The Intracisternal A-Particle Gene Family: Structure and Functional Aspects

Kuff, Edward L.; Lueders, Kira K.

doi:10.1016/s0065-230x(08)60223-7

Cited by 326 publications

(312 citation statements)

References 243 publications

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“…14,24,26 Plasmacytoma IAP-LTRs contain a typical ATF sequence, while the normal lymphocytes IAP-LTRs conDiscussion tain an altered ATF sequence. 14,24,26 The LTRs present in LSIAPs were relatively weak in stimulating sufficient IL-3 In the present study, we have investigated the effects of certain LTRs on activating the transcription of a germline IL-3 gene.…”

Section: Figurementioning

confidence: 99%

“…LS-IAPs are normally expressed in lipopolysaccharide-stimulated normal B cells, while PC-IAPs are expressed Factor-independent cells were very infrequently recovered after transfection of IL-3-dependent cells with the gIL3 conin tumor cells. 14,26 Transpositions of PC-IAPs normally affect the expression of the adjacent genes more profoundly than taining the HIV, and HTLV LTRs. These results indicated that these human retroviral cis-acting elements are not sufficient LS-IAPs.…”

Section: Figurementioning

confidence: 99%

“…Organs and cells which express low levels of IAPs Acute oncogenic retroviruses, which contain an oncogene, include: spleen, muscle, pancreas, ovary, thymus, placenta, cause cancer after a short period of time post-infection of a macrophage and fibroblast cells. 14,27 IAP expression is under susceptible host, ranging from one to a few months. Chronic genetic control in non-neoplastic cells.…”

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confidence: 99%

“…Mechanisms Moreover, there are sequence differences between the LTR which result in abnormal gene expression after proviral intecDNAs isolated from the plasmacytoma cells and normal lymphocytes. 14,26 These observations gave rise to the nomenclature plasmacytoma (PC) IAP and lymphocyte specific (LS)…”

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Differential effects of retroviral long terminal repeats on interleukin-3 gene expression and autocrine transformation

McCubrey

1997

Leukemia

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Section: Figurementioning

confidence: 99%

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confidence: 99%

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Differential effects of retroviral long terminal repeats on interleukin-3 gene expression and autocrine transformation

McCubrey

1997

Leukemia

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“…The intracisternal A-particle (IAP), believed to be a defective provirus, is the most studied retrotransposon in the mouse (see reviews in [1,2]). Like a retrovirus, the integrated form of IAP in the mouse genome has gag-pol-env genes between two direct repeats of a retroviral long terminal repeat (LTR) sequence.…”

Section: Introductionmentioning

confidence: 99%

Unusual long target duplication by insertion of intracisternal A‐particle element in radiation‐induced acute myeloid leukemia cells in mouse

Tanaka¹,

Ishiguro²

1995

FEBS Letters

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Retrotransmission into the IL-31GM-CSF gene locus by the retrotransposon intracisternal A-particle (IAP) had been observed in distinct tumor cell lines. We analyzed the locus in genomes from 7 different myeloid leukemia cell strains which were originally generated by whole-body X-irradiation of the inbred C3H/He mice at a dose of 3 Gy and maintained by in vivo passage. In one leukemia cell strain out of 7 such cases, RFLP of an allele of the interleukin-3 gene was found. Sequence analysis after cloning from the genomic library showed that a type IA2 IAP element was inserted in the region upstream of the IL-3 gene in the head-to-head orientation. This suggests that the locus in myeloid cells is sensitive for integration of IAP elements. Additionally, an unusual long target duplication of 82 bp, 14-fold larger than normal one, was found at the junction of the element. This suggests the possibility of a radiation-induced integration mechanism which is distinct from normal retrotransmission. Key words:Retrotransposon; Intracisternal A-particle; Myeloid leukemia; Target duplication; Interleukin-3 gene; Genomic DNA cloning with gene rearrangements of IL-3 by retrotransmission should be detected. To confirm this supposition as well as to study the chronic effects of radiation, we used radiation-induced acute myeloid leukemia cells from the C3H/He mouse for analysis. Approximately 20-30% of whole-body X-irradiated C3H/He mice develop acute myeloid leukemia within 2 years with chromosomal aberrations [9,10]. Each leukemia cell strain from different individuals should be an 'independent' leukemia since they became tumorigenic by separate processes. In leukemia cells, not only tumor-specific but also radiation-specific events may be recorded in the genome.We analyzed this locus and found integration of the IAP element in the 1L-3 gene in one leukemia cell strain from a total of 7 independent leukemias. Additionally, an unusual long direct repeat of mouse DNA of 82 bp in length was generated at the insertion site of the lAP element, although the target duplication is no more than 6 bp in length in all the reported sequences suggesting a retroviral-like integration mechanism [3,5,6,8,[11][12][13][14][15][16][17][18][19]. The long target duplication may reflect a specific event which occurs during radiation-induced leukemogenesis.

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