The Intestinal Microbiome after Traumatic Injury

Munley, Jennifer A.; Kirkpatrick, Stacey L; Gillies, Gwendolyn S; Bible, Letitia; Efron, Philip A.; Nagpal, Ravinder; Mohr, Alicia M.

doi:10.3390/microorganisms11081990

Cited by 7 publications

(2 citation statements)

References 102 publications

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“…The composition of gut microbiota can undergo shifts after injury due to dysregulation of immune function and inflammation [ 27 ]. Therefore, an initial injury resulting from cannulation surgery may also influence the composition of rumen microbiota in Hanwoo steers.…”

Section: Resultsmentioning

confidence: 99%

Impact of rumen cannulation surgery on rumen microbiota composition in Hanwoo steers

Kim,

Park,

Park

et al. 2024

J Anim Sci Technol

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Rumen cannulation is a surgical technique used to collect rumen contents from ruminants. However, rumen cannulation surgery may potentially impact the composition of the rumen microbiota. This study aimed to examine the longitudinal alterations in the rumen microbiota composition of Hanwoo steers after cannulation surgery. In this study, eight Hanwoo steers were used; four steers underwent rumen cannulation surgery (cannulation group), while the remaining four were left intact (control group). Rumen samples were collected from all eight steers using the stomach tubing method on the day before surgery (day 0) and on postoperative days 1, 4, 7, 10, 14, 17, 21, 24, and 28, resulting in 80 samples (10 timepoints × 8 animals). The microbiota of all 80 samples were analyzed using 16S rRNA gene amplicon sequencing with Quantitative Insights into Microbial Ecology version 2 (QIIME2). There were no significant differences ( p > 0.05) in all major phyla and most major genera representing at least 0.5% of total sequences across all 80 samples between the control and cannulation groups on the preoperative and postoperative days. However, while the alpha diversity indices did not differ ( p > 0.05) between the two groups on the preoperative day, they significantly differed ( p < 0.05) between the two groups on the postoperative days. Further, the overall microbial distribution based on both unweighted and weighted principal coordinate analysis plots significantly differed ( p < 0.05) between the two groups on both the preoperative and postoperative days. Orthogonal polynomial contrasts indicated that major genera and microbial diversity in the cannulation group decreased following surgery but returned to their initial states by postoperative day 28. In conclusion, this study demonstrates that rumen cannulation surgery affects some major taxa and microbial diversity, suggesting that the rumen cannulation method can alter the composition of rumen microbiota in Hanwoo steers.

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Section: Resultsmentioning

confidence: 99%

Impact of rumen cannulation surgery on rumen microbiota composition in Hanwoo steers

Kim,

Park,

Park

et al. 2024

J Anim Sci Technol

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“…4 Trauma and sepsis can induce gut dysbiosis and instigate a pathobiome, which can contribute to subsequent organ dysfunction and delayed recovery. [5][6][7] This dysregulates downstream microbial functions through metabolites in the gut and circulation. 8 Thus, it is critical to assess the persistence or resilience of microbiome-metabolomic dysbiosis after insults, including trauma and sepsis.…”

mentioning

confidence: 99%

Persistence and Sexual Dimorphism of Gut Dysbiosis and Pathobiome after Sepsis and Trauma

Munley,

Park,

Kelly

et al. 2024

Annals of Surgery

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Objective: To evaluate the persistence of intestinal microbiome dysbiosis and gut-plasma metabolomic perturbations following severe trauma or sepsis weeks after admission in patients experiencing chronic critical illness (CCI). Summary: Trauma and sepsis can lead to gut dysbiosis and alterations in the plasma and fecal metabolome. However, the impact of these perturbations and correlations between gut dysbiosis and the plasma metabolome in chronic critical illness have not been studied. Methods: A prospective observational cohort study was performed with healthy subjects, severe trauma patients, patients with sepsis residing in an intensive care unit (ICU) for 2-3 weeks. A high-throughput multi-omics approach was utilized to evaluate the gut microbial and gut-plasma metabolite responses in critically ill trauma and sepsis patients 14-21 days after ICU admission. Results: Patients in the sepsis and trauma cohorts demonstrated strikingly depleted gut microbiome diversity, with significant alterations and specific pathobiome patterns in the microbiota composition compared to healthy subjects. Further subgroup analyses based on sex revealed resistance to changes in microbiome diversity among female trauma patients compared to healthy counterparts. Sex-specific changes in fecal metabolites were also observed after trauma and sepsis, while plasma metabolite changes were similar in both males and females. Conclusions: Dysbiosis induced by trauma and sepsis persists up to 14-21 days after onset and is sex-specific, underscoring the implication of pathobiome and entero-septic microbial-metabolite perturbations in post-sepsis and post-trauma CCI. This indicates resilience to infection or injury in females’ microbiome and should inform and facilitate future precision/personalized medicine strategies in the intensive care unit.

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MKK3 depletion attenuates intestinal injury after traumatic hemorrhagic shock by restoring mitochondrial function

Li,

Zhang,

Kuai

et al. 2024

Mol Biol Rep

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The Intestinal Microbiome after Traumatic Injury

Cited by 7 publications

References 102 publications

Impact of rumen cannulation surgery on rumen microbiota composition in Hanwoo steers

Impact of rumen cannulation surgery on rumen microbiota composition in Hanwoo steers

Persistence and Sexual Dimorphism of Gut Dysbiosis and Pathobiome after Sepsis and Trauma

MKK3 depletion attenuates intestinal injury after traumatic hemorrhagic shock by restoring mitochondrial function

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