The intestinal response to vitamin D

Bikle, Daniel D.; Morrissey, Robert L.; Zolock, David T.; Rasmussen, Howard

doi:10.1007/bfb0035265

Cited by 44 publications

(18 citation statements)

References 394 publications

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“…7), one of the events associated with the establishment of the osteoclast phenotype. Finally, it should be noted that the action of the steroid on differentiation is apparently not restricted to the mononuclear phagocyte family; alteration in the proliferation and differentiation of gut mucosal cells in response to the vitamin has also been reported (29 …”

Section: Cellsmentioning

confidence: 99%

Induction of monocytic differentiation and bone resorption by 1,25-dihydroxyvitamin D3.

Bar‐Shavit¹,

Teitelbaum²,

Reitsma³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

358

142

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1,25-Dihydroxyvitamin D3 [1,25(0H)2D3] stimulates bone resorption in man and other vertebrates, in part, by increasing the number of osteoclasts, the principal resorbing cells of bone. Because osteoclasts are very likely derived from a member(s) of the mononuclear phagocyte family, we determined if 1,25(OH)2D3 promotes maturation of these cells by studying its effects on the human promyelocytic leukemia cell line HL-60. Of the vitamin D3 metabolites tested, only 1,25(OH)2D3, at 10-10 to 10-7 M, induces the differentiation of HL60 into mono-and multinucleated macrophage-like cells. Phenotypic change is evident within 24 hr and reaches a plateau between 72 and 96 hr of incubation. The changes are metabolite-specific and include (i) adherence to substrate, (ii) acquisition of the morphological features of mature monocytes, (iii) a 4-to 6-fold enhancement in lysozyme synthesis and secretion, (iv) increase in the fraction of a-naphthyl acetate esterase-positive cells from approximately 2% to 100% of the population, and (v) the acquisition of several monocyte-associated cell surface antigens. More importantly, treated HL-60 cells acquire the capacity to bind and degrade bone matrix, two of the essential, functional characteristics of osteoclasts and related boneresorbing cells. These results, considered together with the reported action of 1,25(OH)2D3 on nontransformed mononuclear cells, are consistent with the view that vitamin D3 enhances bone resorption and osteoclastogenesis in vivo by promoting the differentiation of precursor cells.Of the several circulating factors known to affect bone resorption, one of the most potent is 1,25-dihydroxyvitamin D3 [1,25(OH)2D3]. This compound, when administered in picomolar to nanomolar concentrations, markedly stimulates resorptive activity (1) and promotes a readily measurable increase in the number of osteoclasts, the principal resorbing cells of bone (2). It is generally assumed that the appearance of increased numbers of osteoclasts is responsible for enhanced bone resorption. However, the mechanism(s) by which 1,25(OH)2D3 alters the size of the osteoclast population, and therefore resorptive activity, is presently unknown.Osteoclasts originate by fusion of circulating mononuclear precursor cells and almost certainly represent one of the endstage cells of mononuclear phagocyte differentiation (3, 4). Like osteoclasts, other mature, nonproliferative members of this family-e.g., monocytes and macrophages (Mos)-possess the capacity to attach to and degrade bone matrix (5, 6), and therefore they serve as useful models with which to study the mechanisms of bone resorption in tissue culture. In a previous study, we showed that Mos isolated from vitamin D-deficient animals exhibit, in vitro, the same bone resorptive dysfunction characteristically observed in the intact, calciferol-deprived animal (7). This dysfunction is not corrected by the direct addition of 1,25(OH)2D3 to M4 cultures, but administration of the metabolite to vitamin D-depleted animals for several days ...

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Section: Cellsmentioning

confidence: 99%

Induction of monocytic differentiation and bone resorption by 1,25-dihydroxyvitamin D3.

Bar‐Shavit¹,

Teitelbaum²,

Reitsma³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

358

142

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“…Primary cultures of cerebellar granule cells maintained in elevated K+ (.20 mM) and cytosine arabinoside (10 ,uM) contain <10o glial cells. Cultures enriched in glial cells (primarily astrocytes and <5% granule cells) were prepared by reducing the [K+] to 5 mM and omitting cytosine arabinoside (8).…”

Section: Methodsmentioning

confidence: 99%

“…Experiments using isolated kidney tubules or brush-border membranes demonstrate that the physiological regulation of proximal tubule Pi transport is complex, as a variety of hormonal and metabolic factors are known to alter the activity or expression of transporter molecules, or both (1)(2)(3)(4). Although a vitamin D3-regulated Na+-dependent Pi cotransporter has been characterized in the intestine (5), in general, very little is known about Na+-dependent Pi cotransport in other tissues.…”

mentioning

confidence: 99%

Cloning and expression of a cDNA encoding a brain-specific Na(+)-dependent inorganic phosphate cotransporter.

Rosteck

Nadi

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

282

293

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We have isolated a brain-specific cDNA that encodes a Na+-dependent inorganic phosphate (Po) Subtractive Cloning. Subtractive hybridization was used to identify genes differentially expressed after NMDA exposure (12 hr) of cerebellar granule cells. Rat cerebellar granule cells (P8) were cultured and exposed to NMDA as described (6). Poly(A)+ RNA was isolated by using oligo(dT)-cellulose columns (9) from NMDA-treated (12 hr) (tester) and control (untreated) cerebellar granule cells (driver). The tester mRNA (1 I.g) was used to generate[32P]dCTP-labeled first-strand cDNA (6000 Ci/mmol; NEN; 1 Ci = 37 GBq). Driver mRNA (10 pg) was photobiotinylated with a 300 W Sylvania clear bulb and was used to hybridize to tester cDNAs. Subtractive hybridization was done by using subtractor 1, as described by the manufacturer (Invitrogen). After hybridization, common sequences were removed by adding streptavidin and extracting with phenol/chloroform. Resulting subtracted cDNA was used to screen a Zap II cDNA library constructed from NMDA-treated cerebellar granule cells. Positive clones were rescued, replated individually, and rescreened with labeled, single-stranded cDNAs derived from driver and tester mRNAs. Those cDNAs that were detected only by tester probes, and were not detected by driver probes, were isolated for further analysis. Plasmids were excised from A phage, as described by the manufacturer (Stratagene), digested with EcoRI, and electrophoresed in a 1% agarose gel before DNA blotting. cDNA inserts that were specifically detected by tester probes were sequenced.DNA Sequencing and Sequence Analysis. The nucleotide sequence of the BNPI cDNA clone was determined for both strands. Sequence reactions were done by using doublestranded DNA templates, sequence-specific oligonucleotide primers, fluorescently labeled dideoxynucleotide terminators (Applied Biosystems) and Ampli-Taq polymerase in cyclesequencing reactions modified as described (10). Individual sequences were assembled with an Applied Biosystems Abbreviations: NMDA, N-methyl-D-aspartate; BNPI, brain Na+-dependent inorganic phosphate cotransporter I. tPresent address:

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“…Also a rise in cellular cal cium can affect the actin-gelsolin interaction within these macrophages [32]. In the intestinal mucosal cell, 1.25-(OH)2D3 stimulates the entry of calcium across the brush border as well as increases the intracellular cyclic AMP [33]. In dispersed bovine parathyroid cells, addition of l,25-(OH)2D3 results in a rapid rise in cytosol calcium [34], A similar event could take place within the perito neal macrophages.…”

Section: Discussionmentioning

confidence: 99%

1,25-Dihydroxycholecalciferol and Peritoneal Macrophage Chemotaxis in Patients on Continuous Ambulatory Peritoneal Dialysis

Chan

Pun

1991

Nephron

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Single graded doses of 1,25-dihydroxycholecalciferol of 2 and 4 μg were added intraperitoneally into the overnight 1.5% glucose dialysate of 6 patients on continuous ambulatory peritoneal dialysis. The effect on peritoneal macrophage chemotaxis and random migration was studied and compared with the baseline when no 1,25-(OH)₂D₃was added. No consistent effect on peritoneal macrophage chemotaxis was observed. Random migration was significantly depressed at 4 μg when compared with baseline (5.4 ± 1.9 vs. 12.2 ± 3.7 cells/high-power field, p < 0.05). The potential clinical role of 1,25-(OH)₂D₃ as an immune modulator requires further study.

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The intestinal response to vitamin D

Cited by 44 publications

References 394 publications

Induction of monocytic differentiation and bone resorption by 1,25-dihydroxyvitamin D3.

Induction of monocytic differentiation and bone resorption by 1,25-dihydroxyvitamin D3.

Cloning and expression of a cDNA encoding a brain-specific Na(+)-dependent inorganic phosphate cotransporter.

1,25-Dihydroxycholecalciferol and Peritoneal Macrophage Chemotaxis in Patients on Continuous Ambulatory Peritoneal Dialysis

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