The intervention effect of nicotine on cervical fibroblast-myofibroblast differentiation in lipopolysaccharide-induced preterm birth model through activating the TGF-β1/Smad3 pathway

Han, Xinjia; Cai, Chen; Huang, Jiezhen; Li, Qiufen; Huang, Liu; Xuan, Qingshan; Yang, Jinying

doi:10.1016/j.biopha.2020.111135

Cited by 5 publications

(3 citation statements)

References 36 publications

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“…To address the infection related PTL, several mouse models have been developed using bacteria directly ( E. coli ) or LPS, the toxic component on the surface of gram‐negative bacteria. LPS induces PTL and preterm birth (PTB) 41–48 …”

Section: Discussionmentioning

confidence: 99%

“…In particular, M1 macrophages produce proinflammatory cytokines LPS induces PTL and preterm birth (PTB). [41][42][43][44][45][46][47][48] Treatment of mice with LPS is widely used to mimic human PTL. Thus, in this study, we used bacterial LPS to induce PTL in an established model that mimics infection; whether this also replicates other inflammatory events that lead to PTL remains to be investigated.…”

Section: Smc-cm Induce Macrophage Function Through Cxcl12/cxcr4 Signa...mentioning

confidence: 99%

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Myometrial‐derived CXCL12 promotes lipopolysaccharide induced preterm labour by regulating macrophage migration, polarization and function in mice

Zhang

Mamillapalli

Habata

et al. 2022

J Cellular Molecular Medi

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Preterm birth is a major contributor to neonatal mortality and morbidity. Infection results in elevation of inflammation‐related cytokines followed by infiltration of immune cells into gestational tissue. CXCL12 levels are elevated in preterm birth indicating it may have a role in preterm labour (PTL); however, the pathophysiological correlations between CXCL12/CXCR4 signalling and premature labour are poorly understood. In this study, PTL was induced using lipopolysaccharide (LPS) in a murine model. LPS induced CXCL12 RNA and protein levels significantly and specifically in myometrium compared with controls (3‐fold and 3.5‐fold respectively). Highest levels were found just before the start of labour. LPS also enhanced the infiltration of neutrophils, macrophages and T cells, and induced macrophage M1 polarization. In vitro studies showed that condition medium from LPS‐treated primary smooth muscle cells (SMC) induced macrophage migration, M1 polarization and upregulated inflammation‐related cytokines such as interleukin (IL)‐1, IL‐6 and tumor necrosis factor alpha (TNF‐α). AMD3100 treatment in pregnant mice led to a significant decrease in the rate of PTL (70%), prolonged pregnancy duration and suppressed macrophage infiltration into gestation tissue by 2.5‐fold. Further, in‐vitro treatment of SMC by AMD3100 suppressed the macrophage migration, decreased polarization and downregulated IL‐1, IL‐6 and TNF‐α expression. LPS treatment in pregnant mice induced PTL by increasing myometrial CXCL12, which recruits immune cells that in turn produce inflammation‐related cytokines. These effects stimulated by LPS were completely reversed by AMD3100 through blocking of CXCL12/CXCR4 signalling. Thus, the CXCL12/CXCR4 axis presents an excellent target for preventing infection and inflammation‐related PTL.

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Section: Discussionmentioning

confidence: 99%

Section: Smc-cm Induce Macrophage Function Through Cxcl12/cxcr4 Signa...mentioning

confidence: 99%

Myometrial‐derived CXCL12 promotes lipopolysaccharide induced preterm labour by regulating macrophage migration, polarization and function in mice

Zhang

Mamillapalli

Habata

et al. 2022

J Cellular Molecular Medi

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“…Calponin was highly expressed in mature smooth muscle cells and increased with the maturation process (Hossain et al., 2003), but decreased when smooth muscle cells transformed from contractile type to synthetic type (Wang et al., 2020). As a microfilament of the actin family, α‐SMA contained the specificity that can regulate cell morphology and is mainly expressed in differentiated mature vascular smooth muscle cells, of which the expression changes can reflect the smooth muscle cell contractility (Han et al., 2020). The results from this study showed that EAP rats were accompanied by a down‐regulation of the calponin and α‐SMA protein expression in penile tissue, which was in accordance with the concentration changes of PTHS.…”

Section: Discussionmentioning

confidence: 99%

Infiltration of inflammatory factors induced penile damage in chronic prostatitis

Wang

Mao

et al. 2021

Andrologia

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The present study aimed to investigate the mechanism of penile damages in experimental autoimmune prostatitis (EAP) rat models to reveal the potential pathological mechanism of the relationship between CP and penile damages. Sprague–Dawley (SD) rats were administered with different concentrations of prostate tissue homogenate supernatant (PTHS) by multipoint subcutaneous injection to establish EAP models. IHC staining was done to assess the expression of inflammatory cytokines in prostate tissues and the corpus cavernosum of penis. Masson and Tunel staining was conducted to observe the fibrosis and apoptosis in the corpus cavernosum. Finally, the functional changes of corpus cavernosum were assessed by WB and IHC staining. The results revealed that EAP rats with different prostatitis severity were successfully established by PTHS. The expression of IL‐1β, IL‐6 and TNF‐α in prostate tissues increased with the concentration of PTHS. The results of Masson and Tunel staining indicated fibrosis and apoptosis gradually aggravated in corpus cavernosum among different subgroups. The function of cavernosum impaired by prostatitis from WB and IHC results and positively with the severity. In conclusion, there existed the infiltration of inflammatory factors and impaired function in the corpus cavernosum of EAP rats’ penis and positively correlated with the severity of prostatitis.

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Role of Lysocardiolipin Acyltransferase in Cigarette Smoke-Induced Lung Epithelial Cell Mitochondrial ROS, Mitochondrial Dynamics, and Apoptosis

Bandela

Suryadevara

et al. 2021

Cell Biochem Biophys

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The intervention effect of nicotine on cervical fibroblast-myofibroblast differentiation in lipopolysaccharide-induced preterm birth model through activating the TGF-β1/Smad3 pathway

Cited by 5 publications

References 36 publications

Myometrial‐derived CXCL12 promotes lipopolysaccharide induced preterm labour by regulating macrophage migration, polarization and function in mice

Myometrial‐derived CXCL12 promotes lipopolysaccharide induced preterm labour by regulating macrophage migration, polarization and function in mice

Infiltration of inflammatory factors induced penile damage in chronic prostatitis

Role of Lysocardiolipin Acyltransferase in Cigarette Smoke-Induced Lung Epithelial Cell Mitochondrial ROS, Mitochondrial Dynamics, and Apoptosis

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