10734. We investigated differential miRNA profiles between the telogen and anagen phases of the hair cycle and our findings provide a basis for future studies focusing on the mechanisms of miRNA-mediated regulation of rabbit hair follicle cycling.The growth and development of hair follicles are cyclical throughout a rabbit's life. In adults, hair follicles undergo cyclical bouts of growth (anagen), destruction (catagen), and rest (telogen), which are collectively known as the hair cycle 1-4 . Each growth period has a specific activated/silenced gene expression pattern 5 . Hence, understanding gene expression patterns can help to elucidate the regulatory mechanisms of the rabbit hair cycle.Hair follicle development is controlled at several levels, including epigenetic regulation and transcription factor-induced signalling 6,7 . miRNAs are small (22 nucleotides) noncoding regulatory RNAs that reduce stability and/or the translation of specific mRNA targets with full or partial complementary sequences 8-10 . However, compared with other mammals, including humans (Homo sapiens), mice (Mus musculus), and rats (Rattus norvegicus), relatively few miRNAs in rabbits have been verified and deposited in the public miRBase database. Thus, it is necessary to clarify the roles of miRNAs in the regulation of the hair cycle in rabbits.Emerging evidence indicates that miRNAs are involved in skin and hair follicle development. miRNA/mRNA regulatory networks are reported to be involved in the regulation of hair follicle development and epidermal homeostasis 11 . In particular, hair follicle development is regulated by miR-195-5p-induced inhibition of the Wnt/β-catenin pathway through targeting LRP6 and signature genes of Wnt signalling 12 . Furthermore, miR-339-5p negatively regulates loureirin A-induced hair follicle stem cell differentiation by targeting DLX5 13 . In addition, miR-31 is a marker of the hair growth phase, and its loss p...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Analysis of histological and microRNA profiles changes in rabbit skin development

Ding

Cheng

Leng

et al. 2020

Sci Rep

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“…Concurrent with HFs' yearly sequential switching from growth stage (anagen), regression stage (catagen), and resting stage (telogen), the cashmere annually elongates and sheds [2,3]. In early efforts to delineate molecular mechanisms governing the dynamic transitions of HFs among these stages and seasonal cashmere regrowth, a large quantity of protein-coding and non-protein-coding transcripts [4,5], as well as several epigenetic modifications on genomic and post-transcriptional levels [6,7], have been filtered as potential participators via utilizing high-throughput-based sequencing strategies, such as RNA-seq. For instance, we previously performed a comprehensive transcript profiling of goat skin tissues at anagen and catagen, then simultaneously identified a total of 3500 mRNAs, 72 microRNAs (miRNAs), and 173 long noncoding RNAs (lncRNAs) that are differentially expressed between the two stages, hinting that the anagen-to-catagen transition may be under the guidance of an intricate network constituted by numerous lines of interacting coding and noncoding genes [5].…”

Section: Introductionmentioning

confidence: 99%

Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified Applications

Wang

Zong

et al. 2020

Animals

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A functional interpretation of filtered candidates and predicted regulatory pathways related to cashmere growth from sequencing trials needs available cell models, especially for hair matrix cells (HMCs), whose continual proliferation and differentiation result in rapid hair growth. To fulfill such goals, we herein obtained primary goat HMCs via a microdissection-based method; optimized the selection of the culture medium and coating substances for better cell maintenance; and exemplified their usefulness through examining the effects of calcium and all-trans retinoic acid (ATRA) on cells using immunoblotting, flow cytometry, and other techniques. As a result, we successfully acquired primary and passaged goat HMCs with typical keratinocyte morphology. Calcium-free RPMI (Roswell Park Memorial Institute) 1640 and MEM (minimum Eagle’s medium) outperformed normal DMEM/F12 (Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12) on long-term cell maintenance, whereas serum-free media K-SFM and EpiLife failed to support cell growth. HMCs differed molecularly and morphologically from their neighbor dermal papilla cells on expressions of feature genes, such as HOXC13, and on characteristic keratinocyte-like appearances versus fibroblast shapes, respectively. Higher calcium concentrations significantly stimulated the expression of the genes (e.g., KRT1 and IVL) involved in keratinocyte differentiation and, promoted cell proliferation. Moreover, 10−5 M ATRA obviously boosted goat HMC expansions and changed their cell cycle distributions compared to the controls. Our study shines a light on researches exploring the mechanisms underlying the growth of cashmere.

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“…Previous studies have shown that the number of DP cells determines the number of hair follicles and the density of secondary hairs, and have revealed the heterogeneity of DP cells in different types of hair follicles [17,18]. The hair of cashmere goats is divided into primary hair (guard hair) and secondary hair (ground hair or cashmere) [19,20]. The cashmere quality of cashmere goats has a signi cant relationship with the secondary hair follicles [21], but the speci c regulatory mechanism has not been fully explained.…”

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confidence: 99%

Single-cell sequencing reveals the intermediate cell state and function of dermal papilla cells in the hair follicle cycle of cashmere goats

Yang¹,

Liu²,

Che³

et al. 2020

Preprint

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Background: Human hair loss and regeneration has stimulated interest in the natural hair cycle worldwide; however, such research is difficult because the periodicity of human or mouse hair is not visually obvious. Dermal papilla cells (DP cells) play an important role in the development of hair follicles, but knowledge of the differentiation and mechanisms of DP stem cells during transition through the hair follicle cycle are still limited, although some studies have reported that DP cells may have an intermediate cell state during differentiation, the classification and function of specific cell states are not clear. Results: Here, we used cashmere goats, that have obvious periodicity of hair follicles, as model animals and, based on unbiased single cell RNA sequencing, we identified and isolated DP cell data. Pseudotime ordering analysis was used to successfully construct a DP cell lineage differentiation trajectory and revealed the sequential activation of key genes, signaling pathways, and functions involved in cell fate decisions. At the same time, we analyzed the mechanisms of different cell fates and revealed the function of four different intermediate cells: Intermediate cells 10 showed important functions in the growth of cashmere and maintenance of cashmere attachment to the skin; intermediate cells 1 revealed important functions in the process of apoptosis and cashmere shedding of secondary hair follicles; intermediate cells 0 initiated new follicular cycles and completed the migration of hair follicles and the occurrence of cashmere; and intermediate cells 15 are suggested to be DP progenitor cells. Conclusions: In development and apoptosis, inner bulge cells not only earlier than outer bulge cells, but occurred faster and was more thorough,this helps a deeper understanding of the role of bulge cells. Pseudogenes play another important role in function which promoted the competitive endogenous RNA (ceRNA) hybridization of pseudogenes.In different hair follicle cycles, DP cells will differentiate into different intermediate state cells and perform different functions, and the marker genes of the cells also changed. Intermediate cells 10 showed important functions in the growth of cashmere and maintenance of cashmere attachment to the skin; intermediate cells 1 revealed important functions in the process of apoptosis and cashmere shedding of secondary hair follicles; intermediate cells 0 initiated new follicular cycles and completed the migration of hair follicles and the occurrence of cashmere; and intermediate cells 15 are DP progenitor cells, this conclusion provides an unprecedented deeper understanding of the function of DP cells.

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The intragenic mRNA-microRNA regulatory network during telogen-anagen hair follicle transition in the cashmere goat

Cited by 15 publications

References 50 publications

Analysis of histological and microRNA profiles changes in rabbit skin development

Analysis of histological and microRNA profiles changes in rabbit skin development

Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified Applications

Single-cell sequencing reveals the intermediate cell state and function of dermal papilla cells in the hair follicle cycle of cashmere goats

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