The interplay of glutathione-related processes in antioxidant defense

Cnubben, N.H.P.; Rietjens, Ivonne M. C. M.; Wortelboer, Heleen M.; Zanden, Jelmer J. van; Bladeren, P.J. van

doi:10.1016/s1382-6689(01)00077-1

Cited by 302 publications

(163 citation statements)

References 84 publications

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“…Under oxidative stress conditions, ROS are reduced by GSH to GSSG catalyzed by GPx. GSH can also consume rapidly and non-enzymatically with hydroxyl radical, NO, N 2 O 3 and peroxynitrite (Cnubben et al, 2001). The HCB-induced GSH depletion was also found by former studies.…”

Section: Gsh and Gsh-related Enzymes In Brainsupporting

confidence: 63%

Effects of hexachlorobenzene on antioxidant status of liver and brain of common carp (Cyprinus carpio)

2006

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Section: Gsh and Gsh-related Enzymes In Brainsupporting

confidence: 63%

Effects of hexachlorobenzene on antioxidant status of liver and brain of common carp (Cyprinus carpio)

2006

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“…The decreased testosterone levels in CR mice may account for their induction, which may further decrease the level of the active steroid. CR induced Gstm2 mRNA, which codes for one of two subunits of an enzyme that detoxifies compounds such as the breakdown products of lipid peroxidation, DNA hydroperoxides, electrophilic xenobiotics, and reactive intermediates formed during their biotransformation (42).…”

Section: Function Of Genes Temporally Associated With Onset Of the Anmentioning

confidence: 99%

Temporal linkage between the phenotypic and genomic responses to caloric restriction

Dhahbi

Kim

Mote

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

228

196

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Caloric restriction (CR), the consumption of fewer calories while avoiding malnutrition, decelerates the rate of aging and the development of age-related diseases. CR has been viewed as less effective in older animals and as acting incrementally to slow or prevent age-related changes in gene expression. Here we demonstrate that CR initiated in 19-month-old mice begins within 2 months to increase the mean time to death by 42% and increase mean and maximum lifespans by 4.7 (P ‫؍‬ 0.000017) and 6.0 months (P ‫؍‬ 0.000056), respectively. The rate of age-associated mortality was decreased 3.1-fold. Between the first and second breakpoints in the CR survival curve (between 21 and 31 months of age), tumors as a cause of death decreased from 80% to 67% (P ‫؍‬ 0.012). Genome-wide microarray analysis of hepatic RNA from old control mice switched to CR for 2, 4, and 8 weeks showed a rapid and progressive shift toward the gene expression profile produced by long-term CR. This shift took place in the time frame required to induce the health and longevity effects of CR. Shifting from long-term CR to a control diet, which returns animals to the control rate of aging, reversed 90% of the gene expression effects of long-term CR within 8 weeks. These results suggest a cause-and-effect relationship between the rate of aging and the CR-associated gene expression biomarkers. Therefore, therapeutics mimicking the gene-expression biomarkers of CR may reproduce its physiological effects.C aloric restriction (CR), the consumption of fewer calories while avoiding malnutrition, is a robust method of decelerating aging and the development of age-related diseases (1). The effects of CR are conserved in nearly every species tested, perhaps including humans (2). CR delays the onset and reduces the incidence and severity of age-related diseases, including cancer (2, 3).Quantitative changes in the activity of genes can control the rate of aging and the development of age-related diseases in invertebrates and mammals (4, 5). Numerous cross-sectional studies of the relationship between CR and gene expression have been published (6). Almost without exception, they have been interpreted as though they were performed longitudinally. This has led to the widespread view that the major effect of CR is to prevent agerelated changes in gene expression. Funding and publication bias has reinforced this notion (6).We previously found that in old mice, a 4-week shift from long-term control (LT-CON) to short-term CR (ST-CR) reproduced 55% of the gene-expression changes induced by long-term CR (LT-CR; ref. 7). ST-CR reversed Ϸ70% of the age-related changes in gene expression seemingly prevented by LT-CR (7). These results suggested for the first time that many of the gene expression changes produced by LT-CR respond rapidly to diet.The studies described above left several important questions unanswered. First, which of the changes in gene expression are related to the health and longevity effects of CR? Further, what are the kinetics of the changes in gene exp...

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“…Therefore, GSTs play central roles in the detoxification of endogenous and xenobiotic compounds, including drugs, herbicides and insecticides (Huang et al 2011). GSTs also participate in the biosynthesis and intracellular transport of hormones and in the protection against oxidative stress (Hayes et al 2005;Cnubben et al 2001). GSTs are ubiquitous in both prokaryotes and eukaryotes.…”

Section: Introductionmentioning

confidence: 99%

A novel Omega-class glutathione S-transferase gene in Apis cerana cerana: molecular characterisation of GSTO2 and its protective effects in oxidative stress

Zhang

Yan

et al. 2013

Cell Stress and Chaperones

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Oxidative stress may be the most significant threat to the survival of living organisms. Glutathione S-transferases (GSTs) serve as the primary defences against xenobiotic and peroxidative-induced oxidative damage. In contrast to other well-defined GST classes, the Omega-class members are poorly understood, particularly in insects. Here, we isolated and characterised the GSTO2 gene from Apis cerana cerana (AccGSTO2). The predicted transcription factor binding sites in the AccGSTO2 promoter suggested possible functions in early development and antioxidant defence. Real-time quantitative PCR (qPCR) and western blot analyses indicated that AccGSTO2 was highly expressed in larvae and was predominantly localised to the brain tissue in adults. Moreover, AccGSTO2 transcription was induced by various abiotic stresses. The purified recombinant AccGSTO2 exhibited glutathione-dependent dehydroascorbate reductase and peroxidase activities. Furthermore, it could prevent DNA damage. In addition, Escherichia coli overexpressing AccGSTO2 displayed resistance to long-term oxidative stress exposure in disc diffusion assays. Taken together, these results suggest that AccGSTO2 plays a protective role in counteracting oxidative stress.

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The interplay of glutathione-related processes in antioxidant defense

Cited by 302 publications

References 84 publications

Effects of hexachlorobenzene on antioxidant status of liver and brain of common carp (Cyprinus carpio)

Effects of hexachlorobenzene on antioxidant status of liver and brain of common carp (Cyprinus carpio)

Temporal linkage between the phenotypic and genomic responses to caloric restriction

A novel Omega-class glutathione S-transferase gene in Apis cerana cerana: molecular characterisation of GSTO2 and its protective effects in oxidative stress

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