The Intermolecular Cross-Links in Uterine Collagens of Guinea Pig, Pig, Cow, and Human Beings

Kao, Kung Ying Tang; Hitt, William E.; Leslie, James G.

doi:10.3181/00379727-151-39217

Cited by 5 publications

(2 citation statements)

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“…This in turn suggests that, although the valvular tissues are of identical animal ages, they are undergoing different rates of collagenous turnover. For instance, tissues shown to have a high rate of collagen turnover, such as the uterus (24), have a larger proportion of immature cross-links (25), whereas a lower rate of collagen turnover results in a larger proportion of mature cross-links (3,52). The synthesis and degradation process effectively "resets" the tissue to a form that is more lightly cross-linked wherein the cross-links are predominantly immature.…”

Section: Table 4 Moles Of Cross-links Per Mole Of Collagen In the 4 mentioning

confidence: 98%

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Differences in collagen cross-linking between the four valves of the bovine heart: a possible role in adaptation to mechanical fatigue

Aldous¹,

Veres²,

A³

et al. 2009

American Journal of Physiology-Heart and Circulatory Physiology

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Hydrothermal isometric tension (HIT) testing and high-performance liquid chromatography were used to assess the molecular stability and cross-link population of collagen in the four valves of the adult bovine heart. Untreated and NaBH(4)-treated tissues under isometric tension were heated in a water bath to a 90 degrees C isotherm that was sustained for 5 h. The denaturation temperature (T(d)), associated with hydrogen bond rupture and molecular stability, and the half-time of load decay (t(1/2)), associated with peptide bond hydrolysis and intermolecular cross-linking, were calculated from acquired load/temperature/time data. An unpaired group of samples of the same population was biochemically assayed for the types and quantities of enzymatic cross-links present. Tissues known to endure higher in vivo transvalvular pressures had lower T(d) values, suggesting that molecular stability is inversely related to in vivo loading. The treated inflow valves (mitral and tricuspid) had significantly lower t(1/2) values than did treated outflow valves (aortic and pulmonary), suggesting lower overall cross-linking in the inflow valves. Inflow valves were also found to fail during HIT testing significantly more often than outflow valves, also suggestive of a decreased cross-link population. Inflow valves may be remodeling at a faster rate and may be at an earlier state of molecular "maturity" than outflow valves. At the molecular level, the thermal stability of collagen is associated with in vivo loading and may be influenced by the mature, aldimine-derived cross-link, histidinohydroxylysinonorleucine. We conclude that the valves of the heart utilize differing, location-specific strategies to resist biomechanical fatigue loading.

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Section: Table 4 Moles Of Cross-links Per Mole Of Collagen In the 4 mentioning

confidence: 98%

“…For instance, a high level of immature crosslinks has been shown in human uterine collagen (25), which is known to have a high rate of collagen turnover (46). Tissues with a higher collagen turnover rate also exhibit a slower net loss of immature cross-links because of continued replacement (9).…”

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confidence: 97%

Differences in collagen cross-linking between the four valves of the bovine heart: a possible role in adaptation to mechanical fatigue

Aldous¹,

Veres²,

A³

et al. 2009

American Journal of Physiology-Heart and Circulatory Physiology

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Analysis of the Crosslinking Components in Collagen and Elastin

Robins¹

1982

Methods of Biochemical Analysis

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Biaxial Creep Resistance and Structural Remodeling of the Aortic and Mitral Valves in Pregnancy

et al. 2015

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Pregnancy produces rapid, dramatic volume-overload changes to the maternal circulation. This paper examines pregnancy-induced structural-mechanical changes in bovine aortic and mitral heart valve leaflets. Valve leaflets were harvested from non-pregnant heifers and pregnant cows. Dimensions, biaxial extensibility and creep resistance were assessed and related to changes in the collagen network: histological leaflet and anatomic layer thicknesses plus collagen crimp, and biochemical collagen content. Collagen stability and crosslinking were assessed thermomechanically. Pregnancy altered both aortic and mitral valve leaflets. Both valves demonstrated biphasic changes in leaflet stretch, decreasing in early pregnancy and recovering by late pregnancy. Creep in leaflets from both valves was minimal and decreased even further with pregnancy in the mitral valve. There were valve-specific changes in preconditioning areal extension with pregnancy: increasing in the aortic valve and decreasing in the mitral valve. Leaflet area increased dramatically (84% aortic, 56% mitral), with thickening mainly in the fibrosa, accompanied by increases in collagen content (8% aortic, 16% mitral): together suggesting synthesis of new collagen. Collagen crimp was almost completely lost in pregnancy, with the denaturation temperature decreased by approximately 2 °C. Mature and total crosslinking increased, curiously without a significant increase in immature crosslinking. Mature aortic and mitral heart valve leaflets in the maternal cardiovascular system remodel substantially and similarly-despite their different embryological origins.

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The Intermolecular Cross-Links in Uterine Collagens of Guinea Pig, Pig, Cow, and Human Beings

Cited by 5 publications

References 0 publications

Differences in collagen cross-linking between the four valves of the bovine heart: a possible role in adaptation to mechanical fatigue

Differences in collagen cross-linking between the four valves of the bovine heart: a possible role in adaptation to mechanical fatigue

Analysis of the Crosslinking Components in Collagen and Elastin

Biaxial Creep Resistance and Structural Remodeling of the Aortic and Mitral Valves in Pregnancy

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