The intracellular drug delivery and anti tumor activity of doxorubicin loaded poly(γ-benzyl l-glutamate)-b-hyaluronan polymersomes

Upadhyay, Kamal K.; Bhatt, Anant Narayan; Mishra, Anil K.; Dwarakanath, Bilikere S.; Jain, Sanyog; Schatz, Christophe; Meins, Jean-François Le; Farooque, Abdullah; Godugu, Chandraiah; Jain, Amit; Misra, Ambikanandan; Lecommandoux, Sébastien

doi:10.1016/j.biomaterials.2009.12.043

Cited by 306 publications

(237 citation statements)

References 44 publications

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“…The mechanism underlying the controlled slow release of hormone/drug in HA has been examined in several studies (Kim and Park 2002;Lehr and Haas 2002;Cho et al 2003;Lee et al 2010;Oh et al 2010;Upadhyay et al 2010). The basis of the interaction between HA and the deliverable agent was a multivalent anionic charge, resulting in the diffusion of hormones in each layer of the HA polymer surface.…”

Section: Discussionmentioning

confidence: 99%

A simplified superovulation protocol using split-single administration of Folltropin®-V in hyaluronan: application to purebred sheep

Panyaboriban¹,

Suwimonteerabutr²,

Swangchan-Uthai³

et al. 2018

Vet. Med. - Czech

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ABSTRACT:Superovulation is an important step in assisted reproductive technology. Due to its short half-life, follicle stimulating hormone is usually given twice daily to ewes for three to five days, which is both time-and labour-intensive. However, dissolving follicle stimulating hormone in degradable polymers to delay absorbtion has been effective in ruminants. Experiment 1 was performed to compare a split-single follicle stimulating hormone dissolved in hyaluronan (S group; 150 mg follicle stimulating hormone on the first day and 30 mg 48 h later; n = 21) and six decreasing doses of follicle stimulating hormone (M group; 50, 50, 30, 30, 10 and 10 mg; n = 22) at 12-h intervals. Ovarian responses and numbers of recovered ova/embryos did not differ significantly between groups. However, there tended to be more Grade 1 and 2 embryos in S vs M groups (mean ± SEM, 5.1 ± 4.9 vs 2.9 ± 2.9, respectively; P = 0.08). Experiment 2 tested the effectiveness of a simplified split-single follicle stimulating hormone in purebred sheep on a commercial farm. The numbers of recovered good-grade embryos (day 2) were 4.8 ± 5.0 and 4.0 ± 2.5 per donors in Corriedale and Bond sheep breeds, respectively. We conclude that this modified technique for ewe superovulation improved animal welfare, reduced animal handling and labour and yielded results similar to or better than conventional twice-daily follicle stimulating hormone treatments.

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Section: Discussionmentioning

confidence: 99%

A simplified superovulation protocol using split-single administration of Folltropin®-V in hyaluronan: application to purebred sheep

Panyaboriban¹,

Suwimonteerabutr²,

Swangchan-Uthai³

et al. 2018

Vet. Med. - Czech

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“…83 Given the formation of well-defined vesicles and biofunctionality of the hydrophilic HYA, they were further able to illustrate the ability for these systems to effectively serve as controlled drug delivery vehicles for the hydrophilic chemotherapeutic, doxorubicin. 83,84 In another report, they illustrated vesicle formation for a similar system, dextran-b-PBLG. 85 More recently, Lecommandoux and coworkers detailed vesicle formation of copolypeptide-based amphiphiles comprised of hydrophobic PBLG and hydrophilic glycosylated propragyl (glycine) (PGC).…”

Section: Assemblies Composed Of Hydrophobic Peptide Coresmentioning

confidence: 97%

“…In general, the helical, rod-like nature of the peptide results in a flatter interface, which favors vesicle of fibril formation. [77][78][79][80][81][82][83][84][85] FIGURE 5 R h vs. pH plots for PB 107 [83][84][85] In their research, they studied assemblies formed from poly(c-benzyl-L-glutamate) 23 -b-hyaluronan 10 (PBLG 23 -b-HYA 10 , polypeptide-bpolysaccharide) polysaccharide. They denote that the PBLG block was chosen to guarantee the formation of vesicles, whereby its rigid, helical nature leads to close packing of helices which serves to favor flatter interface morphologies (Fig.…”

Section: Assemblies Composed Of Hydrophobic Peptide Coresmentioning

confidence: 99%

Self‐assembly and responsiveness of polypeptide‐based block copolymers: How “Smart” behavior and topological complexity yield unique assembly in aqueous media

Ray¹,

Johnson²,

Savin³

2013

J Polym Sci B Polym Phys

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Polypeptide-based amphiphilic block copolymers are an attractive class of materials given their ability to form welldefined aqueous nanoassemblies that respond to external stimulus through secondary structure transitions. This report will highlight recent literature in the area of polypeptide-based block copolymer self-assembly, with the major focus being on how the responsive nature and structural complexity of the polypeptide blocks can be incorporated into systems with complex topologies such as ABA/BAB/ABC triblock copolymers, AB 2 and A 2 B star copolymers, and miktoarm l-ABC star terpolymers. In particular, the role of interfacial curvature changes and how they result in morphology transitions will be discussed. The 'smart' assembly properties of peptides in complex block copolymer topologies can lead to enhanced responsiveness, morphological complexity, and unique morphological transitions with varying solution conditions. V C 2013 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2013, 51, 508-523 KEYWORDS: amphiphiles; biomimetic; block copolymers; selfassembly INTRODUCTION Amphiphilic block copolymers are able to self-assemble into well-defined nanostructures in aqueous solution. [1][2][3][4][5] The equilibrium morphology and aggregation number of diblock copolymer assemblies is primarily determined by the balance of three energetic factors: (1) interfacial tension, (2) corona chain crowding, and (3) core chain stretching. The balance of these three factors dictates an equilibrium curvature for the aggregate. 6-8 Typically, solution morphologies formed by amphiphilic block copolymers follow a trend of increasing interfacial curvature. As the hydrophilic fraction is increased in the copolymer, vesicles, cylindrical micelles, and spherical micelles (in the order of increasing interfacial curvature,) are the most common morphologies (Figure 1). 5,9,10 Physically, this is explained as a balance between entropic freedom of the hydrophilic coronal chains and shielding of the hydrophobic blocks from the aqueous solution; as the hydrophilic fraction increases, the chains are more able to effectively stabilize these assemblies without close-packing, and the free energy of the system is lowered when the coronal chains are provided more entropic freedom/mobility through increased curvature.

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“…Polymersomes that have assembled from amphiphilic block copolymers have proven to be useful tools as drug delivery systems [8,[20][21][22], nanoreactors and sensors [13,17,[23][24][25][26][27][28]. The attachment of targeting ligands or enzymes to the polymersomes, as well as the immobilization of polymer vesicles on surfaces, is of crucial importance in most of the previously mentioned applications.…”

Section: Conjugation To Preformed Polymersomesmentioning

confidence: 99%

“…Mishra and Lecommandoux et al synthesized poly(-benzyl L-glutamate)-block-hyaluronan (PBLG-b-HYA) by -click‖ coupling of PBLG-azide (M n ~ 5 kDa) and HYA-alkyne (Mw ~ 5 kDa), which self-assembled into polymersomes in dilute aqueous solution [22]. The intracellular delivery of doxorubicin loaded polymersomes (PolyDOX) was investigated in high (MCF-7) and low (U87) CD44 expressing cancer cell models.…”

Section: Formation Of Polymersomes From Polymers Comprising Biofunctimentioning

confidence: 99%

Functionalization of Block Copolymer Vesicle Surfaces

et al. 2011

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Abstract:In dilute aqueous solutions certain amphiphilic block copolymers self-assemble into vesicles that enclose a small pool of water with a membrane. Such polymersomes have promising applications ranging from targeted drug-delivery devices, to biosensors, and nanoreactors. Interactions between block copolymer membranes and their surroundings are important factors that determine their potential biomedical applications. Such interactions are influenced predominantly by the membrane surface. We review methods to functionalize block copolymer vesicle surfaces by chemical means with ligands such as antibodies, adhesion moieties, enzymes, carbohydrates and fluorophores. Furthermore, surface-functionalization can be achieved by self-assembly of polymers that carry ligands at their chain ends or in their hydrophilic blocks. While this review focuses on the strategies to functionalize vesicle surfaces, the applications realized by, and envisioned for, such functional polymersomes are also highlighted.

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The intracellular drug delivery and anti tumor activity of doxorubicin loaded poly(γ-benzyl l-glutamate)-b-hyaluronan polymersomes

Cited by 306 publications

References 44 publications

A simplified superovulation protocol using split-single administration of Folltropin®-V in hyaluronan: application to purebred sheep

A simplified superovulation protocol using split-single administration of Folltropin®-V in hyaluronan: application to purebred sheep

Self‐assembly and responsiveness of polypeptide‐based block copolymers: How “Smart” behavior and topological complexity yield unique assembly in aqueous media

Functionalization of Block Copolymer Vesicle Surfaces

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