The intracellular behavior of<i>Torulopsis glabrata</i>

Howard, Dexter H.; Otto, V

doi:10.1080/00362176785190451

Cited by 8 publications

(14 citation statements)

References 11 publications

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“…The mean generation times at these two temperatures were about 2.0 and 0.62 h, respectively. The latter result is somewhat divergent from that of 46 min previously reported by Howard and Otto (22).…”

Section: Resultscontrasting

confidence: 77%

“…2.5 X 3.5 p) which multiplies rapidly. It has a generation time of less than 1 h (22) and frequently develops two or more buds simultaneously. Although budding fungi, notably Saccharomyces cerevisiae, have long been a popular subject for electron microscopy, no work has, to our knowledge, been published on the ultrastructure of T. glabrata.…”

Section: Introductionmentioning

confidence: 99%

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Observations on budding cells of Torulopsis glabrata

Brown¹,

Edwards²

1971

Can. J. Microbiol.

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BROWN, J. P., and M. R. EDWARDS. 1971. Observations on budding cells of Tonrlopsis glabrata. Can. J.Microbiol. 17: 609-616. The diminutive pathogenic yeast Torulopsis glabrata was studied by light and electron microscopy, with special attention to the budding process. Polysaccharides were also extracted to determine glycogen in cytoplasmic particles. Elliptical single cells, with a mean volume of 13 ~m 3 , possess ultrastructural features common in other yeasts, viz. nucleus with double membrane and pores, nucleolus, endoplasmic reticulum, mitochondria, vacuoles, lipid bodies, ribosomes, tripartite plasma membrane, and fibrillar wall with outer electron-dense layer. Numerous pocket-like invaginations of varying size and orientation are formed by the plasma membrane. Buds seem to be preceded by an elaboration of such invaginations and by tubular profiles of the endoplasmic reticulum present in the area of the emerging bud. As the bud protrudes, it appears bounded by a layer of newly synthesized wall material. In general, this process is similar to that in other yeasts (e.g., Saccharornyces) but no vesicles were observed in the peripheral cytoplasm of the mother cell during the initial stages of budding. Various stages in the budding cycle are illustrated.

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Section: Resultscontrasting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Observations on budding cells of Torulopsis glabrata

Brown¹,

Edwards²

1971

Can. J. Microbiol.

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“…Inhibition of T. glabrata by normal serum has been reported previously (Summers and Hasenclever, 1964;Howard and Otto, 1967;Otto and Howard, 1976). We have also observed that T. glabrata was inhibited in media containing 10% human serum (Petrou and Rogers, 1985); and we found that growth was restored in the presence of some antibiotics and subinhibitory concentrations of antifungal drugs.…”

Section: Introductionmentioning

confidence: 84%

The inhibitory effect of serum on the growth of Torulopsis glabrata

Petrou

Rogers²

1988

Journal of Medical Microbiology

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Summary.Normal human plasma and serum were found to inhibit the growth of Torulopsis glabrata and, to a lesser extent, other yeasts. The factor responsible for the inhibition of T. glabrata was not dialysable, was heat stable at 56" C for up to 4 h and could be partly removed by absorption with viable T. glabrata but not Candida albicans. It was fungistatic at low concentrations and fungicidal at high concentrations, stable up to 4 years between -20" C and -70" C, but for only a few weeks at 4" C. Studies with Cohn fractions of serum showed that the inhibitory components were in either the alpha or beta globulin fraction or both. The combined effects of transferrin and IgM accounted for about 70% of the total inhibition observed. We were unable to identify the component responsible for the residual inhibition of growth. The inhibitory effect was totally neutralised by tetracyclines, quinolones, sulphamethoxazole and by very low concentrations of polyenes, imidazoles and 5-fluorocytosine.

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“…Histoplasma capsulatum, Cryptococcus neoformans and Blastomyces dermatitidis were reported to continue their growth within macrophage endocytic vacuoles [7,10,16,20], whereas Torulopsis glabrata could survive but not multiply in these vacuoles [ 11]. The virulence of these micro-organisms is correlated to their ability to survive and multiply intracellularly [22].…”

Section: Discussionmentioning

confidence: 99%